scholarly journals Circulating markers of collagen types I, III, and IV in patients with dilated cardiomyopathy: relationships with myocardial collagen expression

2018 ◽  
Vol 5 (6) ◽  
pp. 1044-1051 ◽  
Author(s):  
Kazuya Nagao ◽  
Tsukasa Inada ◽  
Akinori Tamura ◽  
Kenji Kajitani ◽  
Kiyotaka Shimamura ◽  
...  
Keyword(s):  
Dilated Cardiomyopathy ◽  
Collagen Types ◽  
Collagen Expression ◽  
Circulating Markers ◽  
Myocardial Collagen

scholarly journals Tympanic Membrane Collagen Expression by Dynamically Cultured Human Mesenchymal Stromal Cell/Star-Branched Poly(ε-Caprolactone) Nonwoven Constructs

2020 ◽  
Vol 10 (9) ◽  
pp. 3043
Author(s):  
Stefania Moscato ◽  
Antonella Rocca ◽  
Delfo D’Alessandro ◽  
Dario Puppi ◽  
Vera Gramigna ◽  
...  
Keyword(s):  
Tympanic Membrane ◽  
Maximum Stress ◽  
Element Model ◽  
Von Mises Stress ◽  
Type I ◽  
Collagen Types ◽  
Collagen Expression ◽  
Polymerase Chain ◽  
Von Mises ◽  
Membrane Collagen

The tympanic membrane (TM) primes the sound transmission mechanism due to special fibrous layers mainly of collagens II, III, and IV as a product of TM fibroblasts, while type I is less represented. In this study, human mesenchymal stromal cells (hMSCs) were cultured on star-branched poly(ε-caprolactone) (*PCL)-based nonwovens using a TM bioreactor and proper differentiating factors to induce the expression of the TM collagen types. The cell cultures were carried out for one week under static and dynamic conditions. Reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC) were used to assess collagen expression. A Finite Element Model was applied to calculate the stress distribution on the scaffolds under dynamic culture. Nanohydroxyapatite (HA) was used as a filler to change density and tensile strength of *PCL scaffolds. In dynamically cultured *PCL constructs, fibroblast surface marker was overexpressed, and collagen type II was revealed via IHC. Collagen types I, III and IV were also detected. Von Mises stress maps showed that during the bioreactor motion, the maximum stress in *PCL was double that in HA/*PCL scaffolds. By using a *PCL nonwoven scaffold, with suitable physico-mechanical properties, an oscillatory culture, and proper differentiative factors, hMSCs were committed into fibroblast lineage-producing TM-like collagens.

scholarly journals Collagenases MMP-1, MMP-13, and Tissue Inhibitors TIMP-1, TIMP-2: Their Role in Healthy and Complicated Pregnancy and Potential as Preeclampsia Biomarkers—A Brief Review

2020 ◽  
Vol 10 (21) ◽  
pp. 7731
Author(s):  
Asparuh Nikolov ◽  
Nikola Popovski ◽  
Irena Hristova
Keyword(s):  
Matrix Metalloproteinases ◽  
Complex Structure ◽  
Fibrillar Collagen ◽  
Collagen Types ◽  
Tissue Inhibitors ◽  
Matrix Metalloproteinase 1 ◽  
The Matrix ◽  
Circulating Markers ◽  
Specific Tissue ◽  
Complicated Pregnancy

Extracellular matrix (ECM) turnover is characterized by a unique balance between matrix metalloproteinases’ degradation activity and their natural inhibition by collagen specific tissue inhibitors. Human uterine ECM is a complex structure, majorly consisting of proteins as fibrillar collagen types I and III, fibronectin, and laminin. Collagenases are enzymes from the matrix metalloproteinases’ family, which are predominantly involved in fibrillar collagen types I and III degradation. They are mainly represented by matrix metalloproteinase-1, -13 (MMP-1, -13), naturally inhibited by tissue inhibitors (TIMP-1, -2). The collagen structure of the uterus has been shown to be impaired in women with preeclampsia. This is a result of MMPs/TIMPs dysregulation interplay. This review article summarizes the actual available research data in the literature about the role of MMP-1, MMP-13 and TIMP-1, and TIMP-2 in collagen types I and III turnover in healthy and complicated pregnancy. Their potential use as circulating markers for diagnosis, prognosis, and monitoring of the development of preeclampsia is discussed as well.

scholarly journals Creatine kinase isoforms as circulating markers of deterioration in idiopathic dilated cardiomyopathy

1997 ◽  
Vol 20 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Mojgan Hossein-Nia ◽  
David W. Holt ◽  
William J. Mckenna ◽  
Kamran Baig ◽  
Jonathan H Goldman ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Dilated Cardiomyopathy ◽  
Idiopathic Dilated Cardiomyopathy ◽  
Circulating Markers

scholarly journals Increased gene expression of collagen Types I and III is inhibited by β-receptor blockade in patients with dilated cardiomyopathy

2005 ◽  
Vol 26 (24) ◽  
pp. 2698-2705 ◽  
Author(s):  
Junsho Shigeyama ◽  
Yoshio Yasumura ◽  
Aiji Sakamoto ◽  
Yoshio Ishida ◽  
Tatsuya Fukutomi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dilated Cardiomyopathy ◽  
Receptor Blockade ◽  
Collagen Types ◽  
Β Receptor

scholarly journals Creatine kinase isoforms as circulating markers of deterioration in idiopathic dilated cardiomyopathy

1996 ◽  
Vol 27 (2) ◽  
pp. 227
Author(s):  
Mojgan Hossein-Nia ◽  
Jonathan H. Goldman ◽  
Philip J. Keeling ◽  
William J. McKenna ◽  
David W. Holt
Keyword(s):  
Creatine Kinase ◽  
Dilated Cardiomyopathy ◽  
Idiopathic Dilated Cardiomyopathy ◽  
Circulating Markers
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