Impact of Electrochemical Impedance Spectroscopy Experimental Variables on Adsorbed Protein Films, as Illustrated by Bovine Serum Albumin

2015 ◽  
Vol 27 (8) ◽  
pp. 1944-1951 ◽  
Author(s):  
Michelle A. MacDonald ◽  
Heather A. Andreas
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Electrochemical Impedance ◽  
Protein Films ◽  
Adsorbed Protein

Electrochemical impedance spectroscopy reveals a new mechanism based on competitive binding between Tris and protein on a conductive biomimetic polydopamine surface

2018 ◽  
Vol 20 (40) ◽  
pp. 25812-25821 ◽  
Author(s):  
Nimisha Singh ◽  
Jyotsnamayee Nayak ◽  
Khushbu Patel ◽  
Suban K. Sahoo ◽  
Rajender Kumar
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Electrochemical Impedance ◽  
Competitive Binding

A novel mechanism was developed to study the interaction of mussel inspired polydopamine surfaces with bovine serum albumin using electrochemical impedance spectroscopy.

scholarly journals Comparison of Gold Nanoparticles Deposition Methods and Their Influence on Electrochemical and Adsorption Properties of Titanium Dioxide Nanotubes

Materials ◽  
2020 ◽  
Vol 13 (19) ◽  
pp. 4269 ◽  
Author(s):  
Ewa Paradowska ◽  
Katarzyna Arkusz ◽  
Dorota G. Pijanowska
Keyword(s):  
Gold Nanoparticles ◽  
Titanium Dioxide ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Photoelectron Spectroscopy ◽  
Bovine Serum ◽  
Electrochemical Impedance ◽  
Electrochemical Characterizations ◽  
Titanium Dioxide Nanotubes ◽  
X Ray

The increasing interest of attachment of gold nanoparticles (AuNPs) on titanium dioxide nanotubes (TNTs) has been devoted to obtaining tremendous properties suitable for biosensor applications. Achieving precise control of the attachment and shape of AuNPs by methods described in the literature are far from satisfactory. This work shows the comparison of physical adsorption (PA), cyclic voltammetry (CV) and chronoamperometry (CA) methods and the parameters of these methods on TNTs properties. The structural, chemical, phase and electrochemical characterizations of TNTs, Au/TNTs, AuNPs/TNTs are carried out using scanning electron microscopy (SEM), electrochemical impedance spectroscopy, X-ray diffraction, X-ray photoelectron spectroscopy. The use of PA methods does not allow the deposition of AuNPs on TNTs. CV allows easily obtaining spherical nanoparticles, for which the diameter increases from 20.3 ± 2.9 nm to 182.3 ± 51.7 nm as a concentration of tetrachloroauric acid solution increase from 0.1 mM to 10 mM. Increasing the AuNPs deposition time in the CA method increases the amount of gold, but the AuNPs diameter does not change (35.0 ± 5 nm). Importantly, the CA method also causes the dissolution of the nanotubes layer from 1000 ± 10.0 nm to 823 ± 15.3 nm. Modification of titanium dioxide nanotubes with gold nanoparticles improved the electron transfer and increased the corrosion resistance, as well as promoted the protein adsorption. Importantly, after the deposition of bovine serum albumin, an almost 5.5-fold (324%) increase in real impedance, compared to TNTs (59%) was observed. We found that the Au nanoparticles—especially those with smaller diameter—promoted the stability of bovine serum albumin binding to the TNTs platform. It confirms that the modification of TNTs with gold nanoparticles allows the development of the best platform for biosensing applications.

Optical Properties of Thin Layers of Bovine Serum Albumin, γ-Globulin, and Hemoglobin*

1986 ◽  
Vol 40 (3) ◽  
pp. 313-318 ◽  
Author(s):  
H. Arwin
Keyword(s):  
Thin Films ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Dielectric Response ◽  
Thin Layers ◽  
Silicon Substrates ◽  
Protein Films ◽  
Effective Medium Theories ◽  
Monomolecular Films

We have used spectroscopic ellipsometry to determine the dielectric functions of thin films of γ-globulin, bovine serum albumin, and hemoglobin in the visible and near-uv photon energy range. We show that both thickness and dielectric function can be resolved for monomolecular films adsorbed on substrates with relatively low polarizability. The data which we consider to be closest to the intrinsic dielectric response of the protein films were obtained on HgTe and HgCdTe substrates. Less resolution was obtained on silicon substrates. For a density-deficient film, we were able to model the dielectric response with effective medium theories, and the void fraction could be determined.

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