scholarly journals Influenza A virus abrogates IFN-γ response in respiratory epithelial cells by disruption of the Jak/Stat pathway

2008 ◽  
Vol 38 (6) ◽  
pp. 1559-1573 ◽  
Author(s):  
Kohsaku Uetani ◽  
Miki Hiroi ◽  
Tadamichi Meguro ◽  
Hiroshi Ogawa ◽  
Toshinori Kamisako ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Influenza A Virus ◽  
Influenza A ◽  
Respiratory Epithelial Cells ◽  
Ifn Γ ◽  
Respiratory Epithelial ◽  
Stat Pathway

Hypothiocyanite produced by human and rat respiratory epithelial cells inactivates extracellular H1N2 influenza A virus

2015 ◽  
Vol 65 (1) ◽  
pp. 71-80 ◽  
Author(s):  
Aaron Gingerich ◽  
Lan Pang ◽  
Jarod Hanson ◽  
Daniel Dlugolenski ◽  
Rebecca Streich ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Influenza A Virus ◽  
Influenza A ◽  
Respiratory Epithelial Cells ◽  
Respiratory Epithelial

scholarly journals Influenza A Virus Protein PB1-F2 Exacerbates IFN-β Expression of Human Respiratory Epithelial Cells

2010 ◽  
Vol 185 (8) ◽  
pp. 4812-4823 ◽  
Author(s):  
Ronan Le Goffic ◽  
Edwige Bouguyon ◽  
Christophe Chevalier ◽  
Jasmina Vidic ◽  
Bruno Da Costa ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Influenza A Virus ◽  
Influenza A ◽  
Virus Protein ◽  
Respiratory Epithelial Cells ◽  
Human Respiratory Epithelial Cells ◽  
Respiratory Epithelial

scholarly journals Host-directed combinatorial RNAi improves inhibition of diverse strains of influenza A virus in human respiratory epithelial cells

PLoS ONE ◽  
2018 ◽  
Vol 13 (5) ◽  
pp. e0197246 ◽  
Author(s):  
Michael A. Estrin ◽  
Islam T. M. Hussein ◽  
Wendy B. Puryear ◽  
Anne C. Kuan ◽  
Stephen C. Artim ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Influenza A Virus ◽  
Influenza A ◽  
Respiratory Epithelial Cells ◽  
Human Respiratory Epithelial Cells ◽  
Respiratory Epithelial

scholarly journals Glycan Analysis and Influenza A Virus Infection of Primary Swine Respiratory Epithelial Cells

2010 ◽  
Vol 285 (44) ◽  
pp. 34016-34026 ◽  
Author(s):  
Allen C. Bateman ◽  
Rositsa Karamanska ◽  
Marc G. Busch ◽  
Anne Dell ◽  
Christopher W. Olsen ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Virus Infection ◽  
Influenza A Virus ◽  
Influenza A ◽  
Respiratory Epithelial Cells ◽  
Glycan Analysis ◽  
Influenza A Virus Infection ◽  
Respiratory Epithelial

Evaluation of infectivity of a canine lineage H3N8 influenza A virus in ponies and in primary equine respiratory epithelial cells

2011 ◽  
Vol 72 (8) ◽  
pp. 1071-1078 ◽  
Author(s):  
Ayshea M. Quintana ◽  
Stephen B. Hussey ◽  
Ema C. Burr ◽  
Heidi L. Pecoraro ◽  
Kristina M. Annis ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Influenza A Virus ◽  
Influenza A ◽  
Respiratory Epithelial Cells ◽  
Respiratory Epithelial

scholarly journals Estrogenic compounds reduce influenza A virus replication in primary human nasal epithelial cells derived from female, but not male, donors

2016 ◽  
Vol 310 (5) ◽  
pp. L415-L425 ◽  
Author(s):  
Jackye Peretz ◽  
Andrew Pekosz ◽  
Andrew P. Lane ◽  
Sabra L. Klein
Keyword(s):  
Estrogen Receptor ◽  
Epithelial Cells ◽  
Influenza A Virus ◽  
Virus Replication ◽  
Influenza A ◽  
Molecular Mechanisms ◽  
Acute Infection ◽  
Respiratory Epithelial Cells ◽  
Human Nasal Epithelial Cells ◽  
Respiratory Epithelial

Influenza causes an acute infection characterized by virus replication in respiratory epithelial cells. The severity of influenza and other respiratory diseases changes over the life course and during pregnancy in women, suggesting that sex steroid hormones, such as estrogens, may be involved. Using primary, differentiated human nasal epithelial cell (hNEC) cultures from adult male and female donors, we exposed cultures to the endogenous 17β-estradiol (E2) or select estrogen receptor modulators (SERMs) and then infected cultures with a seasonal influenza A virus (IAV) to determine whether estrogenic signaling could affect the outcome of IAV infection and whether these effects were sex dependent. Estradiol, raloxifene, and bisphenol A decreased IAV titers in hNECs from female, but not male, donors. The estrogenic decrease in viral titer was dependent on the genomic estrogen receptor-2 (ESR2) as neither genomic ESR1 nor nongenomic GPR30 was expressed in hNEC cultures and addition of the genomic ER antagonist ICI 182,780 reversed the antiviral effects of E2. Treatment of hNECs with E2 had no effect on interferon or chemokine secretion but significantly downregulated cell metabolic processes, including genes that encode for zinc finger proteins, many of which contain estrogen response elements in their promoters. These data provide novel insights into the cellular and molecular mechanisms of how natural and synthetic estrogens impact IAV infection in respiratory epithelial cells derived from humans.

scholarly journals Primary Swine Respiratory Epithelial Cell Lines for the Efficient Isolation and Propagation of Influenza A Viruses

2020 ◽  
Vol 94 (24) ◽  
Author(s):  
Victoria Meliopoulos ◽  
Sean Cherry ◽  
Nicholas Wohlgemuth ◽  
Rebekah Honce ◽  
Karen Barnard ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Epithelial Cells ◽  
Cell Lines ◽  
Influenza A ◽  
Vaccine Development ◽  
Mdck Cells ◽  
Influenza Viruses ◽  
Clinical Samples ◽  
Respiratory Epithelial Cells ◽  
Respiratory Epithelial

ABSTRACT Influenza virus isolation from clinical samples is critical for the identification and characterization of circulating and emerging viruses. Yet efficient isolation can be difficult. In these studies, we isolated primary swine nasal and tracheal respiratory epithelial cells and immortalized swine nasal epithelial cells (siNEC) and tracheal epithelial cells (siTEC) that retained the abilities to form tight junctions and cilia and to differentiate at the air-liquid interface like primary cells. Critically, both human and swine influenza viruses replicated in the immortalized cells, which generally yielded higher-titer viral isolates from human and swine nasal swabs, supported the replication of isolates that failed to grow in Madin-Darby canine kidney (MDCK) cells, and resulted in fewer dominating mutations during viral passaging than MDCK cells. IMPORTANCE Robust in vitro culture systems for influenza virus are critically needed. MDCK cells, the most widely used cell line for influenza isolation and propagation, do not adequately model the respiratory tract. Therefore, many clinical isolates, both animal and human, are unable to be isolated and characterized, limiting our understanding of currently circulating influenza viruses. We have developed immortalized swine respiratory epithelial cells that retain the ability to differentiate and can support influenza replication and isolation. These cell lines can be used as additional tools to enhance influenza research and vaccine development.

Sign in / Sign up

Export Citation Format

Share Document