scholarly journals Cover Picture: The cover image features a frozen section from an embryonic day 16 mouse spleen stained by immunofluorescence to demonstrate the presence and location of lymphoid tissue inducer (LTi) cells. Witherset al. (pp. 3240–3245) show that these cells form the focus of lymphocyte accumulation in the developing mouse spleen. Red staining for CD45 marks haemopoietic cells, green shows IL-7Rα expression, and CD4 expression is in blue. LTi cells that express all markers (in white) cluster around blood vessels in the embryonic spleen where signalling to stromal cells initiates lymphocyte recruitment. – Eur. J. Immunol. 11/2007

2007 ◽  
Vol 37 (11) ◽  
pp. NA-NA
Author(s):  
David R. Withers ◽  
Mi-Yeon Kim ◽  
Vasileios Bekiaris ◽  
Simona W. Rossi ◽  
William E. Jenkinson ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Stromal Cells ◽  
Lymphoid Tissue ◽  
Frozen Section ◽  
Image Features ◽  
Cover Image ◽  
Mouse Spleen ◽  
Haemopoietic Cells ◽  
Lymphoid Tissue Inducer

scholarly journals Splenic stromal cells mediate IL-7 independent adult lymphoid tissue inducer cell survival

2010 ◽  
Vol 40 (2) ◽  
pp. 359-365 ◽  
Author(s):  
Tie Zheng Hou ◽  
Mohammad Z. Mustafa ◽  
Sarah J. Flavell ◽  
Fern Barrington ◽  
Eric J. Jenkinson ◽  
...  
Keyword(s):  
Cell Survival ◽  
Stromal Cells ◽  
Lymphoid Tissue ◽  
Lymphoid Tissue Inducer Cell ◽  
Lymphoid Tissue Inducer

scholarly journals Morphofunctional and immunohistochemical characteristics of the large omentum in ovarian cancer

2020 ◽  
Vol 9 (3) ◽  
pp. 64-71
Author(s):  
N. N. Shevlyuk ◽  
L. V. Khalikova ◽  
A. A. Khalikov
Keyword(s):  
Ovarian Cancer ◽  
Connective Tissue ◽  
Protein Expression ◽  
Blood Vessels ◽  
Lymphoid Tissue ◽  
P53 Protein ◽  
Individual Variability ◽  
The Body ◽  
Great Omentum ◽  
Low Grade

The aim of the study was to establish morphofunctional and immunohistochemical characteristics of large omentum in women with ovarian cancer.Material and methods. The large omenta of 48 women with ovarian cancer (low-grade differentiated seropapillary adenocarcinoma of high-grade malignancy) of II stage (n=20) and III stage (n=28) were studied. Histological sections were stained with overview histological and immunohistochemical methods (to reveal ki67, P53, CD34, CD7, CD4, CD8, CD61 proteins expression). Results. In patients, the size of the large omentum was characterized by high individual variability; in the presence of metastasis, the size of the omentum was reduced. Intensive development of blood vessels in the organ was noted, but in the presence of metastases stasis of blood corpuscles, leucocytic infiltration, and moderate edema of connective tissue were observed in the organ’s vessels. Areas of lymphoid tissue, both small lymphatic follicles and diffusely located lymphoid tissue, were revealed in the omentum. In most follicles, reactive centers were not marked, and the number of follicles was reduced in the presence of metastases in the omentum. The analysis of CD34+ cells distribution showed that they were identified both in the tumor and in the areas of the omentum adjacent to the tumor, which indicates a pronounced angiogenesis. An irregular distribution of CD7+ and CD8+ and CD4+ cells was revealed in the tumor tissues, as well as in the surroundings. Simultaneously with the expression of P53 protein, ki67 protein expression is revealed in the significant number of tumor cells (including endothelial cells of tumor blood vessels). The proportion of ki67+ cells in the tumor cell population was 60.1±3.3%. The presence of a large number of ki67+cells in the presence of P53 protein expression in them indicates the aggressiveness of the tumor, as well as a disturbance of apoptosis regulatory mechanisms in the cells. Ki67 expression was low in the omentum areas unaffected by metastases, and it was revealed in the certain areas of connective tissue in fibroblastic programmed differentiation cells. Conclusion. The results obtained indicate significant plasticity and reactivity of great omentum in the presence of tumor process in the body and confirm the important role of great omentum in protective reactions.

