scholarly journals Intraendosomal flow cytometry: A novel approach to analyze the protein composition of antigen-loaded endosomes

2012 ◽  
Vol 42 (8) ◽  
pp. 2187-2190 ◽  
Author(s):  
Matthias Zehner ◽  
Judith Rauen ◽  
Achmet I. Chasan ◽  
Maria Embgenbroich ◽  
Marcel G. Camps ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Protein Composition ◽  
Novel Approach

A novel approach for the selection of human sperm using annexin V-binding and flow cytometry

2009 ◽  
Vol 91 (4) ◽  
pp. 1285-1292 ◽  
Author(s):  
Christiaan F. Hoogendijk ◽  
Theunis F. Kruger ◽  
Patric J.D. Bouic ◽  
Ralf R. Henkel
Keyword(s):  
Flow Cytometry ◽  
Annexin V ◽  
Human Sperm ◽  
Novel Approach ◽  
Selection Of

In Situ Imaging of Cancer Cell via Cancer Aptamer and its Fluorescent Clusters

2014 ◽  
Vol 998-999 ◽  
pp. 256-259
Author(s):  
Lu Ga ◽  
Jun Ai
Keyword(s):  
Flow Cytometry ◽  
Cancer Cell ◽  
Mtt Assay ◽  
Cellular Protein ◽  
Silver Nanoclusters ◽  
Cell Toxicity ◽  
Novel Approach ◽  
Diphenyltetrazolium Bromide ◽  
Ag Nanoclusters

Silver nanoclusters synthesized using aptamer as matrices attracted more attention in the fields of medicine, pharmacology and biology. In this paper, we report a novel approach to sgc-8-C8-templated formation of fluorescent Ag nanoclusters and its application to bioimaging. This was further confirmed by flow cytometry. The cell toxicity (3-[4, 5-dimethylthiazolyl-2]-2, 5 -diphenyltetrazolium bromide, MTT) assay demonstrated that the silver nanoclusters has only little affect on the cytotoxicity to the cells. These results demonstrated that this aptamer-based method for labeling and imaging cellular protein is facile, effective.

scholarly journals Flow cytometry for near-patient testing in premature neonates reveals variation in platelet function: a novel approach to guide platelet transfusion

2019 ◽  
Vol 85 (6) ◽  
pp. 874-884 ◽  
Author(s):  
Amie K. Waller ◽  
Lajos Lantos ◽  
Audrienne Sammut ◽  
Burak Salgin ◽  
Harriet McKinney ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Platelet Function ◽  
Platelet Transfusion ◽  
Premature Neonates ◽  
Novel Approach ◽  
Near Patient Testing

scholarly journals Characterization of an Acellular Scaffold for a Tissue Engineering Approach to the Nipple-Areolar Complex Reconstruction

2017 ◽  
Vol 203 (3) ◽  
pp. 183-193 ◽  
Author(s):  
Nicholas C. Pashos ◽  
Michelle E. Scarritt ◽  
Zachary R. Eagle ◽  
Jeffrey M. Gimble ◽  
Abigail E. Chaffin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Protein Composition ◽  
Structural Protein ◽  
Nipple Areolar Complex ◽  
Engineering Approach ◽  
Areolar Complex ◽  
Novel Approach ◽  
Number Of Patients ◽  
Tissue Engineering Approach ◽  
Quantitative Analyses

A significant number of patients undergo mastectomies and breast reconstructions every year using many surgical-based techniques to reconstruct the nipple-areolar complex (NAC). Described herein is a tissue engineering approach that may permit a human NAC onlay graft during breast reconstruction procedures. By applying decellularization, which is the removal of cellular components from tissue, to an intact whole donor NAC, the extracellular matrix (ECM) structure of the NAC is preserved. This creates a biologically derived scaffold for cells to repopulate and regenerate the NAC. A detergent-based decellularization method was used to derive whole NAC scaffolds from nonhuman primate rhesus macaque NAC tissue. Using both histological and quantitative analyses for the native and decellularized tissues, the derived ECM graft was assessed. The bioactivity of the scaffold was evaluated following cell culture with bone marrow-derived mesenchymal stem cells (BMSCs). The data presented here demonstrate that scaffolds are devoid of cells and retain ECM integrity and a high degree of bioactivity. The content of collagen and glycosaminoglycans were not significantly altered by the decellularization process, whereas the elastin content was significantly decreased. The proliferation and apoptosis of seeded BMSCs were found to be approximately 65 and <1.5%, respectively. This study characterizes the successful decellularization of NAC tissue as compared to native NACs based on structural protein composition, lubricating protein retention, the maintenance of adhesion molecules, and bioactivity when reseeded with cells. These histological and quantitative analyses provide the foundation for a novel approach to NAC reconstruction.

