CAL-B-Catalyzed Acylation of Nucleosides and Role of the Sugar Conformation: An Improved Understanding of the Enzyme-Substrate Recognition

2012 ◽  
Vol 2012 (28) ◽  
pp. 5483-5490 ◽  
Author(s):  
Saúl Martínez-Montero ◽  
Susana Fernández ◽  
Yogesh S. Sanghvi ◽  
Vicente Gotor ◽  
Miguel Ferrero
Keyword(s):  
Substrate Recognition ◽  
Enzyme Substrate ◽  
Enzyme Substrate Recognition ◽  
Sugar Conformation

scholarly journals Dolichol is not a necessary moiety for lipid-linked oligosaccharide substrates of the mannosyltransferases involved in in vitro N-linked-oligosaccharide assembly

1995 ◽  
Vol 310 (3) ◽  
pp. 909-916 ◽  
Author(s):  
I B H Wilson ◽  
M C Webberley ◽  
L Revers ◽  
S L Flitsch
Keyword(s):  
Biosynthetic Pathway ◽  
Substrate Recognition ◽  
Liver Microsomes ◽  
Rat Liver Microsomes ◽  
Enzyme Substrate ◽  
Low Concentrations ◽  
Enzyme Substrate Recognition

Dolichol is utilized in vivo as an unusually large anchor on which the precursor for N-linked oligosaccharides is assembled by a series of glycosyltransferases. The role of dolichol in enzyme substrate recognition is investigated. Thus the biosynthetic intermediate NN′-diacetylchitobiose was chemically linked to either dolichol or the much shorter fully saturated tetraisoprenoid phytanol. Both lipids were used as substrates by a recombinant, soluble beta-1,4-mannosyltransferase. beta-[3H]Mannosylated lipids from this reaction were then used as substrates for the subsequent mannosyltransferases from yeast or rat liver microsomes. It was found that both the dolichyl- and phytanyl-linked substrates were easily mannosylated to form Man5GlcNAc2, with some further mannosylation to Man7GlcNAc2 and Man9GlcNAc2 at low concentrations of lipid-linked substrate. It is concluded that dolichol is not necessary in vitro as part of the substrate for the mannosyltransferases in the biosynthetic pathway for N-glycosylation.

Transient Enzyme–Substrate Recognition Monitored by Real-Time NMR

2011 ◽  
Vol 133 (29) ◽  
pp. 11154-11162 ◽  
Author(s):  
Caroline Haupt ◽  
Rica Patzschke ◽  
Ulrich Weininger ◽  
Stefan Gröger ◽  
Michael Kovermann ◽  
...  
Keyword(s):  
Real Time ◽  
Substrate Recognition ◽  
Enzyme Substrate ◽  
Enzyme Substrate Recognition

Enzyme-substrate recognition

1978 ◽  
Vol 73 (1) ◽  
pp. 181-204 ◽  
Author(s):  
L. Edelstein ◽  
R. Rosen
Keyword(s):  
Substrate Recognition ◽  
Enzyme Substrate ◽  
Enzyme Substrate Recognition

Probing the Structural Basis for Enzyme-Substrate Recognition In Cu, Zn Superoxide Dismutase

1991 ◽  
Vol 12 (1) ◽  
pp. 287-296 ◽  
Author(s):  
Cindy L. Fisher ◽  
Robert A. Hallewell ◽  
Victoria A. Roberts ◽  
John A. Tainer ◽  
Elizabeth D. Getzoff
Keyword(s):  
Superoxide Dismutase ◽  
Substrate Recognition ◽  
Structural Basis ◽  
Enzyme Substrate ◽  
Enzyme Substrate Recognition
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