Cell-mediated Iympholysis: Examination of HLA genetic fine structure and complementation using cytotoxic lymphocytes

1978 ◽  
Vol 8 (9) ◽  
pp. 634-640 ◽  
Author(s):  
Dolores J. Schendel ◽  
R. Wank ◽  
B. Dupont
Keyword(s):  
Fine Structure ◽  
Cytotoxic Lymphocytes ◽  
Genetic Fine Structure

Mutations at the Saccharomyces cerevisiae SUP4 tRNA Tyr Locus: Isolation, Genetic Fine-Structure Mapping, and Correlation with Physical Structure

1982 ◽  
Vol 2 (12) ◽  
pp. 1501-1513
Author(s):  
Janet Kurjan ◽  
Benjamin D. Hall
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fine Structure ◽  
Physical Structure ◽  
Mapping Technique ◽  
Structure Mapping ◽  
Isolation And Characterization ◽  
Qualitative Estimate ◽  
Genetic Fine Structure ◽  
Entire Gene

The SUP4 tRNA Tyr locus in Saccharomyces cerevisiae has been studied by the isolation and characterization of mutations at the SUP4 gene which result in the loss of suppressor function. Most of the mutations act as single-site mutations, whereas about a third of the mutations are deletions of the entire gene. Two meiotic fine-structure maps of the gene were made. The first mapping technique placed 10 mutations plus the sup4 + anticodon on a map by a measurement of levels of recombination between pairs of mutations. The second map utilized a more qualitative estimate of recombination frequency, allowing 69 mutations and the sup4 + anticodon to be mapped. The maps were compared with the physical structure of the gene for the 34 mutations whose nucleotide alteration has been determined by DNA sequencing (Koski et al., Cell 22 :415-425, 1980; Kurjan et al., Cell 20 :701-709, 1980). Both maps show a good correlation with the physical structure of the gene, even though certain properties of genetic fine-structure maps, such as marker effects and “map expansion,” were seen.

scholarly journals Genetic Fine Structure of the Leucine Operon of Escherichia coli K-12

1973 ◽  
Vol 113 (3) ◽  
pp. 1268-1272 ◽  
Author(s):  
J. M. Somers ◽  
A. Amzallag ◽  
R. B. Middleton
Keyword(s):  
Escherichia Coli ◽  
Fine Structure ◽  
Genetic Fine Structure ◽  
K 12

scholarly journals Genetic fine-structure of the GA-1 locus in the higher plant Arabidopsis thaliana (L.) Heynh

1983 ◽  
Vol 41 (1) ◽  
pp. 57-68 ◽  
Author(s):  
M. Koornneef ◽  
J. Van Eden ◽  
C. J. Hanhart ◽  
A. M. M. De Jongh
Keyword(s):  
Arabidopsis Thaliana ◽  
Fine Structure ◽  
Higher Plants ◽  
Wild Type ◽  
Higher Plant ◽  
Selfed Progeny ◽  
Intragenic Deletion ◽  
Genetic Length ◽  
Genetic Fine Structure ◽  
Half Diallel

SUMMARYNon-germinating gibberellin (GA) responsive mutants are a powerful tool to study genetic fine structure in higher plants. Nine alleles (EMS-and fast neutron-induced) of the ga-1 locus of Arabidopsis thaliana were tested in a complete half-diallel. No wild type ‘recombinants’ were found in the selfed progeny of 9 homoallelic combinations (in total 3 × 105 plants); in the progenies from the 36 selfed hetero allelics the wild type frequency ranged from zero to 6·6 × 10−4. These frequencies allowed the construction of an internally consistent map for five different sites representing eight alleles. The ninth allele covered three sites and thus behaved like an intragenic deletion. The estimate of the total genetic length of the ga-1 locus was 0·07 cM. The order of the sites was also clearly reflected by the association with proximal outside markers. On the assumption that wild type gametes predominantly arise from reciprocal events, it was shown that a cross-over within the ga-1 locus leads to positive interference in the adjacent region.The results are discussed with respect to the mutagen used, the frequencies found in other plant and Drosophila genes, and the possible occurrence of gene conversion.

THE ROSY CISTRON IN DROSOPHILA MELANOGASTER: GENETIC FINE STRUCTURE ANALYSIS

Genetics ◽  
1964 ◽  
Vol 50 (6) ◽  
pp. 1245-1259 ◽  
Author(s):  
Arthur Chovnick ◽  
Abraham Schalet ◽  
R Peter Kernaghan ◽  
Marion Krauss
Keyword(s):  
Drosophila Melanogaster ◽  
Fine Structure ◽  
Structure Analysis ◽  
Fine Structure Analysis ◽  
Genetic Fine Structure

Distribution of Proflavin-Induced Mutations in the Genetic Fine Structure

Nature ◽  
1958 ◽  
Vol 182 (4641) ◽  
pp. 983-985 ◽  
Author(s):  
S. BRENNER ◽  
S. BENZER ◽  
L. BARNETT
Keyword(s):  
Fine Structure ◽  
Induced Mutations ◽  
Genetic Fine Structure

scholarly journals Deletion mapping of theniiAniaD gene region ofAspergillus nidulans

1979 ◽  
Vol 34 (1) ◽  
pp. 19-32 ◽  
Author(s):  
A. Brian Tomsett ◽  
David J. Cove
Keyword(s):  
Fine Structure ◽  
Aspergillus Nidulans ◽  
Gene Cluster ◽  
Nitrate Assimilation ◽  
Gene Region ◽  
Deletion Mapping ◽  
Mutagenic Treatment ◽  
Ofaspergillus Nidulans ◽  
Resistant Mutants ◽  
Genetic Fine Structure

SUMMARYThe genetic fine-structure of theniiAniaD gene region ofAspergillus nidulanshas been studied using deletion mapping. Deletions were identified asniiAniaD double mutants and comprised 1% of spontaneous chlorate-resistant mutants. All such double mutants were shown to involve deletions and their frequency was not increased by mutagenic treatment with eitherN-methyl-N′-nitro-N-nitrosoguanidine or with ultraviolet light. Deletion maps of theniaD andniiA genes have been constructed. A further class of mutation was also mapped using the deletions. Thesecrnmutations, which affect a gene whose function is as yet unknown, map on the centromere proximal side ofniiA. This analysis of a eukaryote gene cluster will provide a framework upon which to base studies of the regulation of the nitrate assimilation pathway.

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