scholarly journals Nkx2.2:Cre knock-in mouse line: A novel tool for pancreas- and CNS-specific gene deletion

genesis ◽  
2013 ◽  
Vol 51 (12) ◽  
pp. 844-851 ◽  
Author(s):  
Dina A. Balderes ◽  
Mark A. Magnuson ◽  
Lori Sussel
Keyword(s):  
Gene Deletion ◽  
Specific Gene ◽  
Mouse Line

scholarly journals Gfi1-Cre knock-in mouse line: A tool for inner ear hair cell-specific gene deletion

genesis ◽  
2010 ◽  
Vol 48 (6) ◽  
pp. 400-406 ◽  
Author(s):  
Hua Yang ◽  
Jean Gan ◽  
Xiaoling Xie ◽  
Min Deng ◽  
Liang Feng ◽  
...  
Keyword(s):  
Hair Cell ◽  
Inner Ear ◽  
Gene Deletion ◽  
Specific Gene ◽  
Mouse Line

scholarly journals Generation of mRx-Cre Transgenic Mouse Line for Efficient Conditional Gene Deletion in Early Retinal Progenitors

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e63029 ◽  
Author(s):  
Lucie Klimova ◽  
Jitka Lachova ◽  
Ondrej Machon ◽  
Radislav Sedlacek ◽  
Zbynek Kozmik
Keyword(s):  
Transgenic Mouse ◽  
Gene Deletion ◽  
Mouse Line ◽  
Transgenic Mouse Line

scholarly journals A mouse model for adult cardiac-specific gene deletion with CRISPR/Cas9

2015 ◽  
Vol 113 (2) ◽  
pp. 338-343 ◽  
Author(s):  
Kelli J. Carroll ◽  
Catherine A. Makarewich ◽  
John McAnally ◽  
Douglas M. Anderson ◽  
Lorena Zentilin ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Gene Deletion ◽  
Disease Modeling ◽  
Embryonic Lethality ◽  
Null Alleles ◽  
Specific Gene ◽  
Specific Expression ◽  
Guide Rna ◽  
Cardiac Gene ◽  
Genomic Editing

Clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas)9 genomic editing has revolutionized the generation of mutant animals by simplifying the creation of null alleles in virtually any organism. However, most current approaches with this method require zygote injection, making it difficult to assess the adult, tissue-specific functions of genes that are widely expressed or which cause embryonic lethality when mutated. Here, we describe the generation of cardiac-specific Cas9 transgenic mice, which express high levels of Cas9 in the heart, but display no overt defects. In proof-of-concept experiments, we used Adeno-Associated Virus 9 (AAV9) to deliver single-guide RNA (sgRNA) that targets the Myh6 locus exclusively in cardiomyocytes. Intraperitoneal injection of postnatal cardiac-Cas9 transgenic mice with AAV9 encoding sgRNA against Myh6 resulted in robust editing of the Myh6 locus. These mice displayed severe cardiomyopathy and loss of cardiac function, with elevation of several markers of heart failure, confirming the effectiveness of this method of adult cardiac gene deletion. Mice with cardiac-specific expression of Cas9 provide a tool that will allow rapid and accurate deletion of genes following a single injection of AAV9-sgRNAs, thereby circumventing embryonic lethality. This method will be useful for disease modeling and provides a means of rapidly editing genes of interest in the heart.

scholarly journals A glycine transporter 2-Cre knock-in mouse line for glycinergic neuron-specific gene manipulation

IBRO Reports ◽  
2017 ◽  
Vol 3 ◽  
pp. 9-16 ◽  
Author(s):  
Toshikazu Kakizaki ◽  
Hiroyuki Sakagami ◽  
Kenji Sakimura ◽  
Yuchio Yanagawa
Keyword(s):  
Specific Gene ◽  
Mouse Line ◽  
Gene Manipulation ◽  
Glycine Transporter ◽  
Glycine Transporter 2

scholarly journals Leptin-dependent serotonin control of appetite: temporal specificity, transcriptional regulation, and therapeutic implications

2010 ◽  
Vol 208 (1) ◽  
pp. 41-52 ◽  
Author(s):  
Vijay K. Yadav ◽  
Franck Oury ◽  
Kenji F. Tanaka ◽  
Tiffany Thomas ◽  
Ying Wang ◽  
...  
Keyword(s):  
Food Intake ◽  
Gene Deletion ◽  
Selective Inhibition ◽  
Specific Gene ◽  
Serotonin Synthesis ◽  
Viable Option ◽  
Therapeutic Implications ◽  
Temporal Specificity ◽  
Specific Antagonist ◽  
Arcuate Nuclei

Recent evidence indicates that leptin regulates appetite and energy expenditure, at least in part by inhibiting serotonin synthesis and release from brainstem neurons. To demonstrate that this pathway works postnatally, we used a conditional, brainstem-specific mouse CreERT2 driver to show that leptin signals in brainstem neurons after birth to decrease appetite by inhibiting serotonin synthesis. Cell-specific gene deletion experiments and intracerebroventricular leptin infusions reveal that serotonin signals in arcuate nuclei of the hypothalamus through the Htr1a receptor to favor food intake and that this serotonin function requires the expression of Creb, which regulates the expression of several genes affecting appetite. Accordingly, a specific antagonist of the Htr1a receptor decreases food intake in leptin-deficient but not in Htr1a−/− mice. Collectively, these results establish that leptin inhibition of serotonin is necessary to inhibit appetite postnatally and provide a proof of principle that selective inhibition of this pathway may be a viable option to treat appetite disorders.

scholarly journals Renal Dysfunction Induced by Kidney-Specific Gene Deletion of Hsd11b2 as a Primary Cause of Salt-Dependent Hypertension

Hypertension ◽  
2017 ◽  
Vol 70 (1) ◽  
pp. 111-118 ◽  
Author(s):  
Kohei Ueda ◽  
Mitsuhiro Nishimoto ◽  
Daigoro Hirohama ◽  
Nobuhiro Ayuzawa ◽  
Wakako Kawarazaki ◽  
...  
Keyword(s):  
Renal Dysfunction ◽  
Gene Deletion ◽  
Specific Gene

Nociceptor-specific gene deletion using heterozygous NaV1.8-Cre recombinase mice

Pain ◽  
2005 ◽  
Vol 113 (1) ◽  
pp. 27-36 ◽  
Author(s):  
Caroline L. Stirling ◽  
Greta Forlani ◽  
Mark D. Baker ◽  
John N. Wood ◽  
Elizabeth A. Matthews ◽  
...  
Keyword(s):  
Gene Deletion ◽  
Cre Recombinase ◽  
Specific Gene

Rhodopsin-iCre transgenic mouse line for Cre-mediated rod-specific gene targeting

genesis ◽  
2005 ◽  
Vol 41 (2) ◽  
pp. 73-80 ◽  
Author(s):  
Sha Li ◽  
Desheng Chen ◽  
Yves Sauvé ◽  
Jeremy McCandless ◽  
Yu-Jiun Chen ◽  
...  
Keyword(s):  
Gene Targeting ◽  
Transgenic Mouse ◽  
Specific Gene ◽  
Mouse Line ◽  
Transgenic Mouse Line
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