scholarly journals Wolbachia pipientis occurs in Aedes aegypti populations in New Mexico and Florida, USA

2019 ◽  
Vol 9 (10) ◽  
pp. 6148-6156 ◽  
Author(s):  
Aditi Kulkarni ◽  
Wanqin Yu ◽  
Jinjin Jiang ◽  
Concepcion Sanchez ◽  
Ajit K. Karna ◽  
...  
Keyword(s):  
New Mexico ◽  
Aedes Aegypti ◽  
Wolbachia Pipientis

scholarly journals Wolbachia pipientis occurs in Aedes aegypti populations in New Mexico and Florida, USA

2018 ◽  
Author(s):  
Aditi Kulkarni ◽  
Wanqin Yu ◽  
Jinjin Jiang ◽  
Concepcion Sanchez ◽  
Ajit K. Karna ◽  
...  
Keyword(s):  
New Mexico ◽  
Aedes Aegypti ◽  
Control Strategies ◽  
Vector Competence ◽  
Virus Transmission ◽  
Lamp Assay ◽  
Harris County ◽  
Pcr Assay ◽  
Wolbachia Pipientis ◽  
Pcr Products

ABSTRACTThe mosquitoes Aedes aegypti (L.) and Ae. albopictus Skuse are the major vectors of dengue, Zika, yellow fever and chikungunya viruses worldwide. Wolbachia, an endosymbiotic bacterium present in many insects, is being utilized in novel vector control strategies to manipulate mosquito life history and vector competence to curb virus transmission. Earlier studies have found that Wolbachia is commonly detected in Ae. albopictus but rarely detected in Ae. aegypti. In this study, we used a two-step PCR assay to detect Wolbachia in wild-collected samples of Ae. aegypti. The PCR products were sequenced to validate amplicons and identify Wolbachia strains. A loop-mediated isothermal amplification (LAMP) assay was developed and used for detecting Wolbachia in selected mosquito specimens as well. We found Wolbachia in 85/148 (57.4%) wild Ae. aegypti specimens from various cities in New Mexico and in 2/46 (4.3%) from St. Augustine, Florida. We did not detect Wolbachia in 94 samples of Ae. aegypti from Deer Park, Harris County, Texas. Wolbachia detected in Ae. aegypti from both New Mexico and Florida was the wAlbB strain of Wolbachia pipientis. A Wolbachia positive colony of Ae. aegypti was established from pupae collected in Las Cruces, New Mexico in 2018. The infected females of this strain transmitted Wolbachia to their progeny when crossed with males of Rockefeller strain of Ae. aegypti, which does not carry Wolbachia. In contrast, none of the progeny of progeny of Las Cruces males mated to Rockefeller females were infected with Wolbachia.

scholarly journals Widespread insecticide resistance in Aedes aegypti L. from New Mexico, U.S.A.

PLoS ONE ◽  
2019 ◽  
Vol 14 (2) ◽  
pp. e0212693 ◽  
Author(s):  
Yashoda Kandel ◽  
Julia Vulcan ◽  
Stacy D. Rodriguez ◽  
Emily Moore ◽  
Hae-Na Chung ◽  
...  
Keyword(s):  
New Mexico ◽  
Aedes Aegypti ◽  
Insecticide Resistance

scholarly journals Increased locomotor activity and metabolism of Aedes aegypti infected with a life-shortening strain of Wolbachia pipientis

2009 ◽  
Vol 212 (10) ◽  
pp. 1436-1441 ◽  
Author(s):  
O. Evans ◽  
E. P. Caragata ◽  
C. J. McMeniman ◽  
M. Woolfit ◽  
D. C. Green ◽  
...  
Keyword(s):  
Locomotor Activity ◽  
Aedes Aegypti ◽  
Wolbachia Pipientis

scholarly journals How does competition among wild type mosquitoes influence the performance of Aedes aegypti and dissemination of Wolbachia pipientis?

