Behavioural, morphological and electrophysiological assessment of the effects of type 2 diabetes mellitus on large and small nerve fibres in Zucker diabetic fatty, Zucker lean and Wistar rats

2018 ◽  
Vol 22 (8) ◽  
pp. 1457-1472 ◽  
Author(s):  
E. Garcia-Perez ◽  
T. Schönberger ◽  
M. Sumalla ◽  
B. Stierstorfer ◽  
R. Solà ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Wistar Rats ◽  
Nerve Fibres ◽  
Small Nerve

scholarly journals Arachidonic Acid Has Anti-Inflammatory and Anti-Diabetic Actions In Vitro and In Vivo

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 747-747
Author(s):  
Undurti Das
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Arachidonic Acid ◽  
Acid Treatment ◽  
Wistar Rats ◽  
Anti Inflammatory

Abstract Objectives To evaluate the effect of arachidonic acid (AA) in the pathobiology of chemical-induced type 1 and type 2 diabetes mellitus in experimental animals. Methods In vitro studies were performed using RIN 5F cells and animal studies in 3–4 week old Wistar rats. Alloxan and streptozotocin (STZ) were used to induce type 1 diabetes and STZ employed to induce type 2 diabetes mellitus. RIN5F cell proliferation was measured using MTT assay. Establishment of alloxan and STZ-induced diabetes in animals was confirmed by measuring plasma glucose levels. Plasma insulin, IL-6, TNF levels were measured by ELISA. Expression of cyclo-oxygenase-2, lipoxiygenase, NF-kB and IkB genes was performed in pancreatic and adipose tissues. Results Alloxan and STZ-induced cytotoxicity to RIN5F cells was inhibited by arachidonic acid that was not blocked by both COX and LOX enzymes. Alloxan and STZ-induced type 1 diabetes mellitus and STZ-induced type 2 diabetes was prevented by arachidonic acid treatment. Plasma levels of glucose, insulin, IL-6 and TNF and expressions of NF-kB, IkB, COX-2, LOX in pancrreatic and adipose tissues and lipocalin-2 in adipose tissue were restored to normal by arachidonic acid treatment. AA treatment enhanced plasma lipoxin A4 (LXA4) levels. LXA4 also prevented both type 1 and type 2 diabetes induction by STZ. Conclusions AA prevented the development of both type 1 and type 2 diabetes mellitus in Wistar rats and protected pancreatic beta cells form the cytotoxicity of alloxan and STZ. AA showed strong anti-inflammatory actions. AA seems to bring about its anti-inflammatory and anti-diabetic actions by enhancing LXA4 formation. Funding Sources None.

Evaluation of diphenhydramine in talc induced type 2 diabetes mellitus in Wistar rats

2018 ◽  
Vol 97 ◽  
pp. 652-655 ◽  
Author(s):  
Muhammad Afzal ◽  
Shakir Saleem ◽  
Nalini Singh ◽  
Imran Kazmi ◽  
Ruqaiyah Khan ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Wistar Rats

scholarly journals Superimposed effect of ovariectomy on type 2 diabetes mellitus in Wistar rats

2018 ◽  
Vol 54 (2) ◽  
pp. 129-137 ◽  
Author(s):  
Minerva K. Fahmy ◽  
Hayam G. Sayyed ◽  
Eman A. Abd Elrahim ◽  
Rana T.A. Farag
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Wistar Rats ◽  
Superimposed Effect

scholarly journals Skin Advanced Glycation End Products among Subjects with Type 2 Diabetes Mellitus with or without Distal Sensorimotor Polyneuropathy

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Stella Papachristou ◽  
Kalliopi Pafili ◽  
Grigorios Trypsianis ◽  
Dimitrios Papazoglou ◽  
Konstantinos Vadikolias ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Disability Score ◽  
Perception Threshold ◽  
Sensorimotor Polyneuropathy ◽  
Function Impairment ◽  
Scientific Laboratory ◽  
Small Nerve

Aim of the Study. To examine the correlation between skin AGEs and parameters of distal sensorimotor polyneuropathy (DSPN) in type 2 diabetes mellitus (T2DM). Materials and Methods. We included 132 subjects (88 men) with a mean age of 64.57 years and median T2DM duration of 14.5 years. Skin AGEs were measured with AGE reader mu connect (Diagnoptics) on the dominant arm. The device enables single and automated triplicate measurements: both of these were performed. DSPN was diagnosed through the neuropathy disability score (NDS). Small nerve fibre function was assessed by temperature and pinprick sensation on the foot. Bilateral measurement of the vibration perception threshold (VPT) on the hallux was carried out by using a neurothesiometer (Horwell Scientific Laboratory Supplies). Results. Single and triplicate AGE measurements were positively correlated with each other (Pearson’s correlation coefficient r = 0.991 , 95 % CI = 0.987 -0.994, p < 0.001 ). AGEs were higher among subjects with vs. those without DSPN ( p < 0.001 ). Furthermore, they were higher among subjects with reduced vs. normal temperature sensation ( p < 0.001 ), among subjects with reduced vs. normal pinprick sensation ( p = 0.002 ), among those with abnormal vs. normal monofilament examination ( p < 0.001 ), and among those with abnormal vs. normal VPT ( p < 0.001 ). AGEs were correlated with NDS, VPT, and monofilament score. Conclusions. In T2DM, skin AGEs are increased in the presence of DSPN. This holds true both for large and for small nerve function impairment. Moreover, AGEs are correlated with DSPN severity.

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