scholarly journals A novel Cre recombinase reporter mouse strain facilitates selective and efficient infection of primary immune cells with adenoviral vectors

2015 ◽  
Vol 45 (6) ◽  
pp. 1614-1620 ◽  
Author(s):  
Klaus Heger ◽  
Maike Kober ◽  
David Rieß ◽  
Christoph Drees ◽  
Ingrid de Vries ◽  
...  
Keyword(s):  
Immune Cells ◽  
Mouse Strain ◽  
Cre Recombinase ◽  
Adenoviral Vectors ◽  
Reporter Mouse

scholarly journals Importance of Adult Dmbx1 in Long-Lasting Orexigenic Effect of Agouti-Related Peptide

Endocrinology ◽  
2016 ◽  
Vol 157 (1) ◽  
pp. 245-257 ◽  
Author(s):  
Seiichiro Hirono ◽  
Eun Young Lee ◽  
Shunsuke Kuribayashi ◽  
Takahiro Fukuda ◽  
Naokatsu Saeki ◽  
...  
Keyword(s):  
Mouse Strain ◽  
Energy Homeostasis ◽  
Expression Patterns ◽  
Critical Role ◽  
Fetal Brain ◽  
Cre Recombinase ◽  
Calcitonin Gene Related Peptide ◽  
Adult Brain ◽  
Related Peptide ◽  
Calcitonin Gene

Abstract Dmbx1 is a brain-specific homeodomain transcription factor expressed primarily during embryogenesis, and its systemic disruption (Dmbx1−/−) in the ICR mouse strain resulted in leanness associated with impaired long-lasting orexigenic effect of agouti-related peptide (AgRP). Because spatial and temporal expression patterns of Dmbx1 change dramatically during embryogenesis, it remains unknown when and where Dmbx1 plays a critical role in energy homeostasis. In the present study, the physiological roles of Dmbx1 were examined by its conditional disruption (Dmbx1loxP/loxP) in the C57BL/6 mouse strain. Although Dmbx1 disruption in fetal brain resulted in neonatal lethality, its disruption by synapsin promoter-driven Cre recombinase, which eliminated Dmbx1 expression postnatally, exempted the mice (Syn-Cre;Dmbx1loxP/loxP mice) from lethality. Syn-Cre;Dmbx1loxP/loxP mice show mild leanness and impaired long-lasting orexigenic action of AgRP, demonstrating the physiological relevance of Dmbx1 in the adult. Visualization of Dmbx1-expressing neurons in adult brain using the mice harboring tamoxifen-inducible Cre recombinase in the Dmbx1 locus (Dmbx1CreERT2/+ mice) revealed Dmbx1 expression in small numbers of neurons in restricted regions, including the lateral parabrachial nucleus (LPB). Notably, c-Fos expression in LPB was increased at 48 hours after AgRP administration in Dmbx1loxP/loxP mice but not in Syn-Cre;Dmbx1loxP/loxP mice. These c-Fos-positive neurons in LPB did not coincide with neurons expressing Dmbx1 or melanocortin 4 receptor but did coincide with those expressing calcitonin gene-related peptide. Accordingly, Dmbx1 in the adult LPB is required for the long-lasting orexigenic effect of AgRP via the neural circuitry involving calcitonin gene-related peptide neurons.

scholarly journals INTEGRINS MEDIATE PLACENTAL EXTRACELLULAR VESICLE TRAFFICKING TO LUNG AND LIVER IN VIVO

2020 ◽  
Author(s):  
Sean L. Nguyen ◽  
Soo Hyun Ahn ◽  
Jacob W. Greenberg ◽  
Benjamin W. Collaer ◽  
Dalen W. Agnew ◽  
...  
Keyword(s):  
Immune Cells ◽  
Extracellular Vesicle ◽  
Dependent Manner ◽  
Cellular Phenotype ◽  
Reporter Mouse ◽  
Membrane Bound ◽  
First Time