2017 ◽  
Vol 11 (10) ◽  
pp. e0005947 ◽  
Author(s):  
Suellen de Oliveira ◽  
Daniel Antunes Maciel Villela ◽  
Fernando Braga Stehling Dias ◽  
Luciano Andrade Moreira ◽  
Rafael Maciel de Freitas
Keyword(s):  
Aedes Aegypti ◽  
Wild Type ◽  
Wolbachia Pipientis

scholarly journals Enfrentamento ao Aedes aegypti no contexto brasileiro

2019 ◽  
Vol 13 ◽  
Author(s):  
Diane Sales Vieira ◽  
Ítala Keane Rodrigues Dias ◽  
Cicera Luciana da Silva Sobreira ◽  
Francisco Elizaudo de Brito Junior ◽  
Maria Do Socorro Vieira Lopes
Keyword(s):  
Aedes Aegypti ◽  
Mosquito Control ◽  
Larval Fish ◽  
Hierarchical Classification ◽  
Pathogenic Fungi ◽  
Analysis Data ◽  
Botanical Insecticides ◽  
Wolbachia Pipientis ◽  
Innovative Techniques ◽  
Selection Of

Objetivo: sintetizar as ações desenvolvidas para o enfrentamento ao Aedes aegypti no contexto brasileiro. Método: trata-se de um estudo bibliográfico, descritivo, tipo revisão integrativa. Realizou-se a busca na MEDLINE, LILACS e BDENF. Utilizaram-se os filtros: texto completo disponível; idioma (português, inglês e espanhol), tipo de documento (artigo) e ano de publicação (2013-2018). Organizou-se o processo de definição e seleção dos estudos por meio do fluxograma PRISMA. Utilizou-se o software IRAMUTEQ para o processamento e a análise dos dados. Analisaram-se os dados a partir da Classificação Hierárquica Descendente e da nuvem de palavras. Resultados: selecionaram-se 31 estudos. Originaram-se seis classes com as seguintes estratégias: fungos entomopatogênicos; peixes larvívoros; Wolbachia pipientis; técnicas RILD e SIT; inseticidas botânicos; larvicidas Diflubenzuron e Deltramina; Piriproxifeno; monitoramento tecnológico; visitas regulares pelo ACS e ACE; abordagem ecossaúde; MIV e campanhas. Conclusão: conclui-se que há uma variedade de estratégias de enfrentamento às arboviroses e o quão são necessários o aperfeiçoamento e o desenvolvimento de técnicas inovadoras para o controle deste agravo. Descritores: Aedes; Controle de Mosquitos; Brasil; Prevenção de Doenças; Infecções por Arbovírus; Controle de Infecções.AbstractObjective: to synthesize the actions developed to confront Aedes aegypti in the Brazilian context. Method: this is a bibliographic, descriptive, integrative review study. The search was performed at MEDLINE, LILACS and BDENF. The filters were used: full text available; language (Portuguese, English and Spanish), type of document (article) and year of publication (2013-2018). The process of definition and selection of studies was organized through the PRISMA flowchart. IRAMUTEQ software was used for data processing and analysis. Data was analyzed from Descending Hierarchical Classification and word cloud. Results: 31 studies were selected. Six classes originated with the following strategies: entomo-pathogenic fungi; larval fish; Wolbachia pipientis; RILD and SIT techniques; botanical insecticides; larvicides Diflubenzuron and Deltramine; Pyriproxyfen; technological monitoring; regular visits by ACS and ACE; eco-health approach; MIV and campaigns. Conclusion: it is concluded that there are a variety of strategies for coping with arboviruses and how much the improvement and development of innovative techniques for the control of this disease are necessary. Descriptors: Aedes; Mosquito Control; Brazil; Disease Prevention; Arbovirus Infections; Infection Control.ResumenObjetivo: sintetizar las acciones desarrolladas para enfrentar al Aedes aegypti en el contexto brasileño. Método: este es un estudio bibliográfico, descriptivo, tipo revisión integradora. La búsqueda se realizó en MEDLINE, LILACS y BDENF. Se utilizaron los filtros: texto completo disponible; idioma (portugués, inglés y español), tipo de documento (artículo) y año de publicación (2013-2018). El proceso de definición y selección de estudios se organizó a través del diagrama de flujo PRISMA. El software IRAMUTEQ se utilizó para el procesamiento y análisis de datos. Los datos se analizaron a partir de Clasificación Jerárquica Descendente y nube de palabras. Resultados: se seleccionaron 31 estudios. Seis clases se originaron con las siguientes estrategias: hongos entomopatógenos; peces larvales; Wolbachia pipientis; Técnicas RILD y SIT; insecticidas botánicos; larvicidas Diflubenzuron y Deltramine; Piriproxifeno; monitoreo tecnológico; visitas regulares de ACS y ACE; enfoque de ecosalud; MIV y campañas. Conclusión: se concluye que hay una variedad de estrategias para hacer frente a los arbovirus y cuánto es necesaria la mejora y el desarrollo de técnicas innovadoras para el control de esta enfermedad. Descriptores: Aedes; Control de Mosquitos; Brasil; Prevención de Enfermedades; Infecciones por Arbovirus; Control de Infecciones.

