Human T cell clones can inducein vitro IgE synthesis in normal B cells regardless of alloantigen recognition or specificity for peculiar antigens

1986 ◽  
Vol 16 (12) ◽  
pp. 1509-1514 ◽  
Author(s):  
Gianfranco Del Prete ◽  
Enrico Maggi ◽  
Donatella Macchia ◽  
Antonio Tiri ◽  
Paola Parronchi ◽  
...  
Keyword(s):  
T Cell ◽  
B Cells ◽  
T Cell Clones ◽  
Human T Cell ◽  
Cell Clones ◽  
Ige Synthesis

539 Interleukin 4 (IL-4) is responsible for IgE synthesis induced in vitro by human T cell clones

1988 ◽  
Vol 81 (1) ◽  
pp. 303 ◽  
Author(s):  
S. Romagnani ◽  
G.F. Del Prete ◽  
E. Maggi ◽  
P. Parronchi ◽  
A. Tiri ◽  
...  
Keyword(s):  
T Cell ◽  
Interleukin 4 ◽  
T Cell Clones ◽  
Human T Cell ◽  
Cell Clones ◽  
Ige Synthesis

scholarly journals The 26-kD transmembrane form of tumor necrosis factor alpha on activated CD4+ T cell clones provides a costimulatory signal for human B cell activation.

1993 ◽  
Vol 177 (6) ◽  
pp. 1575-1585 ◽  
Author(s):  
G Aversa ◽  
J Punnonen ◽  
J E de Vries
Keyword(s):  
T Cells ◽  
T Cell ◽  
B Cells ◽  
Cd4 T Cells ◽  
Plasma Membranes ◽  
Cd4 T Cell ◽  
Tnf Alpha ◽  
T Cell Clones ◽  
Cell Clones ◽  
Ige Synthesis

Interleukin 4 (IL-4) induces immunoglobulin (Ig)E and IgG4 synthesis in human B cells. In addition to IL-4, costimulatory signals provided by activated CD4+ T cells are required for productive IgG4 and IgE synthesis. Here we report that the 26-kD transmembrane form of tumor necrosis factor alpha (mTNF-alpha), which is rapidly expressed on CD4+ T cell clones after activation, contributes to the costimulatory signals resulting in IL-4-dependent Ig synthesis by B cells, including IgG4 and IgE production. mTNF-alpha expression was induced on T cell clones within 2 h after activation with concanavalin A. Peak expression was observed at 24 h, followed by a gradual decrease, but appreciable levels of mTNF-alpha were still detectable 72 h after activation. The presence of the 26-kD membrane form of TNF-alpha on activated T cell clones was confirmed by immunoprecipitation. Monoclonal antibodies (mAbs) recognizing mTNF-alpha, or the p55 TNF receptor, inhibited IgM, IgG, IgG4, and IgE synthesis induced by IL-4 and activated CD4+ T cell clones in cultures of highly purified surface IgD+ B cells. The anti-TNF-alpha mAbs also blocked Ig production in cultures in which the activated CD4+ T cell clones were replaced by their plasma membranes. Furthermore, pretreatment of the plasma membranes with anti-TNF-alpha mAbs strongly reduced their capacity to stimulate B cells to produce Ig in the presence of IL-4, indicating that the anti-TNF-alpha mAbs blocked the effects of mTNF-alpha. Anti-TNF-alpha mAbs did not affect IgM, IgG, IgG4, or IgE synthesis induced by anti-CD40 mAbs and IL-4 in the absence of CD4+ T cells, supporting the notion that the anti-TNF-alpha mAbs indeed interfered with the costimulatory, contact-mediated signal provided by T cells, or their membranes. Collectively these results indicate that mTNF-alpha, which is rapidly induced after activation of CD4+ T cells, participates in productive T-B cell interactions resulting in IL-4-induced Ig production. This is a novel property of the T cell membrane form of TNF-alpha.

scholarly journals Induction of IgE synthesis in normal human B cells. Sequential requirements for activation by an alloreactive T cell clone and IgE-potentiating factors.

