scholarly journals Analytical validation and field testing of a specific qPCR assay for environmental DNA detection of invasive European green crab ( Carcinus maenas )

2020 ◽  
Vol 2 (3) ◽  
pp. 309-320 ◽  
Author(s):  
Louise‐Marie D. Roux ◽  
Danièle Giblot‐Ducray ◽  
Nathan J. Bott ◽  
Kathryn H. Wiltshire ◽  
Marty R. Deveney ◽  
...  
Keyword(s):  
Dna Detection ◽  
Carcinus Maenas ◽  
Environmental Dna ◽  
Field Testing ◽  
Qpcr Assay ◽  
Green Crab ◽  
Analytical Validation ◽  
European Green Crab

scholarly journals Challenges in eDNA Detection of the Invasive European Green Crab, Carcinus Maenas

2021 ◽  
Author(s):  
Ariella M. Danziger ◽  
Markus Frederich
Keyword(s):  
Invasive Species ◽  
Visual Detection ◽  
Gulf Of Maine ◽  
Carcinus Maenas ◽  
Environmental Dna ◽  
Green Crab ◽  
Local Fauna ◽  
Irreversible Damage ◽  
European Green Crab ◽  
Species Specific

Abstract The early detection of invasive species is essential to cease the spread of the species before it can cause irreversible damage to the environment. The analysis of environmental DNA (eDNA) has emerged as a non-harmful method to detect the presence of a species before visual detection and is a promising approach to monitor invasive species. Few studies have investigated the use of eDNA for arthropods, as their exoskeleton is expected to limit the release of eDNA into the environment. We tested published primers for the invasive European green crab, Carcinus maenas, in the Gulf of Maine and found them not species-specific enough for reliable use outside of the area for which they were designed for. We then designed new primers, tested them against a broad range of local faunal species, and validated these primers in a field study. We demonstrate that eDNA analyses can be used for crustaceans with an exoskeleton and suggest that primers and probe sequences must be tested on local fauna at each location of use to ensure no positive amplification of these other species.

Linking eelgrass decline and impacts on associated fish communities to European green crab Carcinus maenas invasion

2016 ◽  
Vol 548 ◽  
pp. 31-45 ◽  
Author(s):  
K Matheson ◽  
CH McKenzie ◽  
RS Gregory ◽  
DA Robichaud ◽  
IR Bradbury ◽  
...  
Keyword(s):  
Carcinus Maenas ◽  
Fish Communities ◽  
Green Crab ◽  
European Green Crab
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