Combined effects of local habitat, anthropogenic stress, and dispersal on stream ecosystems: a mesocosm experiment

Jarno Turunen; Pauliina Louhi; Heikki Mykrä; Jukka Aroviita; Emmi Putkonen; Ari Huusko; Timo Muotka

doi:10.1002/eap.1762

Multiple stress – combined effects of natural and anthropogenic stress factors on organisms in boreal lakes

SIL Proceedings 1922-2010 ◽

10.1080/03680770.2001.11901858 ◽

2002 ◽

Vol 28 (2) ◽

pp. 958-961

Author(s):

Ismo J. Holopainen ◽

Jari Heinonen ◽

Olli-Pekka Penttinen ◽

Jussi V. K. Kukkonen

Keyword(s):

Boreal Lakes ◽

Stress Factors ◽

Combined Effects ◽

Anthropogenic Stress ◽

Multiple Stress

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Individual and combined effects of fine sediment and glyphosate herbicide on invertebrate drift and insect emergence: a stream mesocosm experiment

Freshwater Science ◽

10.1086/684363 ◽

2016 ◽

Vol 35 (1) ◽

pp. 139-151 ◽

Cited By ~ 14

Author(s):

Francis S. Magbanua ◽

Colin R. Townsend ◽

Kimberly J. Hageman ◽

Jeremy J. Piggott ◽

Christoph D. Matthaei

Keyword(s):

Fine Sediment ◽

Mesocosm Experiment ◽

Invertebrate Drift ◽

Combined Effects ◽

Insect Emergence ◽

Stream Mesocosm ◽

Glyphosate Herbicide

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Forecasting the combined effects of urbanization and climate change on stream ecosystems: from impacts to management options

Journal of Applied Ecology ◽

10.1111/j.1365-2664.2008.01599.x ◽

2009 ◽

Vol 46 (1) ◽

pp. 154-163 ◽

Cited By ~ 96

Author(s):

Kären C. Nelson ◽

Margaret A. Palmer ◽

James E. Pizzuto ◽

Glenn E. Moglen ◽

Paul L. Angermeier ◽

...

Keyword(s):

Climate Change ◽

Stream Ecosystems ◽

Combined Effects ◽

Management Options

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Metals alter membership but not diversity of a headwater stream microbiome

10.1101/2020.04.30.071522 ◽

2020 ◽

Author(s):

Brian A. Wolff ◽

William H. Clements ◽

Ed K. Hall

Keyword(s):

Metal Contamination ◽

Illumina Miseq ◽

Metal Stress ◽

Mesocosm Experiment ◽

Metal Exposure ◽

Stream Ecosystems ◽

Rrna Gene ◽

Surface Water Chemistry ◽

Routine Sampling ◽

Study Sites

AbstractMetal contamination from mining or natural weathering is a common feature of surface waters in the American west. Traditionally, stream macroinvertebrate community metrics have been used for stream quality assessments. Advances in microbial analyses have created the potential for routine sampling of aquatic microbiomes as a tool to assess the quality of stream habitat. We sought to determine if microbiome diversity and membership were affected by metal contamination in a manner similar to what has been observed for stream macroinvertebrates, and if so, identify candidate microbial taxa to be used to indicate metal stress in stream ecosystems. We evaluated microbiome membership from sediments at multiple sites within the principal drainage of an EPA superfund site near the headwaters of the Upper Arkansas River, Leadville, CO. From each sample, we extracted DNA and sequenced the 16S rRNA gene amplicon on the Illumina MiSeq platform. We used the remaining sediments to simultaneously evaluate environmental metal concentrations. We also conducted an artificial stream mesocosm experiment using sediments collected from two of the observational study sites. The mesocosm experiment had a 2×2 factorial design: 1) location (upstream or downstream of contaminating tributary), and 2) treatment (metal exposure or control). We found no difference in diversity between upstream and downstream sites in the field. Similarly, diversity changed very little following experimental metal exposure. However, microbiome membership differed between upstream and downstream locations and experimental metal exposure changed microbiome membership in a manner that depended on origin of the sediments used in each mesocosm.ImportanceOur results suggest that microbiomes can be reliable indicators of ecosystem metal stress even when surface water chemistry and other metrics used to assess ecosystem health do not indicate ecosystem stress. Several results presented in this study are consistent with the idea that a microbial response to metals at the base of the food web may be affecting consumers one trophic level above. If effects of metals are mediated through shifts in the microbiome, then microbial metrics, as presented here, may aid in the assessment of stream ecosystems health.

