Disposable Electrochemical Magnetoimmunosensor for the Determination of Troponin T Cardiac Marker

2012 ◽  
Vol 25 (1) ◽  
pp. 51-58 ◽  
Author(s):  
Berta Esteban-Fernández de Ávila ◽  
Vanessa Escamilla-Gómez ◽  
Susana Campuzano ◽  
María Pedrero ◽  
José M. Pingarrón
Keyword(s):  
Troponin T ◽  
Cardiac Marker ◽  
Electrochemical Magnetoimmunosensor

Clinical application of rapid quantitative determination of cardiac troponin-T in an emergency department setting

Resuscitation ◽  
2001 ◽  
Vol 49 (3) ◽  
pp. 259-264 ◽  
Author(s):  
Harald Herkner ◽  
Ulla Waldenhofer ◽  
Anton N. Laggner ◽  
Marcus Müllner ◽  
Elisabeth Oschatz ◽  
...  
Keyword(s):  
Emergency Department ◽  
Quantitative Determination ◽  
Clinical Application ◽  
Cardiac Troponin ◽  
Troponin T ◽  
Cardiac Troponin T ◽  
Emergency Department Setting

scholarly journals Proteolytic enzyme and inhibitor levels in reindeer (Rangifer tarandus tarandus L.) vs. bovine longissimus muscle, as they relate to ageing rate and response

Rangifer ◽  
1999 ◽  
Vol 19 (1) ◽  
pp. 13 ◽  
Author(s):  
V. M.H. Barnier ◽  
E. Wiklund ◽  
A. Van Dijk ◽  
F. J.M. Smulders ◽  
G. Malmfors
Keyword(s):  
Proteolytic Enzyme ◽  
Shear Force ◽  
Rangifer Tarandus ◽  
Troponin T ◽  
Myofibrillar Protein ◽  
Collagen Content ◽  
Northern Sweden ◽  
Ph Values ◽  
Ageing Rate

<p>Eight reindeer bulls (age 1.5 years) and six Friesian bulls (age 1.5 years) were included in the study for comparison of tenderness. The reindeer were slaughtered at a commercial reindeer slaughter plant in northern Sweden and the Friesian bulls at a commercial slaughter plant in The Netherlands. Samples for determination of calpain/calpastatin activity were taken from the M. longtssimus (LO) within 1 h post mortem (p.m.), and at various times p.m. pH and temperature were registered in LO; ultimate pH values were measured at 24 h p.m. for beef and at 35 h p.m. for reindeer. At day 1 p.m., samples of LO from both carcass sides were excised, divided in two parts, vacuum packaged and stored at 0-2 &deg;C. One part of each muscle was randomly sampled at 1, 3, 7 and 14 days p.m. for determination of shear force, proteolytic enzyme activity, myofibrillar protein degradation, collagen content and heat solubility. pH and temperature fall was faster in reindeer than in beef. Collagen content in reindeer muscle was found to be low but collagen was 4 times less soluble as compared with beef. Reindeer LO was found to be extremely tender, at 3 days p.m. shear force values were only 2-3 kg/cm2 (8-12 kg/cm2 for beef LO). In reindeer meat, the jJ.-calpain levels dropped to about 55% within 3 days. Troponin T and 30 kDa values were not related to changes in tenderness in reindeer meat. Cathepsin activities in reindeer were up to ten times higher than in beef. As in beef, cathepsin B+L levels in reindeer increased during storage, which is probably associated with a decrease in cystatin-like inhibitor levels.</p>

scholarly journals Az N-terminális pro-B natriureticus peptid mérésének korai ismétlése akut szívelégtelenség miatt hospitalizált betegeken

2018 ◽  
Vol 159 (25) ◽  
pp. 1009-1012
Author(s):  
János Tomcsányi ◽  
Miklós Somlói ◽  
Béla Bózsik ◽  
Tamás Frész ◽  
Erzsébet Nagy
Keyword(s):  
Heart Failure ◽  
Acute Heart Failure ◽  
Natriuretic Peptide ◽  
Troponin T ◽  
Left Ventricular Systolic Dysfunction ◽  
Systolic Dysfunction ◽  
Left Ventricular ◽  
Response To Therapy ◽  
Prospective Data