ABSTRACTMembrane-bound extracellular vesicles (EVs) mediate intercellular communication in all organisms, and those produced by placental mammals have become increasingly recognized as significant mediators of fetal-maternal communication. Here, we aimed to identify maternal cells targeted by placental EVs and elucidate the mechanisms by which they traffic to these cells. Exogenously administered pregnancy-associated EVs traffic specifically to the lung; further, placental EVs associate with lung interstitial macrophages and liver Kupffer cells in an integrin-dependent manner. Localization of EV to maternal lungs was confirmed in unmanipulated pregnancy using a transgenic reporter mouse model, which also provided in situ and in vitro evidence that fetally-derived EVs, rarely, may cause genetic alteration of maternal cells. These results provide for the first time direct in vivo evidence for targeting of placental EVs to maternal immune cells, and further, evidence that EVs can alter cellular phenotype.

scholarly journals Immune cell-specific Cre reporter mouse labels a subset of CNS neurons

2019 ◽  
Author(s):  
Aurélie Bouteau ◽  
Botond Z. Igyártó
Keyword(s):  
Immune Cells ◽  
Langerhans Cells ◽  
Immune Cell ◽  
Neuronal Marker ◽  
Reporter Mouse ◽  
Cns Neurons ◽  
The Central Nervous System ◽  
Antigen Presenting

AbstractHuLangerin-Cre-YFPf/f mice were generated to specifically mark a subset of antigen presenting immune cells, called Langerhans cells (LCs). During histological characterization of these mice, we found that, in addition to LCs an uncharacterized cell population in the central nervous system (CNS) also expressed YFP. In this study, we found that the CNS YFP+ cells were negative for microglia and astrocyte markers, but they expressed mature neuronal marker NeuN and showed neuronal localization/morphology. Thus, these mice might be used to study the ontogeny, migration and the role of a subset of CNS neurons.

Construction of an sIgE:FLAG-mIgE:GFP Reporter Mouse Strain

2003 ◽  
Vol 130 (4) ◽  
pp. 280-287 ◽  
Author(s):  
Gertrude Achatz-Straussberger ◽  
Roland Geisberger ◽  
Iris Oberndorfer ◽  
Daniela Inführ ◽  
Elke Luger ◽  
...  
Keyword(s):  
Mouse Strain ◽  
Reporter Mouse

IL-12 p40-Expressing Immune Cells Revealed by Cytokine Reporter Mouse System

2009 ◽  
Vol 22 (1) ◽  
pp. 95
Author(s):  
Mia Gi ◽  
Jungeun Kim ◽  
Wooseok Im ◽  
Jeehee Youn ◽  
Seokmann Hong
Keyword(s):  
Immune Cells ◽  
Reporter Mouse ◽  
Mouse System

scholarly journals A unique mouse strain expressing Cre recombinase for tissue-specific analysis of gene function in palate and kidney development

genesis ◽  
2007 ◽  
Vol 45 (10) ◽  
pp. 618-624 ◽  
Author(s):  
Yu Lan ◽  
Qingru Wang ◽  
Catherine E. Ovitt ◽  
Rulang Jiang
Keyword(s):  
Gene Function ◽  
Mouse Strain ◽  
Kidney Development ◽  
Cre Recombinase ◽  
Tissue Specific ◽  
Specific Analysis

scholarly journals Variation in the immune response to adenoviral vectors in the brain: influence of mouse strain, environmental conditions and priming

Gene Therapy ◽  
1999 ◽  
Vol 6 (4) ◽  
pp. 471-481 ◽  
Author(s):  
Y Ohmoto ◽  
M J A Wood ◽  
H M Charlton ◽  
K Kajiwara ◽  
V H Perry ◽  
...  
Keyword(s):  
Immune Response ◽  
Mouse Strain ◽  
Environmental Conditions ◽  
Adenoviral Vectors ◽  
The Brain

scholarly journals A novel reporter mouse strain that expresses enhanced green fluorescent protein upon Cre-mediated recombination

FEBS Letters ◽  
2000 ◽  
Vol 470 (3) ◽  
pp. 263-268 ◽  
Author(s):  
Shunsuke Kawamoto ◽  
Hitoshi Niwa ◽  
Fumi Tashiro ◽  
Shigetoshi Sano ◽  
Gen Kondoh ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Mouse Strain ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Reporter Mouse ◽  
Green Fluorescent
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