Axin2-mTurquoise2: A novel reporter mouse model for the detection of canonical Wnt signalling

genesis ◽  
2017 ◽  
Vol 55 (10) ◽  
pp. e23068 ◽  
Author(s):  
Jolanda. J. D. de Roo ◽  
Cor Breukel ◽  
Amiet R. Chhatta ◽  
Margot M. Linssen ◽  
Sandra A. Vloemans ◽  
...  
Keyword(s):  
Mouse Model ◽  
Wnt Signalling ◽  
Reporter Mouse ◽  
Canonical Wnt

scholarly journals TMOD-24. GENERATION AND CHARACTERIZATION OF A NOVEL TRANSGENIC IDO REPORTER MOUSE FOR IDO POSTTRANSLATIONAL MODIFICATION ANALYSIS IN SITU

2020 ◽  
Vol 22 (Supplement_2) ◽  
pp. ii233-ii233
Author(s):  
April Bell ◽  
Lijie Zhai ◽  
Erik Ladomersky ◽  
Kristen Lauing ◽  
Lakshmi Bollu ◽  
...  
Keyword(s):  
Mouse Model ◽  
Tumor Cell ◽  
Central Nervous System Tumor ◽  
Post Translational Modifications ◽  
Reporter Mouse ◽  
C Terminus ◽  
Human Gbm

Abstract Glioblastoma (GBM) is the most common and aggressive primary central nervous system tumor in adults with a median survival of 14.6 months. GBM is a potently immunosuppressive cancer due in-part to the prolific expression of immunosuppressive indoleamine 2,3 dioxygenase 1 (IDO). Tumor cell IDO facilitates the intratumoral accumulation of regulatory T cells (Tregs; CD4+CD25+FoxP3+). Although immunosuppressive IDO activity is canonically characterized by the conversion of tryptophan into kynurenine, we have utilized transgenic and syngeneic mouse models and mutant glioma lines to demonstrate that tumor cell IDO increases Treg accumulation independent of tryptophan metabolism. Here, we address the gap in our understanding of IDO signaling activity in vivo. Subcutaneously-engrafted human GBM expressing human IDO-GFP cDNA was isolated from immunodeficient humanized NSG-SGM3 mice. The tumor was immunoprecipitated for the GFP tag using GFP-TRAP followed by mass spectrometry which revealed a novel methylation site on a lysine residue at amino acid 373 in the IDO C-terminus region. Western blot analysis of IDO protein also revealed the presence of tyrosine phosphorylation. Additionally, we recently created a new transgenic IDO reporter mouse model whereby endogenous IDO is fused to GFP via a T2A linker (IDO→GFP). This model allows for the isolation of IDO+ cells in real-time and without causing cell death, thereby creating the opportunity for downstream molecular analysis of in situ-isolated GFP+ cells. Collectively, our work suggests that IDO non-enzyme activity may involve the post-translational modifications we recently identified. As IDO activity may differ between in vitro and in vivo modeling systems, we will use the new IDO→GFP reporter mouse model for an improved mechanistic understanding of how immunosuppressive IDO facilitates Treg accumulation in vivo.

scholarly journals Corrigendum to “Cyclin G2 Suppresses Glomerulosclerosis by Regulating Canonical Wnt Signalling”

2019 ◽  
Vol 2019 ◽  
pp. 1-1
Author(s):  
Chenyang Zhao ◽  
Jinlan Gao ◽  
Sen Li ◽  
Qi Liu ◽  
Xiaoyu Hou ◽  
...  
Keyword(s):  
Wnt Signalling ◽  
Cyclin G2 ◽  
Canonical Wnt

scholarly journals A dual Ucp1 reporter mouse model for imaging and quantitation of brown and brite fat recruitment

2019 ◽  
Vol 20 ◽  
pp. 14-27 ◽  
Author(s):  
Hui Wang ◽  
Monja Willershäuser ◽  
Angelos Karlas ◽  
Dimitris Gorpas ◽  
Josefine Reber ◽  
...  
Keyword(s):  
Mouse Model ◽  
Reporter Mouse

scholarly journals PTK7/Otk interacts with Wnts and inhibits canonical Wnt signalling

2011 ◽  
Vol 30 (18) ◽  
pp. 3729-3740 ◽  
Author(s):  
Hanna Peradziryi ◽  
Nicole A Kaplan ◽  
Martina Podleschny ◽  
Xiaoping Liu ◽  
Peter Wehner ◽  
...  
Keyword(s):  
Wnt Signalling ◽  
Canonical Wnt
Sign in / Sign up

Export Citation Format

Share Document