A directed mutational approach demonstrates that a putative linoleate isomerase fromLactobacillus acidophilusdoes not hydrate or isomerize linoleic acid

2015 ◽  
Vol 118 (6) ◽  
pp. 841-848 ◽  
Author(s):  
Michael P. C. Fibinger ◽  
G. Johannes Freiherr von Saß ◽  
Cornelia Herrfurth ◽  
Ivo Feussner ◽  
Uwe T. Bornscheuer
Keyword(s):  
Linoleic Acid ◽  
Linoleate Isomerase

scholarly journals Conjugated linoleic acid producing potential of lactobacilli isolated from goat (AXB) rumen fluid samples

2020 ◽  
Vol 33 (8) ◽  
pp. 1233-1241
Author(s):  
Amrish Kumar Tyagi ◽  
Sachin Kumar ◽  
Prasanta Kumar Choudhury ◽  
Bhawna Tyagi ◽  
Nitin Tyagi
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Rumen Fluid ◽  
Spectrophotometric Assay ◽  
Lactobacillus Species ◽  
Rrna Gene ◽  
Chain Reaction ◽  
Specific Polymerase Chain Reaction ◽  
Polymerase Chain ◽  
Linoleate Isomerase

Objective: The present investigation was aimed to explore the potential of lactobacilli for conjugated linoleic acid (CLA) production, isolated from rumen fluid samples of lactating goats.Methods: A total of 64 isolates of lactobacilli were obtained using deMan-Rogosa-Sharpe (MRS) agar from rumen fluid of goats and further subjected to morphological and biochemical characterizations. Isolates found as gram-positive, catalase negative rods were presumptively identified as Lactobacillus species and further confirmed by genus specific polymerase chain reaction (PCR). The phylogenetic tree was constructed from the nucleotide sequences using MEGA6.Results: Out of the 64 isolates, 23 isolates were observed positive for CLA production by linoleate isomerase gene-based amplification and quantitatively by UV-spectrophotometric assay for the conversion of linoleic acid to CLA as well as gas chromatography-based assay. In all Lactobacillus species cis9, trans11 isomer was observed as the most predominant CLA isomer. These positive isolates were identified by 16S rRNA gene-based PCR sequencing and identified to be different species of <i>L. ingluviei</i> (2), <i>L.salivarius</i> (2), <i>L. curvatus</i> (15), and <i>L. sakei</i> (4).Conclusion: The findings of the present study concluded that lactic acid bacteria isolated from ruminal fluid samples of goat have the potential to produce bioactive CLA and may be applied as a direct fed microbial to enhance the nutraceutical value of animal food products.

scholarly journals Synthesis of conjugated linoleic acid by the linoleate isomerase complex in food-derived lactobacilli

2014 ◽  
Vol 117 (2) ◽  
pp. 430-439 ◽  
Author(s):  
B. Yang ◽  
H. Chen ◽  
Z. Gu ◽  
F. Tian ◽  
R. P. Ross ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Linoleate Isomerase

scholarly journals Linoleic acid isomerization ability of Lactobacillus spp. isolated from Vietnamese human intestinal origins

2020 ◽  
Vol 18 (3) ◽  
pp. 445-453
Author(s):  
Tran Xuan Thach ◽  
Ha Thi Thu ◽  
Vu Thi Hien ◽  
Hoang The Hung ◽  
Nguyen Thi Hoa ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Double Bonds ◽  
Liquid Form ◽  
Isomerase Activity ◽  
Acid Isomerization ◽  
Hydroxy Groups ◽  
Substrate Concentrations ◽  
Lactobacillus Spp ◽  
Mrs Medium ◽  
Linoleate Isomerase

