scholarly journals Repurposing population genetics data to discern genomic architecture: A case study of linkage cohort detection in mountain pine beetle ( Dendroctonus ponderosae )

2018 ◽  
Vol 9 (3) ◽  
pp. 1147-1159 ◽  
Author(s):  
Stephen A. L. Trevoy ◽  
Jasmine K. Janes ◽  
Kevin Muirhead ◽  
Felix A. H. Sperling
Keyword(s):  
Population Genetics ◽  
Mountain Pine Beetle ◽  
Dendroctonus Ponderosae ◽  
Genomic Architecture ◽  
Mountain Pine ◽  
Pine Beetle

scholarly journals Chromosome-level genome assembly reveals genomic architecture of northern range expansion in the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae)

2021 ◽  
Author(s):  
Christopher Keeling ◽  
Erin Campbell ◽  
Philip Batista ◽  
Victor Shegelski ◽  
Stephen Trevoy ◽  
...  
Keyword(s):  
X Chromosome ◽  
Linkage Mapping ◽  
Mountain Pine Beetle ◽  
Population Genomics ◽  
Dendroctonus Ponderosae ◽  
Draft Genome ◽  
Bioinformatic Tools ◽  
Genomic Architecture ◽  
Mountain Pine ◽  
Pine Beetle

Genome sequencing methods and assembly tools have improved dramatically since the 2013 publication of draft genome assemblies for the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae). We conducted proximity ligation library sequencing and scaffolding to improve contiguity, and then used linkage mapping and recent bioinformatic tools for correction and further improvement. The new assemblies have dramatically improved contiguity and gaps compared to the originals: N50 values increased 26- to 36-fold, and the number of gaps were reduced by half. Ninety percent of the content of the assemblies is now contained in 12 and 11 scaffolds for the female and male assemblies, respectively. Based on linkage mapping information, the 12 largest scaffolds in both assemblies represent all 11 autosomal chromosomes and the neo-X chromosome. These assemblies now have nearly chromosome-sized scaffolds and will be instrumental for studying genomic architecture, chromosome evolution, population genomics, functional genomics, and adaptation in this and other pest insects. We also identified regions in two chromosomes, including the ancestral-X portion of the neo-X chromosome, with elevated differentiation between northern and southern Canadian populations.

scholarly journals Chromosome-level genome assembly reveals genomic architecture of northern range expansion in the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae)

2021 ◽  
Author(s):  
Christopher I. Keeling ◽  
Erin O. Campbell ◽  
Philip D. Batista ◽  
Victor A. Shegelski ◽  
Stephen A. L. Trevoy ◽  
...  
Keyword(s):  
X Chromosome ◽  
Linkage Mapping ◽  
Mountain Pine Beetle ◽  
Population Genomics ◽  
Dendroctonus Ponderosae ◽  
Draft Genome ◽  
Bioinformatic Tools ◽  
Genomic Architecture ◽  
Mountain Pine ◽  
Pine Beetle

Genome sequencing methods and assembly tools have improved dramatically since the 2013 publication of draft genome assemblies for the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae). We conducted proximity ligation library sequencing and scaffolding to improve contiguity, and then used linkage mapping and recent bioinformatic tools for correction and further improvement. The new assemblies have dramatically improved contiguity and gaps compared to the originals: N50 values increased 26- to 36-fold, and the number of gaps were reduced by half. Ninety percent of the content of the assemblies is now contained in 12 and 11 scaffolds for the female and male assemblies, respectively. Based on linkage mapping information, the 12 largest scaffolds in both assemblies represent all 11 autosomal chromosomes and the neo-X chromosome. These assemblies now have nearly chromosome-sized scaffolds and will be instrumental for studying genomic architecture, chromosome evolution, population genomics, functional genomics, and adaptation in this and other pest insects. We also identified regions in two chromosomes, including the ancestral-X portion of the neo-X chromosome, with elevated differentiation between northern and southern Canadian populations.

