scholarly journals Postarrest stalling rather than crawling favors CD8 + over CD4 + T‐cell migration across the blood–brain barrier under flow in vitro

2016 ◽  
Vol 46 (9) ◽  
pp. 2187-2203 ◽  
Author(s):  
Henriette Rudolph ◽  
Armelle Klopstein ◽  
Isabelle Gruber ◽  
Claudia Blatti ◽  
Ruth Lyck ◽  
...  
Keyword(s):  
Cell Migration ◽  
T Cell ◽  
Blood Brain Barrier ◽  
Cd4 T Cell ◽  
Brain Barrier ◽  
T Cell Migration ◽  
Blood Brain

scholarly journals Brain endothelial tricellular junctions as novel sites for T cell diapedesis across the blood–brain barrier

2021 ◽  
Vol 134 (8) ◽  
Author(s):  
Mariana Castro Dias ◽  
Adolfo Odriozola Quesada ◽  
Sasha Soldati ◽  
Fabio Bösch ◽  
Isabelle Gruber ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Blood Brain Barrier ◽  
Immune Surveillance ◽  
Brain Barrier ◽  
Activated T Cells ◽  
Blood Brain ◽  
Primary Mouse ◽  
Underlying Mechanisms

ABSTRACT The migration of activated T cells across the blood–brain barrier (BBB) is a critical step in central nervous system (CNS) immune surveillance and inflammation. Whereas T cell diapedesis across the intact BBB seems to occur preferentially through the BBB cellular junctions, impaired BBB integrity during neuroinflammation is accompanied by increased transcellular T cell diapedesis. The underlying mechanisms directing T cells to paracellular versus transcellular sites of diapedesis across the BBB remain to be explored. By combining in vitro live-cell imaging of T cell migration across primary mouse brain microvascular endothelial cells (pMBMECs) under physiological flow with serial block-face scanning electron microscopy (SBF-SEM), we have identified BBB tricellular junctions as novel sites for T cell diapedesis across the BBB. Downregulated expression of tricellular junctional proteins or protein-based targeting of their interactions in pMBMEC monolayers correlated with enhanced transcellular T cell diapedesis, and abluminal presence of chemokines increased T cell diapedesis through tricellular junctions. Our observations assign an entirely novel role to BBB tricellular junctions in regulating T cell entry into the CNS. This article has an associated First Person interview with the first author of the paper.

scholarly journals CD152 (CTLA-4) Determines CD4 T Cell Migration In Vitro and In Vivo

PLoS ONE ◽  
2009 ◽  
Vol 4 (5) ◽  
pp. e5702 ◽  
Author(s):  
Karin Knieke ◽  
Holger Hoff ◽  
Frank Maszyna ◽  
Paula Kolar ◽  
Arnhild Schrage ◽  
...  
Keyword(s):  
Cell Migration ◽  
T Cell ◽  
Cd4 T Cell ◽  
T Cell Migration

scholarly journals ACKR1 favors transcellular over paracellular T‐cell diapedesis across the blood‐brain barrier in neuroinflammation in vitro

2021 ◽  
Author(s):  
Luca Marchetti ◽  
David Francisco ◽  
Sasha Soldati ◽  
Neda Haghayegh Jahromi ◽  
Sara Barcos ◽  
...  
Keyword(s):  
T Cell ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Blood Brain
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