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Combined effects of fish and macroinvertebrate predation on zooplankton in a littoral mesocosm experiment

Hydrobiologia ◽

10.1007/s10750-018-3712-y ◽

2018 ◽

Vol 829 (1) ◽

pp. 19-29 ◽

Cited By ~ 6

Author(s):

A. Mamani ◽

M. L. Koncurat ◽

M. Boveri

Keyword(s):

Mesocosm Experiment ◽

Combined Effects

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Responses of a eutrophic pond community to separate and combined effects of N:P supply and planktivorous fish: a mesocosm experiment

Hydrobiologia ◽

10.1007/bf00007782 ◽

1990 ◽

Vol 208 (3) ◽

pp. 161-167 ◽

Cited By ~ 20

Author(s):

Ray W. Drenner ◽

J. Durward Smith ◽

John R. Mummert ◽

Holli F. Lancaster

Keyword(s):

Mesocosm Experiment ◽

Planktivorous Fish ◽

Combined Effects

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Effects of Retinoic Acid and 1,25-Dihydroxyvitamin D3 on IFN-gamma Secretion by Mononuclear Leukocytes from Nulliparous and Postparturient Dairy Cattle

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831.70.3.92 ◽

2000 ◽

Vol 70 (3) ◽

pp. 92-101 ◽

Cited By ~ 14

Author(s):

Burim Ametaj ◽

Brian Nonnecke ◽

Ronald Horst ◽

Donald Beitz

Keyword(s):

Retinoic Acid ◽

Dairy Cattle ◽

Dairy Cows ◽

Dairy Cow ◽

Pokeweed Mitogen ◽

Mononuclear Leukocytes ◽

Combined Effects ◽

Ifn Gamma ◽

Dihydroxyvitamin D ◽

1,25 Dihydroxyvitamin D

Individual and combined effects of several isomers of retinoic acid (RA) and 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on interferon-gamma (IFN-gamma) secretion by blood mononuclear leukocytes (MNL) from nulliparous and postparturient Holstein cattle were evaluated in vitro. In the first experiment, effects on incubation period (24 to 72 hours) and time of supplementation (0 to 32 hours) with all-trans, 9-cis, 13-cis-, and 9,13-dicis-RAs (0 to 100 nM) on IFN-gamma secretion by pokeweed mitogen (PWM)-stimulated (0 and 10 mug/ml) MNL from nulliparous cattle were evaluated. In the second experiment, MNL from postparturient cows (bled at 0, 2, 4, and 16 days postpartum) were stimulated with PWM (0 and 10 mug/ml) in the presence of RA isomers (9-cis- or 9,13-dicis-RA; 0 to 100 nM), 1,25-(OH)2D3 (0 to 100 nM), or with combinations of these metabolites. The results show that individual isomers of RA had no effect on IFN-gamma secretion by PWM-stimulated MNL from nulliparous or postparturient cows. Furthermore 1,25-dihydroxyvitamin D3 inhibited IFN-gamma secretion by MNL from nulliparous and postparturient dairy cows; however, the degree of inhibition was greater when 9-cis- and 9,13-dicis-RA were also present in the cultures. Finally mononuclear leukocytes from postparturient dairy cows produced substantially less IFN-gamma than did MNL from nulliparous cattle. It is concluded that retinoic acids individually did not affect the capacity of leukocytes from dairy cattle to secrete IFN-gamma. This result is in marked contrast to studies in monogastric species indicating that RAs inhibit IFN-gamma secretion by peripheral blood T cells. Inhibition of IFN-gamma secretion by 1,25-(OH)2D3 was potentiated by 9-cis- and 9,13-di-cis-retinoics acids, suggesting that an excess of dietary vitamins A and D may compromise further the naturally immunosuppressed postparturient dairy cow. Additional research is necessary to determine if the combined effects of these metabolites on IFN-gamma secretion represent an increased susceptibility of the dairy cow to infectious diseases during the periparturient period. Lower secretion of IFN-gamma by MNL from postpartutient dairy cows, relative to nulliparous cattle, suggests that recently-calved cows are naturally immunosuppressed.

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