The Electrically Wired Molybdenum Domain of Human Sulfite Oxidase is Bioelectrocatalytically Active

2015 ◽  
Vol 2015 (21) ◽  
pp. 3526-3531 ◽  
Author(s):  
Roberto Spricigo ◽  
Silke Leimkühler ◽  
Lo Gorton ◽  
Frieder W. Scheller ◽  
Ulla Wollenberger
Keyword(s):  
Sulfite Oxidase ◽  
Human Sulfite Oxidase

scholarly journals Low Potential Catalytic Voltammetry of Human Sulfite Oxidase

2016 ◽  
Vol 199 ◽  
pp. 280-289 ◽  
Author(s):  
Palraj Kalimuthu ◽  
Abdel A. Belaidi ◽  
Guenter Schwarz ◽  
Paul V. Bernhardt
Keyword(s):  
Sulfite Oxidase ◽  
Human Sulfite Oxidase

ChemInform Abstract: Kinetic and Thermodynamic Effects of Mutations of Human Sulfite Oxidase

ChemInform ◽  
2012 ◽  
Vol 43 (45) ◽  
pp. no-no
Author(s):  
Asha Rajapakshe ◽  
Gordon Tollin ◽  
John H. Enemark
Keyword(s):  
Sulfite Oxidase ◽  
Thermodynamic Effects ◽  
Human Sulfite Oxidase

scholarly journals Structural Studies of the Molybdenum Center of the Pathogenic R160Q Mutant of Human Sulfite Oxidase by Pulsed EPR Spectroscopy and17O and33S Labeling

2008 ◽  
Vol 130 (26) ◽  
pp. 8471-8480 ◽  
Author(s):  
Andrei V. Astashkin ◽  
Kayunta Johnson-Winters ◽  
Eric L. Klein ◽  
Changjian Feng ◽  
Heather L. Wilson ◽  
...  
Keyword(s):  
Epr Spectroscopy ◽  
Sulfite Oxidase ◽  
Structural Studies ◽  
Pulsed Epr ◽  
Human Sulfite Oxidase

scholarly journals Kinetic results for mutations of conserved residues H304 and R309 of human sulfite oxidase point to mechanistic complexities

Metallomics ◽  
2014 ◽  
Vol 6 (9) ◽  
pp. 1664-1670 ◽  
Author(s):  
Amanda C. Davis ◽  
Kayunta Johnson-Winters ◽  
Anna R. Arnold ◽  
Gordon Tollin ◽  
John H. Enemark
Keyword(s):  
Metabolic Disorder ◽  
Point Mutations ◽  
Sulfite Oxidase ◽  
Oxidase Deficiency ◽  
Conserved Residues ◽  
Inherited Metabolic Disorder ◽  
Sulfite Oxidase Deficiency ◽  
Human Sulfite Oxidase

Several point mutations in the gene of human sulfite oxidase (hSO) result in isolated sulfite oxidase deficiency, an inherited metabolic disorder.

Mechanism of nitrite-dependent NO synthesis by human sulfite oxidase

2019 ◽  
Vol 476 (12) ◽  
pp. 1805-1815 ◽  
Author(s):  
Daniel Bender ◽  
Alexander Tobias Kaczmarek ◽  
Dimitri Niks ◽  
Russ Hille ◽  
Guenter Schwarz
Keyword(s):  
Electron Transfer ◽  
Spectroscopic Studies ◽  
Sulfite Oxidase ◽  
Nitrite Reduction ◽  
Intramolecular Electron Transfer ◽  
Intermembrane Space ◽  
Electron Transfer Mechanism ◽  
Physiological Relevance ◽  
Order Of Magnitude ◽  
Human Sulfite Oxidase

AbstractIn addition to nitric oxide (NO) synthases, molybdenum-dependent enzymes have been reported to reduce nitrite to produce NO. Here, we report the stoichiometric reduction in nitrite to NO by human sulfite oxidase (SO), a mitochondrial intermembrane space enzyme primarily involved in cysteine catabolism. Kinetic and spectroscopic studies provide evidence for direct nitrite coordination at the molybdenum center followed by an inner shell electron transfer mechanism. In the presence of the physiological electron acceptor cytochrome c, we were able to close the catalytic cycle of sulfite-dependent nitrite reduction thus leading to steady-state NO synthesis, a finding that strongly supports a physiological relevance of SO-dependent NO formation. By engineering SO variants with reduced intramolecular electron transfer rate, we were able to increase NO generation efficacy by one order of magnitude, providing a mechanistic tool to tune NO synthesis by SO.

Effects of large-scale amino acid substitution in the polypeptide tether connecting the heme and molybdenum domains on catalysis in human sulfite oxidase

Metallomics ◽  
2010 ◽  
Vol 2 (11) ◽  
pp. 766 ◽  
Author(s):  
Kayunta Johnson-Winters ◽  
Anna R. Nordstrom ◽  
Amanda C. Davis ◽  
Gordon Tollin ◽  
John H. Enemark
Keyword(s):  
Amino Acid ◽  
Amino Acid Substitution ◽  
Large Scale ◽  
Sulfite Oxidase ◽  
Human Sulfite Oxidase

The Pathogenic Human Sulfite Oxidase Mutants G473D and A208D Are Defective in Intramolecular Electron Transfer†

Biochemistry ◽  
2005 ◽  
Vol 44 (42) ◽  
pp. 13734-13743 ◽  
Author(s):  
Changjian Feng ◽  
Heather L. Wilson ◽  
Gordon Tollin ◽  
Andrei V. Astashkin ◽  
James T. Hazzard ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Sulfite Oxidase ◽  
Intramolecular Electron Transfer ◽  
Human Sulfite Oxidase

Optimization of Expression of Human Sulfite Oxidase and Its Molybdenum Domain

2000 ◽  
Vol 383 (2) ◽  
pp. 281-287 ◽  
Author(s):  
Carrie A Temple ◽  
Tyler N Graf ◽  
K.V Rajagopalan
Keyword(s):  
Sulfite Oxidase ◽  
Human Sulfite Oxidase

scholarly journals Effects of Interdomain Tether Length and Flexibility on the Kinetics of Intramolecular Electron Transfer in Human Sulfite Oxidase

Biochemistry ◽  
2010 ◽  
Vol 49 (6) ◽  
pp. 1290-1296 ◽  
Author(s):  
Kayunta Johnson-Winters ◽  
Anna R. Nordstrom ◽  
Safia Emesh ◽  
Andrei V. Astashkin ◽  
Asha Rajapakshe ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Sulfite Oxidase ◽  
Intramolecular Electron Transfer ◽  
Human Sulfite Oxidase ◽  
Kinetics Of
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