Biased expression of individual T cell receptor V gene segments in CD4+ and CD8+ human peripheral blood T lymphocytes

1991 ◽  
Vol 21 (3) ◽  
pp. 819-822 ◽  
Author(s):  
Johan Grunewald ◽  
Carl Harald Janson ◽  
Hans Wigzell
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
T Cell Receptor ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Cell Receptor ◽  
V Gene

scholarly journals Peripheral CD25 positive T lymphocytes with biased T cell receptor V  gene usage in autoimmune endogenous posterior uveitis

1995 ◽  
Vol 48 (1) ◽  
pp. M46-M50 ◽  
Author(s):  
P J Tighe ◽  
J V Forrester ◽  
J Liversidge ◽  
H F Sewell
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Posterior Uveitis ◽  
Gene Usage ◽  
V Gene

scholarly journals High Frequency of Cancer Patients with Abnormal Assembly of the T Cell Receptor-CD3 Complex in Peripheral Blood T Lymphocytes

1994 ◽  
Vol 85 (12) ◽  
pp. 1189-1192 ◽  
Author(s):  
Yoshio Gunji ◽  
Seiji Hori ◽  
Tomohiko Aoe ◽  
Takehide Asano ◽  
Takenori Ochiai ◽  
...  
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
Cancer Patients ◽  
T Cell Receptor ◽  
Peripheral Blood ◽  
High Frequency ◽  
Cell Receptor

scholarly journals Characterization of CD3+, CD4-, CD8- clones expressing the putative T cell receptor gamma gene product. Analysis of the activation pathways leading to interleukin 2 production and triggering of the lytic machinery.

1987 ◽  
Vol 166 (1) ◽  
pp. 277-282 ◽  
Author(s):  
S Ferrini ◽  
C Bottino ◽  
R Biassoni ◽  
A Poggi ◽  
R P Sekaly ◽  
...  
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
T Cell Receptor ◽  
Cell Activation ◽  
Human Peripheral Blood ◽  
Interleukin 2 ◽  
Cell Receptor ◽  
Target Cells ◽  
Product Analysis ◽  
Alpha Beta

Four clones were derived from human peripheral blood T lymphocytes from which CD4+ and CD8+ cells had been removed by treatment with specific mAbs and complement. All expressed the CD2+, 3+, 4-, 8-, T44- phenotype, and did not react with the WT31 mAb, which is specific for a framework determinant of the CD3-associated alpha/beta heterodimer which serves as receptor for antigen on most human T lymphocytes. Surface iodination followed by crosslinking with dithiobis-succinimidyl propionate (DSP) and immunoprecipitation with anti-CD3 mAbs indicated that, in all four clones, the CD3-associated molecules consisted of a major 45 kD band and a minor band of 43 kD. Northern blot analysis showed that mRNA for the gamma chain was expressed at high levels, whereas mRNA for the alpha chain was missing; beta chain mRNA was present in a defective form (1 kb instead of 1.3 kb). These data support the concept that these clones may express, in association with CD3, the molecular product of the T cell receptor gamma genes instead of the typical alpha/beta heterodimer. CD3+, WT31- clones lysed the NK-sensitive K562 target cells and produced IL-2 upon stimulation with PHA. In addition, they released IL-2 after triggering with soluble anti-CD3 mAbs or with an appropriate combination of anti-CD2 mAbs (in the presence of adherent cells). When CD3+, WT31- clones were incubated with an anti-CD3 producing hybridoma as triggering target, the latter was efficiently lysed. Target cell lysis also occurred when a suitable combination of anti-CD2 mAbs-producing hybridomas was used. Therefore, CD3+, WT31- cells appear to use two pathways of cell activation that function also in conventional CD3+, WT31+ T cells, but they lack a third putative pathway initiated by T44 surface molecules.

Two forms of the T-cell receptor γ protein found on peripheral blood cytotoxic T lymphocytes

Nature ◽  
1987 ◽  
Vol 325 (6106) ◽  
pp. 689-694 ◽  
Author(s):  
Michael B. Brenner ◽  
Joanne McLean ◽  
Harriet Scheft ◽  
Janice Riberdy ◽  
Siew-Lan Ang ◽  
...  
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
T Cell Receptor ◽  
Cytotoxic T Lymphocytes ◽  
Peripheral Blood ◽  
Cell Receptor

scholarly journals Expansion of a unique subpopulation of cytotoxic T cells that express a C alpha V delta 1 T-cell receptor gene in a patient with severe persistent neutropenia

Blood ◽  
1992 ◽  
Vol 80 (12) ◽  
pp. 3157-3163
Author(s):  
I Bank ◽  
M Book ◽  
L Cohen ◽  
A Kneller ◽  
E Rosental ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Lymphocytes ◽  
T Cell Receptor ◽  
T Lymphocyte ◽  
Peripheral Blood ◽  
Gene Segment ◽  
Cell Receptor ◽  
Severe Neutropenia ◽  
Pcr Analysis

CD8+ T-lymphocyte populations may be expanded in the peripheral blood of patients with chronic idiopathic neutropenia and may be involved in suppression of granulopoiesis. In this report, we have analyzed the T-cell receptor (TCR) used by the T lymphocytes of a patient with chronic severe neutropenia. Using specific oligonucleotides in the polymerase chain reaction (PCR) to amplify cDNA specific for the different families of the V alpha, V beta, and V delta TCR genes, and monoclonal antibodies (MoAbs) to examine T-lymphocyte subsets and their TCR, a persistent expansion of CD3+CD8+ T lymphocytes and a reduced repertoire of TCR V alpha and V beta genes were found in the patient's peripheral blood mononuclear cell (PBMC) preparations. A predominant portion of the T lymphocytes expressed a unique TCR structure. Thus, we found that, despite the fact that 98% of the T cells expressed alpha beta TCR on the surface membrane and less than 2% expressed tau delta TCR, nonetheless, 40% to 60% of the T cells stained positively with anti V delta 1 MoAb. Using the PCR analysis, the V delta 1 gene segment was found to be rearranged to C alpha, rather than to C delta genes. The expanded C alpha V delta 1+ cells, which are found only rarely in normal PB, expressed CD8 and were cytotoxic, and the C alpha V delta 1 receptor was functional in cytotoxicity. This constitutes the first description of an expansion of cytotoxic CD8+ lymphocytes expressing a functional “hybrid” C alpha V delta 1 gene in vivo, and suggests a pathogenic role for CD8+ C alpha V delta 1+ cells in some patients with idiopathic neutropenia.

Isolation of functionally active T cell receptor γδ-bearing lymphocytes from human peripheral blood

1989 ◽  
Vol 118 (2) ◽  
pp. 251-255 ◽  
Author(s):  
Vidar Bosnes ◽  
Ragnhild Halvorsen ◽  
Gustav Gaudernack ◽  
Erik Thorsby
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Cell Receptor
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