1986 ◽  
Vol 163 (3) ◽  
pp. 713-723 ◽  
Author(s):  
D Y Leung ◽  
M C Young ◽  
N Wood ◽  
R S Geha
Keyword(s):  
T Cell ◽  
B Cells ◽  
Cell Clone ◽  
Mixed Lymphocyte Culture ◽  
Lymphocyte Culture ◽  
T Cell Clones ◽  
Cell Clones ◽  
T Cell Clone ◽  
Ige Synthesis ◽  
Alloreactive T Cell

Two human alloreactive T cell clones were established from a one-way mixed lymphocyte culture involving two nonatopic donors, and were assessed for their capacity to induce IgE synthesis by B cells obtained from the original stimulator. The two alloreactive T cell clones studied induced IgG but not IgE synthesis in normal B cells. However, one of the two clones, clone 2H6, induced IgE synthesis in the presence of supernatants from T cell lines derived from patients with the hyper-IgE syndrome (HIE), and enriched for T cells bearing receptors for IgE. These supernatants by themselves caused no IgE synthesis in nonatopic B cells. The potentiating factors in these supernatants were shown to bind to IgE. Time sequence experiments indicated that interaction of the B cells with the alloreactive clone 2H6 renders them responsive to the action of the IgE-potentiating factors. These results indicate that induction of IgE synthesis in normal B cells involves at least two sequential T cell derived signals. Furthermore, T cell clones are heterogenous in their capacity to provide these signals.

scholarly journals T cell clones from an X-linked hyper-immunoglobulin (IgM) patient induce IgE synthesis in vitro despite expression of nonfunctional CD40 ligand.

1994 ◽  
Vol 180 (5) ◽  
pp. 1775-1784 ◽  
Author(s):  
P Life ◽  
J F Gauchat ◽  
V Schnuriger ◽  
S Estoppey ◽  
G Mazzei ◽  
...  
Keyword(s):  
T Cell ◽  
B Cells ◽  
Rnase Protection Assay ◽  
Cd40 Ligand ◽  
Tnf Alpha ◽  
Rnase Protection ◽  
T Cell Clones ◽  
Cell Clones ◽  
Ige Synthesis

The induction of immunoglobulin E (IgE) switching in B cells requires at least two signals. The first is given by either of the soluble lymphokines interleukin 4 (IL-4) or IL-13, whereas the second is contact dependent. It has been widely reported that a second signal can be provided by the CD40 ligand (CD40L) expressed on the surface of T cells, mast cells, and basophils. A defect in the CD40L has been shown recently to be responsible for the lack of IgE, IgA, and IgG, characteristic of the childhood X-linked immunodeficiency, hyper IgM syndrome (HIGM1). IgE can however be detected in the serum of some HIGM1 patients. In this study, we isolated T cell clones and lines using phytohemagglutinin (PHA) and allergen, respectively, from the peripheral blood of one such patient who expressed a truncated form of CD40L, and investigated their ability to induce IgE switching in highly purified, normal tonsillar B cells in vitro. Unexpectedly, 4 of 12 PHA clones tested induced contact-dependent IgE synthesis in the presence of exogenous IL-4. These clones were also shown to strongly upregulated IL-4-induced germline epsilon RNA and formed dense aggregates with B cells. Of the four helper clones, three were CD8+, of which two were characteristic of the T helper cell 2 (Th2) subtype. Two allergen-specific HIGM1 T cell lines, both of the Th0 subtype, could also drive IgE synthesis when prestimulated using specific allergen. All clones and lines were negative for surface expression of CD40L, and the mutated form of CD40L was confirmed for a representative clone by RNase protection assay and sequencing. The IgE helper activity could not be attributed to membrane tumor necrosis factor alpha (TNF-alpha) although it was strongly expressed on activated clones, and the addition of neutralizing anti-TNF-alpha antibody did not abrogate IgE synthesis. These results therefore suggest the involvement of T cell surface molecules other than CD40L in the induction of IgE synthesis, and that these molecules may also be implicated in other aspects of T-B cell interactions.

scholarly journals Differential requirements of B cells from normal and allergic subjects for the induction of IgE synthesis by an alloreactive T cell clone.

