Zn2+-Responsive Bimodal Magnetic Resonance Imaging and Fluorescence Imaging Agents and Their Interaction with Human Serum Albumin

2014 ◽  
Vol 2014 (20) ◽  
pp. 3208-3215 ◽  
Author(s):  
Ying-Qi Xu ◽  
Jian Luo ◽  
Zhong-Ning Chen
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Fluorescence Imaging ◽  
Imaging Agents ◽  
Resonance Imaging

scholarly journals Contrast Enhancement of the Brain by Folate-Conjugated Gadolinium–Diethylenetriaminepentaacetic Acid–Human Serum Albumin Nanoparticles by Magnetic Resonance Imaging

2012 ◽  
Vol 11 (4) ◽  
pp. 7290.2011.00047 ◽  
Author(s):  
Huedayi Korkusuz ◽  
Karsten Ulbrich ◽  
Verena Bihrer ◽  
Katerina Welzel ◽  
Valery Chernikov ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Contrast Agents ◽  
Diethylenetriaminepentaacetic Acid ◽  
Resonance Imaging ◽  
Mri Contrast ◽  
The Brain ◽  
Signal Intensities

Different from regular small molecule contrast agents, nanoparticle-based contrast agents have a longer circulation time and can be modified with ligands to confer tissue-specific contrasting properties. We evaluated the tissue distribution of polymeric nanoparticles (NPs) prepared from human serum albumin (HSA), loaded with gadolinium–diethylenetriaminepentaacetic acid (Gd-DTPA) (Gd-HSA-NP), and coated with folic acid (FA) (Gd-HSA-NP-FA) in mice by magnetic resonance imaging (MRI). FA increases the affinity of the Gd-HSA-NP to FA receptor–expressing cells. Clinical 3 T MRI was used to evaluate the signal intensities in the different organs of mice injected with Gd-DTPA, Gd-HSA-NP, or Gd-HSA-NP-FA. Signal intensities were measured and standardized by calculating the signal to noise ratios. In general, the NP-based contrast agents provided stronger contrasting than Gd-DTPA. Gd-HSA-NP-FA provided a significant contrast enhancement (CE) in the brain ( p = .0032), whereas Gd-DTPA or Gd-HSA-NP did not. All studied MRI contrast agents showed significant CE in the blood, kidney, and liver ( p < .05). Gd-HSA-NP-FA elicited significantly higher CE in the blood than Gd-HSA-NP ( p = .0069); Gd-HSA-NP and Gd-HSA-NP-FA did not show CE in skeletal muscle and gallbladder; Gd-HSA-NP, but not Gd-HSA-NP-FA, showed CE in the cardiac muscle. Gd-HSA-NP-FA has potential as an MRI contrast agent in the brain.

Intraoperative Fluorescence Staining of Malignant Brain Tumors Using 5-Aminofluorescein-Labeled Albumin

2009 ◽  
Vol 64 (suppl_1) ◽  
pp. ONS53-ONS61 ◽  
Author(s):  
Paul Kremer ◽  
Mahmoudreza Fardanesh ◽  
Reinhard Ding ◽  
Maria Pritsch ◽  
Saida Zoubaa ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Human Serum Albumin ◽  
Brain Tumors ◽  
Serum Albumin ◽  
Tumor Tissue ◽  
Malignant Gliomas ◽  
Tumor Resection ◽  
Resonance Imaging ◽  
Fluorescence Staining

Abstract Objective: The newly developed conjugate 5-aminofluorescein (AFL)-human serum albumin (HSA) was investigated in a clinical trial for fluorescence-guided surgery of malignant brain tumors to assess its efficacy and tolerability. Methods: AFL, covalently linked to human serum albumin at a molar ratio of 1:1, was administered intravenously 0.5 to 4 days before surgery at 0.5 or 1.0 mg/kg of body weight to 13 patients aged 38 to 71 years who were suspected of having malignant gliomas. Fluorescence guidance using a 488-nm argon laser was performed during surgery at will. The extent of tumor resection was verified by early postoperative magnetic resonance imaging. Fluorescent and nonfluorescent samples were collected for neuropathology. Blood samples for laboratory and pharmacokinetic analyses were taken over the course of 4 weeks. Results: Fluorescence staining of tumor tissue was bright in 11 patients (84%), resulting in complete resection of fluorescent tumor tissue in 9 patients (69%). In 2 patients, residual fluorescent tumor tissue was also confirmed by magnetic resonance imaging. Neither bleaching nor penetration of AFL-HSA into the surrounding brain edema or into necrotic tissue was seen. The agreement between fluorescence and histopathology in tumor samples and samples of the tumor border was 83.3%. There were no toxic side effects. The quality of fluorescence was independent of the dose administered. The optimal time for surgery is between 1 and 4 days after AFL-HSA administration. Conclusion: Tumor fluorescence using AFL-HSA made fluorescence-guided brain tumor resection possible, demonstrating that albumin is a suitable carrier system for selective targeting of aminofluorescein into malignant gliomas.

Toxicity of Magnetic Resonance Imaging Agents: Small Molecule and Nanoparticle

2013 ◽  
Vol 13 (4) ◽  
pp. 434-445 ◽  
Author(s):  
Yongmin Chang ◽  
Gang Ho Lee ◽  
Tae-Jeong Kim ◽  
Kwon-Seok Chae
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Small Molecule ◽  
Imaging Agents ◽  
Resonance Imaging
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