Functionalized Phospholipid Molecular Platform: Use for Production of Cationic Fluorescent Lipids

2013 ◽  
Vol 2014 (5) ◽  
pp. 1076-1083 ◽  
Author(s):  
Mathieu Berchel ◽  
Jean-Pierre Haelters ◽  
Damien Afonso ◽  
Alicia Maroto ◽  
Laure Deschamps ◽  
...  
Keyword(s):  
Fluorescent Lipids

scholarly journals The lateral diffusion of lipid probes in the surface membrane of Schistosoma mansoni.

1986 ◽  
Vol 103 (3) ◽  
pp. 807-818 ◽  
Author(s):  
M Foley ◽  
A N MacGregor ◽  
J R Kusel ◽  
P B Garland ◽  
T Downie ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Fluorescence Recovery After Photobleaching ◽  
Surface Membrane ◽  
Biological Significance ◽  
Lateral Diffusion ◽  
Asymmetric Distribution ◽  
Normal Surface ◽  
Membrane Blebs ◽  
Fluorescent Lipids ◽  
Fluorescent Lipid Analogues

The technique of fluorescence recovery after photobleaching was used to measure the lateral diffusion of fluorescent lipid analogues in the surface membrane of Schistosoma mansoni. Our data reveal that although some lipids could diffuse freely others exhibited restricted lateral diffusion. Quenching of lipid fluorescence by a non-permeant quencher, trypan blue, showed that there was an asymmetric distribution of lipids across the double bilayer of mature parasites. Those lipids that diffused freely were found to reside mainly in the external monolayer of the outer membrane whereas lipids with restricted lateral diffusion were located mainly in one or more of the monolayers beneath the external monolayer. Formation of surface membrane blebs allowed us to measure the lateral diffusion of lipids in the membrane without the influence of underlying cytoskeletal structures. The restricted diffusion found on the normal surface membrane of mature parasites was found to be released in membrane blebs. Quenching of fluorescent lipids on blebs indicated that all probes were present almost entirely in the external monolayer. Juvenile worms exhibited lower lateral diffusion coefficients than mature parasites: in addition, the lipids partitioned into the external monolayer. The results are discussed in terms of membrane organization, cytoskeletal contacts, and biological significance.

scholarly journals Generation of lipid polarity in intestinal epithelial (Caco-2) cells: sphingolipid synthesis in the Golgi complex and sorting before vesicular traffic to the plasma membrane.

1990 ◽  
Vol 111 (3) ◽  
pp. 977-986 ◽  
Author(s):  
W van 't Hof ◽  
G van Meer
Keyword(s):  
Cell Surface ◽  
Selective Extraction ◽  
Intestinal Cells ◽  
Intestinal Epithelial ◽  
Surface Polarity ◽  
Vesicular Traffic ◽  
Back Exchange ◽  
Fluorescent Lipids ◽  
C 50

Generation of intestinal epithelial lipid polarity was studied in Caco-2 cells. Confluent monolayers on filters incorporated the exchangeable lipid N-6-NBD-aminocaproyl-sphingosine (C6-NBD-ceramide) from liposomes. The fluorescent ceramide was converted equally to C6-NBD-glucosylceramide and C6-NBD-sphingomyelin, analogues of lipids enriched on the apical and basolateral surface, respectively, of intestinal cells in vivo. Below 16 degrees C, where vesicular traffic is essentially blocked, each fluorescent product accumulated in the Golgi area. At 37 degrees C, 50% had been transported to the cell surface within 0.5 h, as measured by selective extraction of the fluorescent lipids onto BSA in the medium ("back-exchange") at 10 degrees C. Transport to the two surfaces could be assayed separately, as a diffusion barrier existed for both NBD-lipids and BSA. C6-NBD-glucosylceramide was enriched twofold apically, whereas C6-NBD-sphingomyelin was equally distributed over both domains. Polarities did not decrease when 37 degrees C incubations were carried out in the presence of increasing BSA concentrations to trap the fluorescent lipids immediately after their arrival at the cell surface. Within 10 min from the start of synthesis, both products displayed their typical surface polarity. Lipid transcytosis displayed a half time of hours. In conclusion, newly synthesized sphingolipids in Caco-2 cells are sorted before reaching the cell surface. Transcytosis is not required for generating the in vivo lipid polarity.

Distribution and biophysical properties of fluorescent lipids on the surface of adult Schistosoma mansoni

Parasitology ◽  
1996 ◽  
Vol 113 (2) ◽  
pp. 137-143 ◽  
Author(s):  
C. A. Redman ◽  
J. R. Kusel
Keyword(s):  
Schistosoma Mansoni ◽  
Outer Membrane ◽  
Adult Male ◽  
Surface Membrane ◽  
Fluorescent Microscopy ◽  
Biophysical Properties ◽  
Fluorescent Lipids ◽  
Pass Through ◽  
Lipid Compounds ◽  
Outer Monolayer

SUMMARYThe properties of 4 fluorescent lipid compounds in the surface membrane of adult male Schistosoma mansoni worms were examined by fluorescent microscopy and fluorescent recovery after photobleaching (FRAP). The data suggest that the probes N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl) sphingosine (BODIPY FL ceramide) and PKH2 pass through the outer membrane and enter structures in or below the membrane. In contrast 5-(N-octadecanoyl)aminofluorescein (AF18) and N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl) sphingosylphosphocholine (BODIPY FL sphingomyelin) insert into the outer monolayer. The DL values of these latter 2 compounds, 8·83 ± 2·35 × 10−9 cm2 s−1 and 2·76 ± 0·53 × 10−9cm2 s−1, respectively, suggest that they enter different domains. Furthermore, it was observed that both BODIPY FL ceramide and BODIPY FL sphingomyelin entered particular structures in or under the surface membrane. The possible nature of these particles is discussed.

scholarly journals Fluorescent Lipids: Functional Parts of Fusogenic Liposomes and Tools for Cell Membrane Labeling and Visualization

Molecules ◽  
2012 ◽  
Vol 17 (1) ◽  
pp. 1055-1073 ◽  
Author(s):  
Christian Kleusch ◽  
Nils Hersch ◽  
Bernd Hoffmann ◽  
Rudolf Merkel ◽  
Agnes Csiszár
Keyword(s):  
Cell Membrane ◽  
Fluorescent Lipids ◽  
Membrane Labeling

Vital Staining of Cells with Fluorescent Lipids

Cell Biology ◽  
2006 ◽  
pp. 139-145 ◽  
Author(s):  
T KOBAYASHI ◽  
A MAKINO ◽  
K ISHII
Keyword(s):  
Vital Staining ◽  
Fluorescent Lipids

scholarly journals Using fluorescent lipids contributes to the active learning of principles underlying lipid signaling

2018 ◽  
Vol 47 (1) ◽  
pp. 100-105 ◽  
Author(s):  
Micaela Peppino Margutti ◽  
Matias Reyna ◽  
Ana L. Villasuso
Keyword(s):  
Active Learning ◽  
Lipid Signaling ◽  
Fluorescent Lipids
Sign in / Sign up

Export Citation Format

Share Document