Abstract: Introduction: The determination of natriuretic peptide levels in patients hospitalized for suspected acute heart failure is important for the confirmation of the diagnosis and for the prognosis. Changes in natriuretic peptide levels in response to therapy have a strong prognostic value. Aim: To decide whether repeated natriuretic peptide measurements for acute heart failure show changes that could influence the diagnosis and/or the prognosis. Method: Prospective data collection was carried out of N-terminal pro-B-type natriuretic peptide (NT-proBNP) levels on admission and within 12 hours in patients hospitalized for acute heart failure. Only the data of those patients were analyzed whose symptoms started within 24 hours prior to admission and were due to acute heart failure. Results: The 23 patients whose data we analyzed had an average age of 77.9 ± 8.3 years. Most of them had left ventricular systolic dysfunction with an average ejection fraction of 34.1 ± 3.9%. The time between the start of symptoms and the first measurement was 6.7 ± 2.2 hours, while the time until the repeated determination was 6.5 ± 2.2 hours after the first measurement. The median value of the NT-proBNP levels in the 6 hours control showed an increase from 5064 pg/mL to 8847 pg/mL (p<0.0005), which amounts to a 75 percent increase – mean hs-troponin T showed an increase from 46 ± 25 ng/L to 78 ± 51 ng/L (p<0.002). Conclusions: A significant increase in NT-proBNP levels is to be expected in early repeated measurement after hospital admission. This fact could have diagnostic and prognostic consequences if validated in a larger patient population. Orv Hetil. 2018; 159(25): 1009–1012.

scholarly journals Lower Cardiac Troponin T and I Results in Heparin-Plasma Than in Serum

2000 ◽  
Vol 46 (9) ◽  
pp. 1338-1344 ◽  
Author(s):  
Hugo Stiegler ◽  
Yuriko Fischer ◽  
Jaime F Vazquez-Jimenez ◽  
Jürgen Graf ◽  
Karsten Filzmaier ◽  
...  
Keyword(s):  
Cardiac Troponin ◽  
Heart Surgery ◽  
Troponin I ◽  
Troponin T ◽  
Open Heart Surgery ◽  
Cardiac Troponin T ◽  
Serum Samples ◽  
Coronary Syndrome ◽  
Heparin Plasma

Abstract Background: The use of plasma rather than serum for determination of cardiac troponins can improve turnaround time and potentially avoid incomplete serum separation that may produce falsely increased results. We investigated the influence of incomplete serum separation and the effect of heparin-plasma on cardiac troponin concentrations. Methods: Serum and heparin-plasma samples were drawn simultaneously from 100 patients (50 patients with acute coronary syndrome and 50 patients after open heart surgery) and measured on three different analytical systems, two for determination of cardiac troponin I (cTnI; Abbott AxSYM and Bayer ACS:Centaur) and one for cardiac troponin T (cTnT; Roche Elecsys cTnT STAT). Serum samples were reanalyzed after a second centrifugation to assess the influence of incomplete serum separation. Results: Mean results (± 95% confidence interval) in heparin-plasma compared with serum were 101% ± 2% (AxSYM cTnI), 94% ± 3% (ACS:Centaur cTnI), and 99% ± 3% (Elecsys cTnT). Differences &gt;20% were seen in 11% of results on the ACS:Centaur, 9% of results on Elecsys cTnT, and 2% of results on the AxSYM. For the Elecsys cTnT assay, the magnitude of the difference between serum and plasma was independent of the absolute concentration and confined to individual samples, and was reversed by treatment with heparinase. A second centrifugation had no effect on serum results by any of the assays. Conclusion: The concentrations of troponins measured in heparin-plasma are markedly lower than in serum in some cases.

The Diagnostic Value of Troponin T Level in the Determination of Cardiac Damage in Perinatal Asphyxia Newborns

2015 ◽  
Vol 35 (1) ◽  
pp. 29-36 ◽  
Author(s):  
Ali Yildirim ◽  
Fatih Ozgen ◽  
Birsen Ucar ◽  
Ozkan Alatas ◽  
Neslihan Tekin ◽  
...  
Keyword(s):  
Troponin T ◽  
Perinatal Asphyxia ◽  
Diagnostic Value ◽  
Cardiac Damage
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