Lymphoid tissue inducer cells in adaptive CD4 T cell dependent responses

2008 ◽  
Vol 20 (3) ◽  
pp. 159-163 ◽  
Author(s):  
Peter Lane ◽  
Mi-Yeon Kim ◽  
David Withers ◽  
Fabrina Gaspal ◽  
Vasileios Bekiaris ◽  
...  
Keyword(s):  
T Cell ◽  
Lymphoid Tissue ◽  
Cd4 T Cell ◽  
Lymphoid Tissue Inducer Cells ◽  
Lymphoid Tissue Inducer

Abstract 31: First Trimester Human Umbilical Cord Perivascular Cells (FTM HUCPVCs) Show Superior Angiogenic Potential in Angiogenic Potency Assays When Compared to Term HUCPVCs and Bone Marrow Stromal Cells

2016 ◽  
Vol 119 (suppl_1) ◽  
Author(s):  
Farwah Iqbal ◽  
Peter Szaraz ◽  
Jun Wu ◽  
Andree Gauthier-Fisher ◽  
Ren-Ke Li ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Umbilical Cord ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Aortic Ring ◽  
First Trimester ◽  
Marrow Stromal Cells ◽  
Human Umbilical Cord ◽  
Angiogenic Potential ◽  
Aortic Ring Assay

Introduction: Cell therapy employing pro-angiogenic cell types is a promising option for promoting revascularization of ischemic tissues. First trimester human umbilical cord perivascular cells (FTM HUCPVCs) are a young source of mesenchymal stromal cells (MSCs) that support blood vessels in the umbilical cord. Objective: We aimed to determine the angiogenic potential of FTM HUCPVCs using angiogenic potency assays and compare to older sources of MSCs: term HUCPVCs and bone marrow stromal cells (BMSCs). Methods: For the aortic ring assay, aortas were sectioned and embedded into Matrigel™. Fluorophore-labeled MSCs for testing were added to developing endothelial networks (Day0). MSC integration and network development were monitored by microscopy and quantification of endothelial networks was performed using ImageJ™ software (Day4) n=3. Using the Matrigel™ plug assay, 5.0 x10 5 MSCs were suspended with equal volumes of Matrigel™ and injected subcutaneously in 11-week-old nude mice and isolated after two weeks. Plug associated microvasculature was imaged and quantified n=3. Results: In the aortic ring assay, FTM HUCPVCs homed to endothelial networks and demonstrated greater endothelial cell coverage, when compared to term HUCPVCs and BMSCs. FTM HUCPVCs showed significantly greater network growth when compared to term HUCPVCs ( p ≤0.001), BMSCs ( p ≤0.001) and untreated endothelial networks ( p ≤0.001). FTM HUCPVC contributed to a significantly greater number of closed loops when compared to term HUCPVCs ( p ≤0.01), BMSCs ( p ≤0.001) and untreated networks ( p ≤0.05). At two weeks following injection of Matrigel plugs, FTM HUCPVC resulted in significantly greater blood vessel recruitment when compared to term HUCPVCs ( p ≤0.05), BMSCs ( p ≤0.01) and control media plugs ( p ≤0.01). Small tortuous blood vessels were found in significantly higher quantity in FTM HUCPVC injected plugs when compared to term HUCPVCs ( p ≤0.05), BMSCs (p ≤0.01) and media plugs ( p ≤0.001). Conclusions: These studies demonstrate that FTM HUCPVCs have superior potential to augment, both the initiation of capillary formation and the development of functional, perfusable blood vessels, highlighting their potential for tissue regeneration following ischemic injury.

scholarly journals Runx1/Cbfβ2 Complexes Are Required for Lymphoid Tissue Inducer Cell Differentiation at Two Developmental Stages

2010 ◽  
Vol 186 (3) ◽  
pp. 1450-1457 ◽  
Author(s):  
Masashi Tachibana ◽  
Mari Tenno ◽  
Chieko Tezuka ◽  
Machiko Sugiyama ◽  
Hisahiro Yoshida ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Lymphoid Tissue ◽  
Developmental Stages ◽  
Lymphoid Tissue Inducer Cell ◽  
Lymphoid Tissue Inducer

scholarly journals Mesenchymal Stromal-Like Cells in the Glioma Microenvironment: What Are These Cells?

