scholarly journals The thiol redox state of lymphoid organs is modified by immunization: Role of different immune cell populations

2008 ◽  
Vol 38 (9) ◽  
pp. 2419-2425 ◽  
Author(s):  
Patrizia Castellani ◽  
Giovanna Angelini ◽  
Laura Delfino ◽  
Andrea Matucci ◽  
Anna Rubartelli
Keyword(s):  
Redox State ◽  
Immune Cell ◽  
Lymphoid Organs ◽  
Cell Populations ◽  
Thiol Redox State ◽  
Thiol Redox

scholarly journals Detecting intracellular thiol redox state in leukaemia and heterogeneous immune cell populations: An optimised protocol for digital flow cytometers

MethodsX ◽  
2018 ◽  
Vol 5 ◽  
pp. 1473-1483 ◽  
Author(s):  
Alex J. Wadley ◽  
Rhys G. Morgan ◽  
Kate J. Heesom ◽  
Paul S. Hole ◽  
Steven J. Coles
Keyword(s):  
Redox State ◽  
Immune Cell ◽  
Cell Populations ◽  
Thiol Redox State ◽  
Thiol Redox

scholarly journals The Effectiveness of Glutathione Redox Status as a Possible Tumor Marker in Colorectal Cancer

2021 ◽  
Vol 22 (12) ◽  
pp. 6183
Author(s):  
Delia Acevedo-León ◽  
Lidia Monzó-Beltrán ◽  
Segundo Ángel Gómez-Abril ◽  
Nuria Estañ-Capell ◽  
Natalia Camarasa-Lillo ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Redox State ◽  
Reduced Glutathione ◽  
Redox Status ◽  
Oxidation Products ◽  
Interventional Study ◽  
Thiol Redox State ◽  
Thiol Redox ◽  
Glutathione Redox

The role of oxidative stress (OS) in cancer is a matter of great interest due to the implication of reactive oxygen species (ROS) and their oxidation products in the initiation of tumorigenesis, its progression, and metastatic dissemination. Great efforts have been made to identify the mechanisms of ROS-induced carcinogenesis; however, the validation of OS byproducts as potential tumor markers (TMs) remains to be established. This interventional study included a total of 80 colorectal cancer (CRC) patients and 60 controls. By measuring reduced glutathione (GSH), its oxidized form (GSSG), and the glutathione redox state in terms of the GSSG/GSH ratio in the serum of CRC patients, we identified significant changes as compared to healthy subjects. These findings are compatible with the effectiveness of glutathione as a TM. The thiol redox state showed a significant increase towards oxidation in the CRC group and correlated significantly with both the tumor state and the clinical evolution. The sensitivity and specificity of serum glutathione levels are far above those of the classical TMs CEA and CA19.9. We conclude that the GSSG/GSH ratio is a simple assay which could be validated as a novel clinical TM for the diagnosis and monitoring of CRC.

scholarly journals Dnmt3b regulates DUX4 expression in a tissue-dependent manner in transgenic D4Z4 mice

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Linde F. Bouwman ◽  
Bianca den Hamer ◽  
Elwin P. Verveer ◽  
Lente J. S. Lerink ◽  
Yvonne D. Krom ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Immune Cell ◽  
Lymphoid Organs ◽  
Cell Populations ◽  
Lymphoid Tissues ◽  
Dependent Manner ◽  
Transcript Levels ◽  
Repeat Array ◽  
D4z4 Repeat

Abstract Background Facioscapulohumeral muscular dystrophy (FSHD) is a skeletal muscle disorder that is caused by derepression of the transcription factor DUX4 in skeletal muscle cells. Apart from SMCHD1, DNMT3B was recently identified as a disease gene and disease modifier in FSHD. However, the exact role of DNMT3B at the D4Z4 repeat array remains unknown. Methods To determine the role of Dnmt3b on DUX4 repression, hemizygous mice with a FSHD-sized D4Z4 repeat array (D4Z4-2.5 mice) were cross-bred with mice carrying an in-frame exon skipping mutation in Dnmt3b (Dnmt3bMommeD14 mice). Additionally, siRNA knockdowns of Dnmt3b were performed in mouse embryonic stem cells (mESCs) derived from the D4Z4-2.5 mouse model. Results In mESCs derived from D4Z4-2.5 mice, Dnmt3b was enriched at the D4Z4 repeat array and DUX4 transcript levels were upregulated after a knockdown of Dnmt3b. In D4Z4-2.5/Dnmt3bMommeD14 mice, Dnmt3b protein levels were reduced; however, DUX4 RNA levels in skeletal muscles were not enhanced and no pathology was observed. Interestingly, D4Z4-2.5/Dnmt3bMommeD14 mice showed a loss of DNA methylation at the D4Z4 repeat array and significantly higher DUX4 transcript levels in secondary lymphoid organs. As these lymphoid organs seem to be more sensitive to epigenetic modifiers of the D4Z4 repeat array, different immune cell populations were quantified in the spleen and inguinal lymph nodes of D4Z4-2.5 mice crossed with Dnmt3bMommeD14 mice or Smchd1MommeD1 mice. Only in D4Z4-2.5/Smchd1MommeD1 mice the immune cell populations were disturbed. Conclusions Our data demonstrates that loss of Dnmt3b results in derepression of DUX4 in lymphoid tissues and mESCs but not in myogenic cells of D4Z4-2.5/Dnmt3bMommeD14 mice. In addition, the Smchd1MommeD1 variant seems to have a more potent role in DUX4 derepression. Our studies suggest that the immune system is particularly but differentially sensitive to D4Z4 chromatin modifiers which may provide a molecular basis for the yet underexplored immune involvement in FSHD.