Spatial genetic structure of the mountain pine beetle (Dendroctonus ponderosae) outbreak in western Canada: historical patterns and contemporary dispersal

2012 ◽  
Vol 21 (12) ◽  
pp. 2931-2948 ◽  
Author(s):  
G. D. N. GAYATHRI SAMARASEKERA ◽  
NICHOLAS V. BARTELL ◽  
B. STAFFAN LINDGREN ◽  
JANICE E. K. COOKE ◽  
COREY S. DAVIS ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Mountain Pine Beetle ◽  
Spatial Genetic Structure ◽  
Dendroctonus Ponderosae ◽  
Western Canada ◽  
Mountain Pine ◽  
Pine Beetle

OBSERVATIONS ON A TRIAL OF BROADCAST BURNING TO CONTROL AN INFESTATION OF THE MOUNTAIN PINE BEETLE DENDROCTONUS PONDEROSAE

1989 ◽  
Vol 121 (6) ◽  
pp. 521-523 ◽  
Author(s):  
A.J. Stock ◽  
R.A. Gorley
Keyword(s):  
Pinus Contorta ◽  
Mountain Pine Beetle ◽  
Lodgepole Pine ◽  
Dendroctonus Ponderosae ◽  
Management Plan ◽  
Management Program ◽  
Control Measures ◽  
Direct Control ◽  
Mountain Pine ◽  
Pine Beetle

The mountain pine beetle, Dendroctonus ponderosae Hopk., causes extensive mortality of lodgepole pine, Pinus contorta var. latifolia Engelm., throughout western North America (Van Sickle 1982). The Prince Rupert Forest Region, in the northwest of British Columbia, initiated an aggressive beetle management program in 1981. Logging of infested stands, and winter felling and burning of individual infested trees are the most common direct control techniques.The “Bristol Lake” infestation developed in the Bulkley Forest District, approximately 55 km northwest of Smithers, B.C., on a steep rocky ridge within the valley of Harold Price Creek. The area contained large volumes of mature lodgepole pine, and control of the infestation was therefore considered critical to the local beetle management plan, but the size (50 ha) and rough topography of the infested area precluded normal direct control measures.

Evaluation of the push-pull tactic against the mountain pine beetle using verbenone and non-host volatiles in combination with pheromone-baited trees

2006 ◽  
Vol 82 (4) ◽  
pp. 579-590 ◽  
Author(s):  
John H Borden ◽  
Anna L Birmingham ◽  
Jennifer S Burleigh
Keyword(s):  
Pest Management ◽  
Untreated Control ◽  
Pinus Contorta ◽  
Mountain Pine Beetle ◽  
Dendroctonus Ponderosae ◽  
Wide Band ◽  
Mountain Pine ◽  
Pine Beetle ◽  
The Mean ◽  
Lodgepole Pines