Conjugated linoleic acid (CLA) have been shown to exert numerous health benefits, including anti-carcinogenic, anti-atherogenic, anti-diabetic, antiobesity, cholesterol reducing, antioxidant, anti-microbial, immune system modulator and growth-stimulating properties. In human, CLA is produced from Linoleic acid (LA) by gut bacteria. In this study, nineteen Lactobacillus (Lac.) strains isolated from human feces were studied to determine their ability to metabolize LA. The bacteria were grown in the liquid form of anaerobic MRS medium supplemented with 0.5 mg/mL LA. The linoleate isomerase activity in bacteria grown on MRS medium was determined by Gas chromatograpy. The results indicated that 4 out of 19 strains, including strains Lac.02, Lac.05, Lac.14 and Lac.16 are capable of producing about 40-50 μg/mL CLA from LA. Among them, the highest ability to produce CLA from LA is Lac.02 strain. In the production of CLA from LA, enzymes involved in this metabolism in Lactobacillus act as catalysts of hydration/dehydration (CLA-HY), oxidation of hydroxy groups/reduction of oxo groups (CLA-DH), migration of carbon-carbon double bonds (CLA-DC), and saturation of carbon-carbon double bonds (CLA-ER). The cla-dh, cla-dc, cla-hy and cla-er genes that encode enzymes CLA-DH, CLA-DC, and CLA-ER had been found in all Lac.02, Lac.05, Lac.14 and Lac.16 strains. Gas chromatography traces indicated that these strains produced the same compounds, which was subsequently identified as cis-9, trans-11, and trans-10, cis-12 CLA. In the next study, we will optimize the conditions such as substrate concentrations, pH values, temperature and culture time of each strain to obtain the best rerults.

Conjugated Linoleic Acid Causes a Marked Increase in Liver α-Tocopherol and Liver α-Tocopherol Transfer Protein in C57BL/6 J Mice

2010 ◽  
Vol 80 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Pei-Min Chao ◽  
Wan-Hsuan Chen ◽  
Chun-Huei Liao ◽  
Huey-Mei Shaw
Keyword(s):  
Antioxidant Enzymes ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substances ◽  
Control Groups ◽  
Protein Levels ◽  
Transfer Protein ◽  
And Control

Conjugated linoleic acid (CLA) is a collective term for the positional and geometric isomers of a conjugated diene of linoleic acid (C18:2, n-6). The aims of the present study were to evaluate whether levels of hepatic α-tocopherol, α-tocopherol transfer protein (α-TTP), and antioxidant enzymes in mice were affected by a CLA-supplemented diet. C57BL/6 J mice were divided into the CLA and control groups, which were fed, respectively, a 5 % fat diet with or without 1 g/100 g of CLA (1:1 mixture of cis-9, trans-11 and trans-10, cis-12) for four weeks. α-Tocopherol levels in plasma and liver were significantly higher in the CLA group than in the control group. Liver α-TTP levels were also significantly increased in the CLA group, the α-TTP/β-actin ratio being 2.5-fold higher than that in control mice (p<0.01). Thiobarbituric acid-reactive substances were significantly decreased in the CLA group (p<0.01). There were no significant differences between the two groups in levels of three antioxidant enzymes (superoxide dismutase, glutathione peroxidase, and catalase). The accumulation of liver α-tocopherol seen with the CLA diet can be attributed to the antioxidant potential of CLA and the ability of α-TTP induction. The lack of changes in antioxidant enzyme protein levels and the reduced lipid peroxidation in the liver of CLA mice are due to α-tocopherol accumulation.

α-Linoleic acid enhances the capacity of α1-antitrypsin to inhibit lipopolysaccharide-induced IL-1β in human blood neutrophils

Pneumologie ◽  
2016 ◽  
Vol 70 (07) ◽  
Author(s):  
N Aggarwal ◽  
S Wrenger ◽  
V Grau ◽  
T Welte ◽  
S Janciauskiene
Keyword(s):  
Linoleic Acid ◽  
Human Blood ◽  
Blood Neutrophils

36-LB: Linoleic Acid Derived Oxylipins, Memory, and Microvascular Retinal Complications in Patients with Type 2 Diabetes Mellitus

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 36-LB
Author(s):  
SOPHIE K. WONG ◽  
MICHELLE M. NGUYEN ◽  
CHELSI MAJOR-ORFAO ◽  
KRISTA L. LANCTÔT ◽  
NATHAN HERRMANN ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Linoleic Acid ◽  
Retinal Complications
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