Developmentally regulated expression of fibroblast growth factor receptor genes and splice variants by murine embryonic stem and embryonal carcinoma cells

1994 ◽  
Vol 15 (2) ◽  
pp. 148-154 ◽  
Author(s):  
Fiona J. McDonald ◽  
John K. Heath
Keyword(s):  
Embryonal Carcinoma ◽  
Fibroblast Growth Factor Receptor ◽  
Splice Variants ◽  
Embryonic Stem ◽  
Growth Factor Receptor ◽  
Carcinoma Cells ◽  
Fibroblast Growth ◽  
Embryonal Carcinoma Cells ◽  
Developmentally Regulated ◽  
Regulated Expression

Interaction between a novel F9-specific factor and octamer-binding proteins is required for cell-type-restricted activity of the fibroblast growth factor 4 enhancer

1994 ◽  
Vol 14 (12) ◽  
pp. 7758-7769
Author(s):  
L Dailey ◽  
H Yuan ◽  
C Basilico
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Binding Proteins ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Specific Factor ◽  
Fibroblast Growth ◽  
Embryonal Carcinoma Cells ◽  
Enhancer Function ◽  
Restricted Activity

Understanding how diverse transcription patterns are achieved through common factor binding elements is a fundamental question that underlies much of developmental and cellular biology. One example is provided by the fibroblast growth factor 4 (FGF-4) gene, whose expression is restricted to specific embryonic tissues during development and to undifferentiated embryonal carcinoma cells in tissue culture. Analysis of the cis- and trans-acting elements required for the activity of the previously identified FGF-4 enhancer in F9 embryonal carcinoma cells showed that enhancer function depends on sequences that bind Sp1 and ubiquitous as well as F9-specific octamer-binding proteins. However, sequences immediately upstream of the octamer motif, which conform to a binding site for the high-mobility group (HMG) domain factor family, were also critical to enhancer function. We have identified a novel F9-specific factor, Fx, which specifically recognizes this motif. Fx formed complexes with either Oct-1 or Oct-3 in a template-dependent manner. The ability of different enhancer variants to form the Oct-Fx complexes correlated with enhancer activity, indicating that these complexes play an essential role in transcriptional activation of the FGF-4 gene. Thus, while FGF-4 enhancer function is octamer site dependent, its developmentally restricted activity is determined by the interaction of octamer-binding proteins with the tissue-specific factor Fx.

scholarly journals Regulated expression of an introduced MHC H–2Kbm1 gene in murine embryonal carcinoma cells

Nature ◽  
1984 ◽  
Vol 310 (5976) ◽  
pp. 415-418 ◽  
Author(s):  
Arnon Rosenthal ◽  
Stephanie Wright ◽  
Howard Cedar ◽  
Richard Flavell ◽  
Frank Grosveld
Keyword(s):  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Regulated Expression

scholarly journals Comparative proteomics of human embryonic stem cells and embryonal carcinoma cells

PROTEOMICS ◽  
2010 ◽  
Vol 10 (7) ◽  
pp. 1359-1373 ◽  
Author(s):  
Raghothama Chaerkady ◽  
Candace L. Kerr ◽  
Kumaran Kandasamy ◽  
Arivusudar Marimuthu ◽  
John D. Gearhart ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonal Carcinoma ◽  
Embryonic Stem ◽  
Comparative Proteomics ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells
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