Experiments were conducted near Williams Lake and Quesnel, BC in 2003 to evaluate the effectiveness of the anti-aggregation pheromone verbenone and a three-component non-host volatile (NHV) blend (E-2- and Z-3-hexen-1-ol and benzyl alcohol) in deterring attack of lodgepole pines, Pinus contorta var. latifolia Engelmann, by the mountain pine beetle, Dendroctonus ponderosae Hopkins. In 0.16-ha square plots, with a pheromone-baited tree in the centre and 16 release points at 10-m centres, either verbenone (in a polyurethane gel inside plastic membrane pouches, released at ca. 100 mg/day) or the NHVs (released from separate bubble caps at ca. 1.2 mg/day) deterred attack, but efficacy was not increased by combining them. When deployed from 25 release points at 10-m centres in 0.25-ha square plots, verbenone plus NHVs were effective in deterring attack in some (but not all) cases, when compared to attack in a 25-m wide band around the treated zone. In a test of the push-pull tactic, verbenone plus the NHV blend were tested in a 10-replicate experiment with 100, 44.4 or 25 release points/ha at 10-, 15- or 20-m centres, respectively, in a 1-ha square central zone surrounded by a 3-ha, 50-m-wide band containing 12 pheromone-baited lodgepole pines 50 m apart. Other treatments were pheromonebaited trees alone, and an untreated control. In the three push-pull treatments (but not the bait only or control treatments), 28 of 30 replicates had significantly more mass-attacked trees in the pheromone-baited outer 3 ha than in the inner ha treated with verbenone plus NHVs. The percentage of available trees ≥ 17.5 cm diameter at breast height (dbh) that were mass-attacked was < 10% in 5, 4 and 3 of 10 replicates when verbenone plus NHVs were deployed at 10-, 15- and 20-m centres, respectively, and was < 10% in two each of the bait only and control replicates. The mean ratios of newly-attacked green trees in 2003 to red trees killed in 2002 were significantly lower in the inner ha of the 10-m and 15-m centre treatments (2.6 and 2.7, respectively) than 5.9 in the untreated control. Also the pooled percentages of attacked trees that were not mass-attacked were significantly higher in the inner ha of the treatments with centres at 15 m (24.7%) and 10 m (17.6%) than in the other three treatments (all between 12% and 13%). Despite the apparent efficacy in 10-m and 15-m centre treatments, some replicates failed spectacularly. Failure was not significantly related to the incidence of red trees, but was negatively related to density/ha of available trees and positively related to mean dbh. We recommend operational implementation of the push-pull tactic at 10-m or 15-m centres when the density of available lodgepole pines is > 400/ha, the mean dbh is ≤ 25 cm, current attack is ≤ 15%, and the tactic is part of an integrated pest management program that includes sanitation harvesting. Using verbenone alone at 15-m centres would cost $380/ha (CAD), excluding labour. Key words: mountain pine beetle, Dendroctonus ponderosae, lodgepole pine, Pinus contorta var. latifolia, pheromones, semiochemicals, pest management

Acoustics of the mountain pine beetle (Dendroctonus ponderosae) (Curculionidae, Scolytinae): sonic, ultrasonic, and vibration characteristics

2013 ◽  
Vol 91 (4) ◽  
pp. 235-244 ◽  
Author(s):  
A.J. Fleming ◽  
A.A. Lindeman ◽  
A.L. Carroll ◽  
J.E. Yack
Keyword(s):  
Bark Beetles ◽  
Mountain Pine Beetle ◽  
Dendroctonus Ponderosae ◽  
Laser Doppler Vibrometer ◽  
Future Research ◽  
Acoustic Signaling ◽  
Mountain Pine ◽  
Pine Beetle ◽  
Close Range ◽  
Low Amplitude

Acoustic signaling is widespread in bark beetles (Scolytinae), although little is known about the physical characteristics of signals, how they are transmitted, and how they differ among behavioural contexts. Signals were studied in the male mountain pine beetle (Dendroctonus ponderosae Hopkins, 1902) during stress, male–female, and male–male interactions. Sounds are broadband with significant energy in the ultrasound (peaks between 15 and 26 kHz) and low amplitude (55 and 47 dB SPL at 2 and 4 cm, respectively), indicating that signaling functions at close range. Signal trains vary among contexts primarily in the proportions of chirp types. Chirps were categorized as being simple or interrupted, with the former having significantly lower tooth strike rates and shorter chirp durations. Stress chirps are predominantly simple with characteristics resembling other insect disturbance signals. Male–female interactions begin with the male producing predominantly interrupted chirps prior to gallery entrance, followed by simple chirps. Male–male (rivalry) chirps are predominantly simple, with evidence of antiphonal calling. Substrate-borne vibrations were detectable with a laser-doppler vibrometer at short distances (1–3 cm), suggesting that sensory organs could be tuned to either air or substrate-borne vibrations. These results have important implications for future research on the function and reception of acoustic signals in bark beetles.

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