Cancers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2628
Author(s):  
Anne Clavreul ◽  
Philippe Menei
Keyword(s):  
Blood Vessels ◽  
Immune Cells ◽  
Stromal Cells ◽  
Low Ph ◽  
Cancer Associated Fibroblasts ◽  
Tumor Resistance ◽  
Interstitial Pressure ◽  
Close Proximity ◽  
Cellular Components ◽  
Glioma Microenvironment

The glioma microenvironment is a critical regulator of tumor progression. It contains different cellular components such as blood vessels, immune cells, and neuroglial cells. It also contains non-cellular components, such as the extracellular matrix, extracellular vesicles, and cytokines, and has certain physicochemical properties, such as low pH, hypoxia, elevated interstitial pressure, and impaired perfusion. This review focuses on a particular type of cells recently identified in the glioma microenvironment: glioma-associated stromal cells (GASCs). This is just one of a number of names given to these mesenchymal stromal-like cells, which have phenotypic and functional properties similar to those of mesenchymal stem cells and cancer-associated fibroblasts. Their close proximity to blood vessels may provide a permissive environment, facilitating angiogenesis, invasion, and tumor growth. Additional studies are required to characterize these cells further and to analyze their role in tumor resistance and recurrence.

scholarly journals Human innate lymphoid cell precursors express CD48 that modulates ILC differentiation through 2B4 signaling

2020 ◽  
Vol 5 (53) ◽  
pp. eaay4218
Author(s):  
Dejene M. Tufa ◽  
Ashley M. Yingst ◽  
George Devon Trahan ◽  
Tyler Shank ◽  
Dallas Jones ◽  
...  
Keyword(s):  
Lymphoid Tissue ◽  
Nk Cell ◽  
Developmental Trajectories ◽  
Lymphoid Cells ◽  
Surface Receptors ◽  
Distinct Subset ◽  
The Common ◽  
Lymphoid Tissue Inducer

Innate lymphoid cells (ILCs) develop from common lymphoid progenitors (CLPs), which further differentiate into the common ILC progenitor (CILP) that can give rise to both ILCs and natural killer (NK) cells. Murine ILC intermediates have recently been characterized, but the human counterparts and their developmental trajectories have not yet been identified, largely due to the lack of homologous surface receptors in both organisms. Here, we show that human CILPs (CD34+CD117+α4β7+Lin−) acquire CD48 and CD52, which define NK progenitors (NKPs) and ILC precursors (ILCPs). Two distinct NK cell subsets were generated in vitro from CD34+CD117+α4β7+Lin−CD48−CD52+ and CD34+CD117+α4β7+Lin−CD48+CD52+ NKPs, respectively. Independent of NKPs, ILCPs exist in the CD34+CD117+α4β7+Lin−CD48+CD52+ subset and give rise to ILC1s, ILC2s, and NCR+ ILC3s, whereas CD34+CD117+α4β7+Lin−CD48+CD52− ILCPs give rise to a distinct subset of ILC3s that have lymphoid tissue inducer (LTi)–like properties. In addition, CD48-expressing CD34+CD117+α4β7+Lin− precursors give rise to tissue-associated ILCs in vivo. We also observed that the interaction of 2B4 with CD48 induced differentiation of ILC2s, and together, these findings show that expression of CD48 by human ILCPs modulates ILC differentiation.

IL-12 initiates tumor rejection via lymphoid tissue–inducer cells bearing the natural cytotoxicity receptor NKp46

2010 ◽  
Vol 11 (11) ◽  
pp. 1030-1038 ◽  
Author(s):  
Maya Eisenring ◽  
Johannes vom Berg ◽  
Glen Kristiansen ◽  
Elisabeth Saller ◽  
Burkhard Becher
Keyword(s):  
Lymphoid Tissue ◽  
Tumor Rejection ◽  
Natural Cytotoxicity ◽  
Natural Cytotoxicity Receptor ◽  
Lymphoid Tissue Inducer Cells ◽  
Lymphoid Tissue Inducer
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