scholarly journals An urban Blitz with a twist: rapid biodiversity assessment using aquatic environmental DNA

2020 ◽  
Author(s):  
Kamil Hupało ◽  
Markus Majaneva ◽  
Molly Victoria Czachur ◽  
Lucas Sire ◽  
Daniel Marquina ◽  
...  
Keyword(s):  
Environmental Dna ◽  
Biodiversity Assessment ◽  
Rapid Biodiversity Assessment

scholarly journals Biodiversity soup: metabarcoding of arthropods for rapid biodiversity assessment and biomonitoring

2012 ◽  
Vol 3 (4) ◽  
pp. 613-623 ◽  
Author(s):  
Douglas W. Yu ◽  
Yinqiu Ji ◽  
Brent C. Emerson ◽  
Xiaoyang Wang ◽  
Chengxi Ye ◽  
...  
Keyword(s):  
Biodiversity Assessment ◽  
Rapid Biodiversity Assessment

Extracellular DNA in environmental samples: Occurrence, extraction, quantification, and impact on microbial biodiversity assessment

2021 ◽  
Author(s):  
Sakcham Bairoliya ◽  
Jonas Koh Zhi Xiang ◽  
Bin Cao
Keyword(s):  
Dna Sequencing ◽  
Microbial Communities ◽  
Dna Extraction ◽  
Environmental Samples ◽  
Environmental Dna ◽  
Extracellular Dna ◽  
Biodiversity Assessment ◽  
Microbial Biodiversity ◽  
Technical Details ◽  
The Impact

Environmental DNA, i.e., DNA directly extracted from environmental samples, has been applied to understand microbial communities in the environments and to monitor contemporary biodiversity in the conservation context. Environmental DNA often contains both intracellular DNA (iDNA) and extracellular DNA (eDNA). eDNA can persist in the environment and complicate environmental DNA sequencing-based analyses of microbial communities and biodiversity. Although several studies acknowledged the impact of eDNA on DNA-based profiling of environmental communities, eDNA is still being neglected or ignored in most studies dealing with environmental samples. In this article, we summarize key findings on eDNA in environmental samples and discuss the methods used to extract and quantify eDNA as well as the importance of eDNA on the interpretation of experimental results. We then suggest several factors to consider when designing experiments and analyzing data to negate or determine the contribution of eDNA to environmental DNA-based community analyses. This field of research will be driven forward by: (i) carefully designing environmental DNA extraction pipelines by taking into consideration technical details in methods for eDNA extraction/removal and membrane-based filtration and concentration; (ii) quantifying eDNA in extracted environmental DNA using multiple methods including qPCR and fluorescent DNA binding dyes; (iii) carefully interpretating effect of eDNA on DNA-based community analyses at different taxonomic levels; and (iv) when possible, removing eDNA from environmental samples for DNA-based community analyses.

scholarly journals Conservation and sustainable use of biodiversity in the islands and lagoons of northern Sri Lanka

2020 ◽  
Author(s):  
Devaka Weerakoon ◽  
Sampath De A. Goonatilake ◽  
Tharanga Wijewickrama ◽  
Arjan Rajasuriya ◽  
Naalin Perera ◽  
...  
Keyword(s):  
Civil War ◽  
Sri Lanka ◽  
Coastal Waters ◽  
Sustainable Use ◽  
Terrestrial Ecosystems ◽  
Northern Province ◽  
Biodiversity Assessment ◽  
Rapid Biodiversity Assessment ◽  
Palk Bay ◽  
The Government

After the 30-year long civil war was over, the Government of Sri Lanka commenced an accelerated programme to develop the Northern Province. If not carefully planned, such a programme will result in the loss of biodiversity and the consequent loss of services that ecosystems provide humans. Therefore, documenting the biodiversity found within coastal, nearshore and offshore islands of the Northern Province was identified as a need that would help both planners and conservation biologists alike. This report presents the results of a rapid biodiversity assessment of a 949 km stretch from Mannar to the Kokkilai Lagoon, including 22 coastal islands, five coastal stretches and four lagoons, extending across four districts (Jaffna, Mannar, Kilinochchi and Mullaittivu), 20 Divisional Secretariats Divisions and 264 Grama Niladhari Divisions. For terrestrial ecosystems, assessments were carried out during November 2015 and March 2016, and for marine surveys in the coastal waters of Palk Bay and Palk Strait, during November 2015 and March 2016.

A rapid biodiversity assessment methodology tested on intertidal rocky shores

2010 ◽  
Vol 20 (4) ◽  
pp. 452-463 ◽  
Author(s):  
Timothy D. O'hara ◽  
Prue F. E. Addison ◽  
Ruth Gazzard ◽  
Trudy L. Costa ◽  
Jacqueline B. Pocklington
Keyword(s):  
Rocky Shores ◽  
Biodiversity Assessment ◽  
Assessment Methodology ◽  
Rapid Biodiversity Assessment
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