scholarly journals Comprehensive quantitative proteome analysis of Aedes aegypti identifies proteins and pathways involved in Wolbachia pipientis and Zika virus interference phenomenon

2020 ◽  
Author(s):  
Michele Martins ◽  
Luis Felipe Costa Ramos ◽  
Jimmy Rodriguez Murillo ◽  
André Torres ◽  
Stephanie Serafim de Carvalho ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Immune Response ◽  
Aedes Aegypti ◽  
Humoral Immune Response ◽  
Zika Virus ◽  
Mosquito Vector ◽  
Ros Production ◽  
Zikv Infection ◽  
Wolbachia Pipientis ◽  
Humoral Immune

ABSTRACTZika virus is a global public health emergency due to its association with microcephaly, Guillain-Barré syndrome, neuropathy, and myelitis in children and adults. A total of 87 countries have had evidence of autochthonous mosquito-borne transmission of Zika virus, distributed across four continents, and no antivirus therapy or vaccines are available. Therefore, several strategies have been developed to target the main mosquito vector, Aedes aegypti, to reduce the burden of different arboviruses. Among such strategies, the use of the maternally-inherited endosymbiont Wolbachia pipientis has been applied successfully to reduce virus susceptibility and decrease transmission. However, the mechanisms by which Wolbachia orchestrate resistance to ZIKV infection remain to be elucidated. In this study, we apply isobaric labeling quantitative mass spectrometry-based proteomics to quantify proteins and identify pathways altered during ZIKV infection; Wolbachia infection; co-infection with Wolbachia/ZIKV in the Ae. aegypti heads and salivary glands. We show that Wolbachia regulates proteins involved in ROS production, regulates humoral immune response, and antioxidant production. The reduction of ZIKV polyprotein in the presence of Wolbachia in mosquitoes was determined by mass spectrometry and corroborates the idea that Wolbachia helps to block ZIKV infections in Ae. aegypti. The present study offers a rich resource of data that may help to elucidate mechanisms by which Wolbachia orchestrate resistance to ZIKV infection in Ae. aegypti, and represents a step further on the development of new targeted methods to detect and quantify ZIKV and Wolbachia directly in complex tissues.HighlightsThe abundance of ZIKV polyprotein is reduced in the presence of WolbachiaShotgun proteomics quantifies ZIKV and Wolbachia proteins directly in tissuesWolbachia regulates proteins involved in ROS productionWolbachia regulates humoral immune response and antioxidant productionMetabolism and detoxification processes were associated with mono infections

scholarly journals Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon

2021 ◽  
Vol 12 ◽  
Author(s):  
Michele Martins ◽  
Luis Felipe Costa Ramos ◽  
Jimmy Rodriguez Murillo ◽  
André Torres ◽  
Stephanie Serafim de Carvalho ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Zika Virus ◽  
Proteome Analysis ◽  
Mosquito Vector ◽  
Global Public Health ◽  
Zikv Infection ◽  
Interference Phenomenon ◽  
Wolbachia Pipientis ◽  
Humoral Immune ◽  
Species Production

Zika virus (ZIKV) is a global public health emergency due to its association with microcephaly, Guillain-Barré syndrome, neuropathy, and myelitis in children and adults. A total of 87 countries have had evidence of autochthonous mosquito-borne transmission of ZIKV, distributed across four continents, and no antivirus therapy or vaccines are available. Therefore, several strategies have been developed to target the main mosquito vector, Aedes aegypti, to reduce the burden of different arboviruses. Among such strategies, the use of the maternally-inherited endosymbiont Wolbachia pipientis has been applied successfully to reduce virus susceptibility and decrease transmission. However, the mechanisms by which Wolbachia orchestrate resistance to ZIKV infection remain to be elucidated. In this study, we apply isobaric labeling quantitative mass spectrometry (MS)-based proteomics to quantify proteins and identify pathways altered during ZIKV infection; Wolbachia infection; co-infection with Wolbachia/ZIKV in the A. aegypti heads and salivary glands. We show that Wolbachia regulates proteins involved in reactive oxygen species production, regulates humoral immune response, and antioxidant production. The reduction of ZIKV polyprotein in the presence of Wolbachia in mosquitoes was determined by MS and corroborates the idea that Wolbachia helps to block ZIKV infections in A. aegypti. The present study offers a rich resource of data that may help to elucidate mechanisms by which Wolbachia orchestrate resistance to ZIKV infection in A. aegypti, and represents a step further on the development of new targeted methods to detect and quantify ZIKV and Wolbachia directly in complex tissues.

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