scholarly journals CD40-dependent and -independent activation of human tonsil B cells by CpG oligodeoxynucleotides

2003 ◽  
Vol 33 (6) ◽  
pp. 1576-1585 ◽  
Author(s):  
Florian Gantner ◽  
Patrice Hermann ◽  
Kosuke Nakashima ◽  
Satoko Matsukawa ◽  
Katsuya Sakai ◽  
...  
Keyword(s):  
B Cells ◽  
Human Tonsil ◽  
Cpg Oligodeoxynucleotides

Circulating CD24hiCD38hi regulatory B cells correlate inversely with the ThEM17 cell frequency in granulomatosis with polyangiitis patients

Rheumatology ◽  
2019 ◽  
Vol 58 (8) ◽  
pp. 1361-1366 ◽  
Author(s):  
Anouk von Borstel ◽  
Lucas L Lintermans ◽  
Peter Heeringa ◽  
Abraham Rutgers ◽  
Coen A Stegeman ◽  
...  
Keyword(s):  
B Cells ◽  
Granulomatosis With Polyangiitis ◽  
Cultured Cells ◽  
Effector Memory ◽  
Regulatory B Cells ◽  
Cell Frequency ◽  
Th1 Cell ◽  
Th Cells ◽  
Cpg Oligodeoxynucleotides

Abstract Objectives To investigate whether there is a direct relation between expanded proportions of Th17 effector memory (ThEM17) cells and regulatory B cells (Bregs) in peripheral blood of granulomatosis with polyangiitis (GPA) patients. Methods Frequencies of Bregs and ThEM17 cells, as well as ThEM1 cells, were determined by flow cytometry in blood samples from 42 GPA patients in remission and 18 matched healthy controls (HCs). The Breg frequency was defined as CD24hiCD38hiCD19+ cells. ThEM17 cells were defined as CCR6+CXCR3-CCR4+ cells and ThEM1 cells as CCR6-CXCR3+CCR4- cells within the CD3+CD4+CD45RO+CCR7- population. In addition, CD3+CD4+ Th cells from 9 GPA patients were co-cultured in vitro with either total B cells or a Breg-depleted B cell fraction. Cultured cells were stimulated with Staphylococcus Enterotoxin B (SEB) and CpG-oligodeoxynucleotides (CpG-ODN). Th17- (IL-17+) and Th1 cell (IFNγ+) frequencies were determined at baseline and day 5 upon restimulation with phorbol myristate acetate (PMA) and Ca-I. Results A decreased Breg frequency was found in treated GPA patients, whereas an increased ThEM17 cell frequency was observed in treated and untreated GPA patients compared with HCs. Additionally, a decreased ThEM1 cell frequency was seen in untreated GPA patients compared with HCs. In untreated GPA patients circulating Breg frequencies correlated negatively with ThEM17 cells (r = −0.533; P = 0.007) and positively with ThEM1 cells (r = −0.473; P = 0.015). The co-culture experiments revealed a significant increase in the frequency of IL-17+ Th cells in Breg-depleted samples (median: 3%; range: 1–7.5%) compared with Breg-undepleted samples (P = 0.002; undepleted samples median: 2.1%; range: 0.9–6.4%), whereas no difference in the frequency of IFNγ+ Th cells in Breg-depleted cultures was observed (undepleted median: 11.8%; range: 2.8–21% vs Breg-depleted median: 12.2%; range: 2.6–17.6%). Conclusion Bregs modulate ThEM17 responses in GPA patients. Future studies should elaborate on clinical and therapeutical implications of the Breg-Th17 interaction in GPA patients.

scholarly journals CpG-oligodeoxynucleotides stimulate immunoglobulin A secretion in intestinal mucosal B cells

2009 ◽  
Vol 155 (3) ◽  
pp. 534-540 ◽  
Author(s):  
S. H. Blaas ◽  
M. Stieber-Gunckel ◽  
W. Falk ◽  
F. Obermeier ◽  
G. Rogler
Keyword(s):  
B Cells ◽  
Immunoglobulin A ◽  
Cpg Oligodeoxynucleotides

CpG oligodeoxynucleotides modulate innate and adaptive functions of IGM+ B cells in rainbow trout

