Determination of some β-Blockers by Electrochemical Detection on Polycrstalline Gold Electrode after Solid Phase Extraction (SPE)

2015 ◽  
Vol 28 (5) ◽  
pp. 1060-1067 ◽  
Author(s):  
Innocenzo G. Casella ◽  
Rosa Bonito ◽  
Michela Contursi
Keyword(s):  
Solid Phase Extraction ◽  
Electrochemical Detection ◽  
Gold Electrode ◽  
Solid Phase ◽  
Phase Extraction ◽  
Β Blockers

Determination of 4,4'-methylenebis(2-chloroaniline) in urine by liquid chromatography with ion-paired solid-phase extraction and electrochemical detection.

1988 ◽  
Vol 34 (10) ◽  
pp. 2122-2125 ◽  
Author(s):  
A Okayama ◽  
Y Ichikawa ◽  
M Yoshida ◽  
I Hara ◽  
K Morimoto
Keyword(s):  
Liquid Chromatography ◽  
Solid Phase Extraction ◽  
Electrochemical Detection ◽  
Solid Phase ◽  
Methanol Solution ◽  
Urine Samples ◽  
Phase Extraction ◽  
Routine Measurement ◽  
Sensitive Method

Abstract This highly specific and sensitive method for measuring urinary 4,4'-methylenebis(2-chloroaniline) (MBOCA) involves liquid chromatography with electrochemical detection. Before chromatography, urine samples are prepared by ion-paired solid-phase extraction on a disposable octadecylsilica column with acidic methanol solution containing 1-heptanesulfonic acid. This enhances the specificity of the method. Mean overall recovery ranged from 97.1% to 99.5% at added MBOCA concentrations of 20 and 100 micrograms/L in urine. Sensitivity for urine was 1 microgram/L. The intra-assay CV was 2.2% at a MBOCA concentration of 100 micrograms/L. We believe that this method is acceptable for routine measurement of MBOCA in urine from individuals exposed to this industrial chemical.

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