1985 ◽  
Vol 162 (1) ◽  
pp. 202-214 ◽  
Author(s):  
D T Umetsu ◽  
D Y Leung ◽  
R Siraganian ◽  
H H Jabara ◽  
R S Geha
Keyword(s):  
T Cells ◽  
T Cell ◽  
B Cells ◽  
Cell Clone ◽  
T Cell Clones ◽  
Igg Synthesis ◽  
Cell Clones ◽  
T Cell Clone ◽  
Ige Synthesis ◽  
Alloreactive T Cell

Human T cell helper/inducer clones were used to induce IgE synthesis in B cells from both allergic and nonallergic donors. An alloreactive T cell clone, activated by recognition of specific HLA-DR antigens, stimulated peripheral blood B cells from both allergic and nonallergic donors to synthesize IgE antibody. B cells of allergic donors differed from those of nonallergic donors in their requirements for induction of IgE synthesis. Induction of IgE synthesis in B cells from nonallergic individuals occurred only under conditions of cognate interaction, in which the B cells expressed the alloantigen recognized by the T cells. In contrast, IgE synthesis in B cells from allergic donors occurred under conditions of cognate interaction with T cells as well as bystander conditions where the B cells did not express the alloantigen recognized by the T cell clones and where the T cell clones were stimulated by third-party monocytes bearing the relevant alloantigens. Furthermore, bystander stimulation of IgE synthesis in allergic donors occurred in the presence of tetanus toxoid (TT) antigen-specific T cell clones activated by the appropriate TT-pulsed monocytes. In contrast to the differing requirements of B cells from normal vs. allergic subjects for the induction of IgE synthesis, these B cells did not differ in their requirements for the induction of IgG synthesis. IgG synthesis was induced in all B cells under conditions of cognate interaction with the T cells as well as under conditions of bystander stimulation. These results suggest that cognate T-B cell interactions may be important in the development of IgE immune responses in the normal host.

Tetanus toxin light chain recognition by CD4+ and CD8+ human T cell clones

1995 ◽  
Vol 84 (1-2) ◽  
pp. 122-122
Author(s):  
Isabelle Kerblat ◽  
Catherine Aude ◽  
Christian Drouet ◽  
Heinet Niemann ◽  
Maurice Colomb
Keyword(s):  
T Cell ◽  
Light Chain ◽  
Tetanus Toxin ◽  
T Cell Clones ◽  
Human T Cell ◽  
Cell Clones

Myelin Basic Protein-Reactive Human T-Cell Clones: Stimulation by Diverse Microbial Antigens

1995 ◽  
Vol 756 (1 T-Cell Recept) ◽  
pp. 319-320 ◽  
Author(s):  
J. R. RICHERT ◽  
E. D. ROBINSON ◽  
A. H. JOHNSON ◽  
M. L. COHN ◽  
H. F. MCFARLAND ◽  
...  
Keyword(s):  
T Cell ◽  
Myelin Basic Protein ◽  
Basic Protein ◽  
T Cell Clones ◽  
Human T Cell ◽  
Cell Clones

Transfected murine cells expressing HLA class II can be used to generate alloreactive human T cell clones

1994 ◽  
Vol 169 (1) ◽  
pp. 25-33 ◽  
Author(s):  
Anthony N. Warrens ◽  
Tricia Heaton ◽  
Sid Sidhu ◽  
Giovanna Lombardi ◽  
Robert I. Lechler
Keyword(s):  
T Cell ◽  
Class Ii ◽  
Hla Class Ii ◽  
T Cell Clones ◽  
Human T Cell ◽  
Cell Clones
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