Eicosapentaenoic acid (EPA) production by an oleaginous fungusMortierella alpinaexpressing heterologous the Δ17-desaturase gene under ordinary temperature

2015 ◽  
Vol 117 (12) ◽  
pp. 1919-1927 ◽  
Author(s):  
Tomoyo Okuda ◽  
Akinori Ando ◽  
Hiroaki Negoro ◽  
Tatsuya Muratsubaki ◽  
Hiroshi Kikukawa ◽  
...  
Keyword(s):  
Eicosapentaenoic Acid ◽  
Desaturase Gene ◽  
Ordinary Temperature

Identification of a fatty acid ∆6-desaturase gene from the eicosapentaenoic acid-producing fungus Pythium splendens RBB-5

2012 ◽  
Vol 35 (3) ◽  
pp. 431-438 ◽  
Author(s):  
Ruijiao Zhang ◽  
Yuanmin Zhu ◽  
Liang Ren ◽  
Pengpeng Zhou ◽  
Jingrong Hu ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Eicosapentaenoic Acid ◽  
Desaturase Gene ◽  
Pythium Splendens

scholarly journals Increase of Eicosapentaenoic Acid in Thraustochytrids through Thraustochytrid Ubiquitin Promoter-Driven Expression of a Fatty Acid Δ5 Desaturase Gene

2011 ◽  
Vol 77 (11) ◽  
pp. 3870-3876 ◽  
Author(s):  
Takumi Kobayashi ◽  
Keishi Sakaguchi ◽  
Takanori Matsuda ◽  
Eriko Abe ◽  
Yoichiro Hama ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Eicosapentaenoic Acid ◽  
Docosapentaenoic Acid ◽  
Eicosatetraenoic Acid ◽  
Ubiquitin Promoter ◽  
Desaturase Gene ◽  
Content Type ◽  
Δ5 Desaturase ◽  
Marine Protists

ABSTRACTThraustochytrids, marine protists known to accumulate polyunsaturated fatty acids (PUFAs) in lipid droplets, are considered an alternative to fish oils as a source of PUFAs. The major fatty acids produced in thraustochytrids are palmitic acid (C16:0),n− 6 docosapentaenoic acid (DPA) (C22:5n− 6), and docosahexaenoic acid (DHA) (C22:6n− 3), with eicosapentaenoic acid (EPA) (C20:5n− 3) and arachidonic acid (AA) (C20:4n− 6) as minor constituents. We attempted here to alter the fatty acid composition of thraustochytrids through the expression of a fatty acid Δ5 desaturase gene driven by the thraustochytrid ubiquitin promoter. The gene was functionally expressed inAurantiochytrium limacinummh0186, increasing the amount of EPA converted from eicosatetraenoic acid (ETA) (C20:4n− 3) by the Δ5 desaturase. The levels of EPA and AA were also increased by 4.6- and 13.2-fold in the transgenic thraustochytrids compared to levels in the mock transfectants when ETA and dihomo-γ-linolenic acid (DGLA) (C20:3n− 6) were added to the culture at 0.1 mM. Interestingly, the amount of EPA in the transgenic thraustochytrids increased in proportion to the amount of ETA added to the culture up to 0.4 mM. The rates of conversion and accumulation of EPA were much higher in the thraustochytrids than in baker's yeasts when the desaturase gene was expressed with the respective promoters. This report describes for the first time the finding that an increase of EPA could be accomplished by introducing the Δ5 desaturase gene into thraustochytrids and indicates that molecular breeding of thraustochytrids is a promising strategy for generating beneficial PUFAs.

scholarly journals Identification and Functional Characterization of the MossPhyscomitrella patensΔ5-Desaturase Gene Involved in Arachidonic and Eicosapentaenoic Acid Biosynthesis

2006 ◽  
Vol 281 (31) ◽  
pp. 21988-21997 ◽  
Author(s):  
Songsri Kaewsuwan ◽  
Edgar B. Cahoon ◽  
Pierre-François Perroud ◽  
Chanpen Wiwat ◽  
Nathinee Panvisavas ◽  
...  
Keyword(s):  
Eicosapentaenoic Acid ◽  
Functional Characterization ◽  
Acid Biosynthesis ◽  
Desaturase Gene

Eicosapentaenoic Acid Interferes with U46619-Stimulated Formation of Inositol Phosphates in Washed Rabbit Platelets

1989 ◽  
Vol 62 (04) ◽  
pp. 1116-1120 ◽  
Author(s):  
N Chetty ◽  
J D Vickers ◽  
R L Kinlough-Rathbone ◽  
M A Packham ◽  
J F Mustard
Keyword(s):  
Signal Transduction ◽  
Fatty Acid Composition ◽  
Eicosapentaenoic Acid ◽  
Inositol Phosphates ◽  
Inositol Phosphate ◽  
Dense Granule ◽  
Detectable Change ◽  
Membrane Phospholipids ◽  
Rabbit Platelets ◽  
Stimulation Of

SummaryEicosapentaenoic acid (EPA) inhibits platelet responsiveness to aggregating agents. To investigate the reactions that are affected by EPA, we examined the effect of preincubating aspirintreated rabbit platelets with EPA on stimulation of inositol phosphate formation in response to the TXA2 analogue U46619. Stimulation of platelets with U46619 (0.5 μM) caused aggregation and slight release of dense granule contents; aggregation and release were inhibited by preincubation of the platelets with EPA (50 μM) for 1 h followed by washing to remove unincorporated EPA. Incubation with EPA (50 μM) for 1 h did not cause a detectable increase in the amount of EPA in the platelet phospholipids. When platelets were prelabelled with [3H]inositol stimulation with U46619 of control platelets that had not been incubated with EPA significantly increased the labelling of mos1tol phosphates. The increases in inositol phosphate labelling due to U46619 at 10 and 60 s were partially inhibited by premcubat10n of the platelets with 50 μM EPA. Since the activity of cyclo-oxygenase was blocked with aspirin, inhibition of inositol phosphate labelling in response to U46619 indicates either that there may be inhibition of signal transduction without a detectable change in the amount of EPA in platelet phospholipids, that changes in signal transduction require only minute changes in the fatty acid composition of membrane phospholipids, or that after a 1 h incubation with EPA, activation of phospholipase C is affected by a mechanism that is not directly related to incorporation of EPA.

Decrease of Platelet Activity After Intake of Small Amounts of Eicosapentaenoic Acid in Diabetics

1982 ◽  
Vol 48 (03) ◽  
pp. 344-344 ◽  
Author(s):  
B Velardo ◽  
M Lagarde ◽  
M Guichardant ◽  
M Dechavanne ◽  
M Beylot ◽  
...  
Keyword(s):  
Eicosapentaenoic Acid ◽  
Platelet Activity

Dunaliella salina Teod. as a Prominent Source of Eicosapentaenoic Acid

2010 ◽  
Vol 12 (2) ◽  
pp. 185-189 ◽  
Author(s):  
Rahul A. Bhosale ◽  
M. P. Rajabhoj ◽  
B. B. Chaugule
Keyword(s):  
Eicosapentaenoic Acid ◽  
Dunaliella Salina
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