Differentiation of Sclerotinia minor depends on thiol redox state and oxidative stress

2008 ◽  
Vol 54 (1) ◽  
pp. 28-36 ◽  
Author(s):  
Nikolaos Patsoukis ◽  
Christos D. Georgiou
Keyword(s):  
Oxidative Stress ◽  
Redox State ◽  
Phytopathogenic Fungus ◽  
Sclerotinia Minor ◽  
Thiol Redox State ◽  
Sh Group ◽  
Thiol Redox ◽  
Sclerotial Differentiation ◽  
And Oxidative Stress

Sclerotial differentiation in Sclerotinia minor is associated with oxidative stress and thiol redox state. The significance of oxidative stress to sclerotial differentiation was revealed by the higher oxidative stress of S. minor compared with a nonsclerotiogenic counterpart. The effect of thiol redox state on sclerotial differentiation was shown by the antioxidant action of the thiol (-SH) group of N-acetylcysteine and cysteine and by an unknown (not antioxidant) role of glutathione (GSH) on S. minor. The nonantioxidant role of GSH was indicated by the differentiation-inhibiting and differentiation-noninhibiting actions of the GSH biosynthesis inhibitor l-buthionine-S,R-sulfoximine and the GSH biosynthesis inducer l-2-oxo-thiazolidine-4-carboxylate, respectively, and by the increase of oxidative stress they caused during the transition from the undifferentiated to differentiated state of S. minor. Moreover, N-acetylcysteine can be used as a potent nontoxic fungicide against this phytopathogenic fungus by acting as a growth-inhibiting cytotoxic oxidant and by sustaining the fungus in the undifferentiated hyphal stage, which is vulnerable to degradation by soil microorganisms.

scholarly journals Increased hepatic telomerase activity in a rat model of iron overload: A role for altered thiol redox state?

2007 ◽  
Vol 42 (2) ◽  
pp. 228-235 ◽  
Author(s):  
Kyle E. Brown ◽  
M. Meleah Mathahs ◽  
Kimberly A. Broadhurst ◽  
Mitchell C. Coleman ◽  
Lisa A. Ridnour ◽  
...  
Keyword(s):  
Iron Overload ◽  
Rat Model ◽  
Redox State ◽  
Telomerase Activity ◽  
Thiol Redox State ◽  
Thiol Redox

scholarly journals Molecular and Clinical Characterization of LAG3 in Breast Cancer Through 2994 Samples

2020 ◽  
Author(s):  
Qiang Liu ◽  
Yihang Qi ◽  
Jie Zhai ◽  
Xiangyi Kong ◽  
Xiangyu Wang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Immune Response ◽  
Cancer Immunotherapy ◽  
Cancer Patients ◽  
Immune Cell ◽  
Immune Checkpoints ◽  
Cell Populations ◽  
Potential Biomarker ◽  
The Impact

Abstract Background Despite the promising impact of cancer immunotherapy targeting CTLA4 and PD1/PDL1, a large number of cancer patients fail to respond. LAG3 (Lymphocyte Activating 3), also named CD233, is a protein Coding gene served as alternative inhibitory receptors to be targeted in the clinic. The impact of LAG3 on immune cell populations and co-regulation of immune response in breast cancer remained largely unknown. Methods To characterize the role of LAG3 in breast cancer, we investigated transcriptome data and associated clinical information derived from a total of 2994 breast cancer patients. Results We observed that LAG3 was closely correlated with major molecular and clinical characteristics, and was more likely to be enriched in higher malignant subtype, suggesting LAG3 was a potential biomarker of triple-negative breast cancer. Furthermore, we estimated the landscape of relationship between LAG3 and ten types of cell populations in breast cancer. Gene ontology analysis revealed LAG3 were strongly correlated with immune response and inflammatory activities. We investigated the correlation pattern between LAG3 and immune modulators in pan-cancer, especially the synergistic role of LAG3 with other immune checkpoints members in breast cancer. Conclusions LAG3 expression was closely related to malignancy of breast cancer and might serve as a potential biomarker; LAG3 might plays an important role in regulating tumor immune microenvironment, not only T cells, but also other immune cells. More importantly, LAG3 might synergize with CTLA4, PD1/ PDL1 and other immune checkpoints, thereby lending more evidences to combination cancer immunotherapy by targeting LAG3, PD1/PDL1, and CTLA4 together.

The modulation of thiol redox state affects the production and metabolism of hydrogen peroxide by heart mitochondria

2005 ◽  
Vol 441 (2) ◽  
pp. 112-122 ◽  
Author(s):  
Maria Pia Rigobello ◽  
Alessandra Folda ◽  
Guido Scutari ◽  
Alberto Bindoli
Keyword(s):  
Hydrogen Peroxide ◽  
Redox State ◽  
Heart Mitochondria ◽  
Thiol Redox State ◽  
Thiol Redox
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