scholarly journals Generation of mouse conditional and null alleles of the type III sodium-dependent phosphate cotransporter PiT-1

genesis ◽  
2009 ◽  
pp. NA-NA ◽  
Author(s):  
Maria H. Festing ◽  
Mei Y. Speer ◽  
Hsueh-Ying Yang ◽  
Cecilia M. Giachelli
Keyword(s):  
Null Alleles ◽  
Type Iii ◽  
Sodium Dependent

Phosphaturic action of fibroblast growth factor 23 in Npt2 null mice

2010 ◽  
Vol 298 (6) ◽  
pp. F1341-F1350 ◽  
Author(s):  
Yuka Tomoe ◽  
Hiroko Segawa ◽  
Kazuyo Shiozawa ◽  
Ichiro Kaneko ◽  
Rieko Tominaga ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth Factor 23 ◽  
Fibroblast Growth ◽  
Transport Activity ◽  
Additional Increase ◽  
Type Iii ◽  
Protein Levels ◽  
Sodium Dependent ◽  
Marked Suppression

In the present study, we evaluated the roles of type II and type III sodium-dependent Pi cotransporters in fibroblast growth factor 23 (FGF23) activity by administering a vector encoding FGF23 with the R179Q mutation (FGF23M) to wild-type (WT) mice, Npt2a knockout (KO) mice, Npt2c KO mice, and Npt2a−/−Npt2c−/− mice (DKO mice). In Npt2a KO mice, FGF23M induced severe hypophosphatemia and markedly decreased the levels of Npt2c, type III Na-dependent Pi transporter (PiT2) protein, and renal Na/Pi transport activity. In contrast, in Npt2c KO mice, FGF23M decreased plasma phosphate levels comparable to those in FGF23M-injected WT mice. In DKO mice with severe hypophosphatemia, FGF23M administration did not induce an additional increase in urinary phosphate excretion. FGF23 administration significantly decreased intestinal Npt2b protein levels in WT mice but had no effect in Npt2a, Npt2c, and DKO mice, despite marked suppression of plasma 1,25(OH)2D3 levels in all the mutant mice. The main findings were as follow: 1) FGF23-dependent phosphaturic activity in Npt2a KO mice is dependent on renal Npt2c and PiT-2 protein; 2) in DKO mice, renal Pi reabsorption is not further decreased by FGF23M, but renal vitamin D synthesis is suppressed; and 3) downregulation of intestinal Npt2b may be mediated by a factor(s) other than 1,25(OH)2D3. These findings suggest that Npt2a, Npt2c, and PiT-2 are necessary for the phosphaturic activity of FGF23. Thus complementary regulation of Npt2 family proteins may be involved in systemic Pi homeostasis.

The type III transporters (PiT-1 and PiT-2) are the major sodium-dependent phosphate transporters in the mice and human brains

2016 ◽  
Vol 1637 ◽  
pp. 128-136 ◽  
Author(s):  
Masatoshi Inden ◽  
Masaki Iriyama ◽  
Miho Zennami ◽  
Shin-ichiro Sekine ◽  
Akira Hara ◽  
...  
Keyword(s):  
Phosphate Transporters ◽  
Type Iii ◽  
Sodium Dependent ◽  
Human Brains

scholarly journals The Central Half of Pit2 Is Not Required for Its Function as a Retroviral Receptor

2004 ◽  
Vol 78 (17) ◽  
pp. 9564-9567 ◽  
Author(s):  
Pernille Bøttger ◽  
Lene Pedersen
Keyword(s):  
Amino Acids ◽  
Murine Leukemia Virus ◽  
Deletion Mutant ◽  
Leukemia Virus ◽  
Receptor Function ◽  
Type Iii ◽  
Amphotropic Murine Leukemia Virus ◽  
Sodium Dependent ◽  
Retroviral Receptor ◽  
Murine Leukemia

ABSTRACT The type III sodium-dependent phosphate (NaPi) cotransporter, Pit2, is a receptor for amphotropic murine leukemia virus (A-MuLV) and 10A1 MuLV. In order to determine what is sufficient for Pit2 receptor function, a deletion mutant lacking about the middle half of the protein was made. The mutant supported entry for both viruses, unequivocally narrowing down the identification of the sequence that is sufficient to specify the receptor functions of Pit2 to its N-terminal 182 amino acids and C-terminal 170 amino acids.

scholarly journals Cellular ATP Synthesis Mediated by Type III Sodium-dependent Phosphate TransporterPit-1Is Critical to Chondrogenesis

2010 ◽  
Vol 286 (4) ◽  
pp. 3094-3103 ◽  
Author(s):  
Atsushi Sugita ◽  
Shinji Kawai ◽  
Tetsuyuki Hayashibara ◽  
Atsuo Amano ◽  
Takashi Ooshima ◽  
...  
Keyword(s):  
Atp Synthesis ◽  
Type Iii ◽  
Sodium Dependent
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