Sox9 CreER ‐mediated deletion of β‐catenin in palatal mesenchyme results in delayed palatal elevation accompanied with repressed canonical Wnt signaling and reduced actin polymerization

genesis ◽  
2021 ◽  
Author(s):  
Xiaoxiao Pang ◽  
Xiaoming Wang ◽  
Yahong Wang ◽  
Lingling Pu ◽  
Jiayu Shi ◽  
...  
Keyword(s):  
Wnt Signaling ◽  
Actin Polymerization ◽  
Canonical Wnt Signaling ◽  
Canonical Wnt

scholarly journals Daam2 couples translocation and clustering of Wnt receptor signalosomes through Rac1

2020 ◽  
pp. jcs.251140
Author(s):  
Carlo D. Cristobal ◽  
Qi Ye ◽  
Juyeon Jo ◽  
Xiaoyun Ding ◽  
Chih-Yen Wang ◽  
...  
Keyword(s):  
Wnt Signaling ◽  
Actin Polymerization ◽  
Critical Role ◽  
Dependent Manner ◽  
Canonical Wnt Signaling ◽  
Downstream Signaling ◽  
Receptor Complexes ◽  
Spinal Cord Dorsal ◽  
Canonical Wnt ◽  
Chick Spinal Cord

Wnt signaling plays a critical role in development across species and is dysregulated in a host of human diseases. A key step in signal transduction is the formation of Wnt receptor signalosomes, where a large number of components translocate to the membrane, cluster together, and amplify downstream signaling. However, the molecular processes that coordinate these events remain poorly defined. Here, we show that Daam2 regulates canonical Wnt signaling via the PIP2-PIP5K axis through its association with Rac1. Clustering of Daam2-mediated Wnt receptor complexes requires both Rac1 and PIP5K, and PIP5K promotes membrane localization of these complexes in a Rac1-dependent manner. Importantly, the localization of Daam2 complexes and Daam2-mediated canonical Wnt signaling is dependent upon actin polymerization. These studies highlight novel roles for Rac1 and the actin cytoskeleton in the regulation of canonical Wnt signaling and define Daam2 as a key scaffolding hub that coordinates membrane translocation and signalosome clustering. Key words: Daam2, Rac1, PIP5K, Wnt signalosome, chick spinal cord, dorsal patterning

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