scholarly journals Glycosome heterogeneity in kinetoplastids

2021 ◽  
Author(s):  
Logan P. Crowe ◽  
Meredith T. Morris
Keyword(s):  
Flow Cytometry ◽  
Protein Import ◽  
Therapeutic Targets ◽  
Protein Composition ◽  
Recent Advances ◽  
Cellular Machinery ◽  
And Function ◽  
Organelle Sorting

Kinetoplastid parasites have essential organelles called glycosomes that are analogous to peroxisomes present in other eukaryotes. While many of the processes that regulate glycosomes are conserved, there are several unique aspects of their biology that are divergent from other systems and may be leveraged as therapeutic targets for the treatment of kinetoplastid diseases. Glycosomes are heterogeneous organelles that likely exist as sub-populations with different protein composition and function in a given cell, between individual cells, and between species. However, the limitations posed by the small size of these organelles makes the study of this heterogeneity difficult. Recent advances in the analysis of small vesicles by flow-cytometry provide an opportunity to overcome these limitations. In this review, we describe studies that document the diverse nature of glycosomes and propose an approach to using flow cytometry and organelle sorting to study the diverse composition and function of these organelles. Because the cellular machinery that regulates glycosome protein import and biogenesis is likely to contribute, at least in part, to glycosome heterogeneity we highlight some ways in which the glycosome protein import machinery differs from that of peroxisomes in other eukaryotes.

scholarly journals Separation of myeloid and erythroid progenitors based on expression of CD34 and c-kit

Blood ◽  
1995 ◽  
Vol 86 (11) ◽  
pp. 4076-4085 ◽  
Author(s):  
MO De Jong ◽  
G Wagemaker ◽  
AW Wognum
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
Bone Marrow Cells ◽  
Low Frequency ◽  
Erythroid Progenitors ◽  
Color Flow ◽  
Novel Approach ◽  
Colony Forming Units ◽  
The Difference

In this report, a novel approach is described to physically separate erythroid progenitors from monocyte and granulocyte progenitors, based on the expression of CD34 and Kit. Using biotin-labeled human Kit ligand (KL) and flow cytometry, Kit was detectable on 2% to 3% of the nucleated cells in rhesus monkey bone marrow. Combination of biotin-KL with CD34 monoclonal antibodies (MoAb) showed that Kit was expressed on subsets of CD34low and CD34pos cells. Our data clearly demonstrate that CD34pos cells are more heterogeneous with respect to Kit expression than observed in studies using Kit MoAb. A small cluster, approximately 7% of the CD34pos cells, expressed CD34 at submaximal levels and stained brightly with biotinylated KL. This CD34pos/kithi fraction contained predominantly erythroid progenitors (burst-forming units- erythroid; BFU-E). The majority of the granulocytic and monocytic progenitors (colony-forming units-granulocyte/macrophage; CFU-GM) were CD34pos/kitmed. Some BFU-E were also detected in the CD34pos/kitmed and CD34low/kitpos fractions at low frequency. In the latter subset, most erythroid colony-forming units (CFU-E) were recovered. Using three- color flow cytometry, we analyzed expression of Kit in relation to that of CD34 and the class II major histocompatibility antigen, RhLA-DR. The most immature bone marrow cells that can be identified in vitro, ie, CD34pos/RhLA-DRlow cells, were kitmed. The CD34pos/kithi and CD34pos/kitneg subsets predominantly contained the more mature RhLA- DRbright cells. Our results demonstrate that erythroid precursors express c-kit at much higher levels than monomyeloid precursors and pluripotent progenitors. The difference in expression levels of CD34 and c-kit can be exploited to isolate BFU-E populations that are virtually devoid of nonerythroid cells.

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