2019 ◽  
Vol 91 ◽  
pp. 396
Author(s):  
R. Simón ◽  
P. Díaz-Rosales ◽  
E. Morel ◽  
A.G. Granja ◽  
C. Tafalla
Keyword(s):  
Rainbow Trout ◽  
B Cells ◽  
Cpg Oligodeoxynucleotides

scholarly journals B Cells Capturing Antigen Conjugated with CpG Oligodeoxynucleotides Induce Th1 Cells by Elaborating IL-12

2002 ◽  
Vol 169 (2) ◽  
pp. 787-794 ◽  
Author(s):  
Hidekazu Shirota ◽  
Kunio Sano ◽  
Noriyasu Hirasawa ◽  
Tadashi Terui ◽  
Kazuo Ohuchi ◽  
...  
Keyword(s):  
B Cells ◽  
Th1 Cells ◽  
Cpg Oligodeoxynucleotides

CpG oligodeoxynucleotides stimulate IFN-γ-inducible protein-10 production in human B cells

2004 ◽  
Vol 10 (6) ◽  
pp. 431-438 ◽  
Author(s):  
Jörg Vollmer ◽  
Marion Jurk ◽  
Ulrike Samulowitz ◽  
Grayson Lipford ◽  
Alexandra Forsbach ◽  
...  
Keyword(s):  
B Cells ◽  
Cpg Oligodeoxynucleotides ◽  
Human B Cells ◽  
Ifn Γ ◽  
Inducible Protein

CpG oligodeoxynucleotides directly induce CXCR3 chemokines in human B cells

2004 ◽  
Vol 320 (4) ◽  
pp. 1139-1147 ◽  
Author(s):  
Atsushi Kato ◽  
Takahisa Ogasawara ◽  
Toshiki Homma ◽  
Jonathan Batchelor ◽  
Shosuke Imai ◽  
...  
Keyword(s):  
B Cells ◽  
Cpg Oligodeoxynucleotides ◽  
Human B Cells

CpG oligodeoxynucleotides discriminately enhance binding capacity of human naïve B cells to Hepatitis B virus epitopes

2012 ◽  
Vol 58 (6) ◽  
pp. 752-759 ◽  
Author(s):  
Jian-ying Bai ◽  
Yong-tao Yang ◽  
Rong Zhu ◽  
Yi-qin Wang ◽  
Yin Tian ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
B Cells ◽  
Hepatitis B ◽  
Binding Capacity ◽  
Class Ii ◽  
Class I ◽  
Cpg Odn ◽  
Cpg Oligodeoxynucleotides ◽  
Class Ii Mhc ◽  
B Virus

CpG oligodeoxynucleotides (CpG ODN) have the potential to enhance the antigen-presenting cells function of human naïve B cells. In this study, we aim to define the effect of CpG ODNs on the binding capacity of human naïve B cells for different Hepatitis B virus (HBV) epitopes. Three HLA-A2 restricted epitopes were selected to incubate with CpG ODN-primed human naïve B cells. Binding capacity for each epitope and expression of CD80, CD86, class I major histocompatibility complex (MHC), and class II MHC of naïve B cells was tested, respectively, by flow cytometry. CpG ODNs, especially ODN 2216, enhanced the binding capacity of human naïve B cells for HBV epitopes (p < 0.01), and induced markedly higher expression of CD80, CD86, class I MHC, and class II MHC. The binding capacity of CpG-treated naive B cells for each epitope was significantly different. In all the 3 subjects, CpG ODN 2216-primed naïve B cells showed the highest binding ability for Env172–180 compared with the other epitopes with a high expression of co-stimulatory and MHC molecules. CpG ODN showed the potential to selectively enhance the binding capacity of human naïve B cells for HBV epitopes. These results suggest new strategies for development of vaccine design.

scholarly journals Potential Immunological Adjuvant of `K'-Type CpG-Oligodeoxynucleotides Enhanced the Cell Proliferation and IL-6 mRNA Transcription in Canine B Cells

2011 ◽  
Vol 73 (2) ◽  
pp. 177-184 ◽  
Author(s):  
Shinpei KAWARAI ◽  
Kaoru SATO ◽  
Anna HORIGUCHI ◽  
Keigo KURATA ◽  
Akio KIUCHI ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
B Cells ◽  
Mrna Transcription ◽  
Cpg Oligodeoxynucleotides
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