Direct Electrochemical Sensing of Phenazine-1-carboxylic Acid Secreted byPseudomonas chlororaphissubsp.aureofaciensBCRC 11057TUsing Disposable Screen-printed Carbon Electrode

2015 ◽  
Vol 28 (4) ◽  
pp. 846-853 ◽  
Author(s):  
Asif Hameed ◽  
Hong-Wei Pi ◽  
Shih-Yao Lin ◽  
Wei-An Lai ◽  
Li-Sen Young ◽  
...  
2012 ◽  
Vol 161 (1) ◽  
pp. 549-555 ◽  
Author(s):  
Chanpen Karuwan ◽  
Chakrit Sriprachuabwong ◽  
Anurat Wisitsoraat ◽  
Ditsayut Phokharatkul ◽  
Pornpimol Sritongkham ◽  
...  

2018 ◽  
Vol 4 (4) ◽  

Objectives: In this study, a simple electrochemical nano-genosensor has been developed for the rapid and sensitive detection of methylated SEPT9 DNA as a useful biomarker for early colorectal cancer detection or screening. Methods: The process consists of three main steps: (i) the surface modification of screen-printed carbon electrode (SPCEs) with a poly(l-Arg)/RGO composite film followed by immobilizing anti-5-methylcytosine antibody (ii) preparation of probe-modified Fe3O4/TMC/Au nanocomposites for the hybridization with complementary DNA sequences, (iii) capturing methylated DNA target by antibody-modified SPCEs and subsequent electrochemical detection through redox peak currents of gold nanoparticles which generated a concentration-dependent response. Results: The surface modification of the electrode and hybridization with the methylated target were confirmed by cyclic voltammetry (CV) method and differential pulse voltammetry (DPV) was employ for quantitative evaluation of methylated target DNA. Conclusion: The assay showed a wide linear range from 0.01 pM to 1000 pM with a low detection limit of 0.01pM.


2012 ◽  
Vol 571 ◽  
pp. 56-59
Author(s):  
Yu Fang Sha ◽  
Mei Zhao ◽  
Ming Quan Yang ◽  
Hai Xin Bai ◽  
Man Zhao

Biological multilayer films of redox polymer and horseradish peroxidase (HRP) were successfully assembled on a screen-printed carbon electrode using layer-by-layer (LBL) assembled method based on the electrostatic interaction. The screen-printed carbon electrode surface was modified by the positively charged redox polymer, and the negatively charged HRP by LBL method.


2021 ◽  
pp. 130574
Author(s):  
P.E. Resmi ◽  
Jeethu Raveendran ◽  
P.V. Suneesh ◽  
T. Ramanchandran ◽  
Bipin G Nair ◽  
...  

2014 ◽  
Vol 447 ◽  
pp. 162-168 ◽  
Author(s):  
Nicolaj Cruys-Bagger ◽  
Hirosuke Tatsumi ◽  
Kim Borch ◽  
Peter Westh

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