Regulated expression of genes encoding soybean β-conglycinins in transgenic plants

1989 ◽  
Vol 10 (2) ◽  
pp. 112-122 ◽  
Author(s):  
Zhang-Liang Chen ◽  
Satoshi Naito ◽  
Ikuo Nakamura ◽  
Roger N. Beachy
Keyword(s):  
Transgenic Plants ◽  
Expression Of Genes ◽  
Regulated Expression ◽  
Genes Encoding

Anther-specific, developmentally regulated expression of genes encoding a new class of proline-rich proteins in sunflower

1991 ◽  
Vol 16 (2) ◽  
pp. 271-281 ◽  
Author(s):  
Jean-Luc Evrard ◽  
Colette Jako ◽  
Agn�s Saint-Guily ◽  
Jacques-Henry Weil ◽  
Marcel Kuntz
Keyword(s):  
Developmentally Regulated ◽  
New Class ◽  
Expression Of Genes ◽  
Regulated Expression ◽  
Genes Encoding

scholarly journals A time course transcriptomic analysis of host and injected oncogenic cells reveals new aspects of Drosophila immune defenses

2021 ◽  
Vol 118 (12) ◽  
pp. e2100825118
Author(s):  
Di Chen ◽  
Arghyashree Roychowdhury-Sinha ◽  
Pragya Prakash ◽  
Xiao Lan ◽  
Wenmin Fan ◽  
...  
Keyword(s):  
Time Course ◽  
Adult Males ◽  
Transcriptomic Response ◽  
Expression Of Genes ◽  
Regulated Expression ◽  
Genes Encoding ◽  
Lag Period ◽  
Induced Genes ◽  
As Stress ◽  
Kinetics Of

Oncogenic RasV12 cells [A. Simcox et al., PLoS Genet. 4, e1000142 (2008)] injected into adult males proliferated massively after a lag period of several days, and led to the demise of the flies after 2 to 3 wk. The injection induced an early massive transcriptomic response that, unexpectedly, included more than 100 genes encoding chemoreceptors of various families. The kinetics of induction and the identities of the induced genes differed markedly from the responses generated by injections of microbes. Subsequently, hundreds of genes were up-regulated, attesting to intense catabolic activities in the flies, active tracheogenesis, and cuticulogenesis, as well as stress and inflammation-type responses. At 11 d after the injections, GFP-positive oncogenic cells isolated from the host flies exhibited a markedly different transcriptomic profile from that of the host and distinct from that at the time of their injection, including in particular up-regulated expression of genes typical for cells engaged in the classical antimicrobial response of Drosophila.

scholarly journals CIPK11: a calcineurin B-like protein-interacting protein kinase from Nitraria tangutorum, confers tolerance to salt and drought in Arabidopsis

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Lu Lu ◽  
Xinying Chen ◽  
Pengkai Wang ◽  
Ye Lu ◽  
Jingbo Zhang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Drought Stress ◽  
Transgenic Plants ◽  
Drought Treatment ◽  
Interacting Protein ◽  
Expression Of Genes ◽  
Nitraria Tangutorum ◽  
Genes Encoding ◽  
Salt And Drought Stress ◽  
Weak Expression

Abstract Background The CIPKs are a group of plant-specific Ser/Thr protein kinases acting in response to calcium signaling, which plays an important role in the physiological and developmental adaptation of plants to adverse environments. However, the functions of halophyte-derived CIPKs are still poorly understood, that limits a potential application of CIPKs from halophytes for improving the tolerance of glycophytes to abiotic stresses. Results In this study, we characterized the NtCIPK11 gene from the halophyte Nitraria tangutorum and subsequently analyzed its role in salt and drought stress tolerance, using Arabidopsis as a transgenic model system. NtCIPK11 expression was upregulated in N. tangutorum root, stem and blade tissues after salt or drought treatment. Overexpressing NtCIPK11 in Arabidopsis improved seed germination on medium containing different levels of NaCl. Moreover, the transgenic plants grew more vigorously under salt stress and developed longer roots under salt or drought conditions than the WT plants. Furthermore, NtCIPK11 overexpression altered the transcription of genes encoding key enzymes involved in proline metabolism in Arabidopsis exposed to salinity, however, which genes showed a relatively weak expression in the transgenic Arabidopsis undergoing mannitol treatment, a situation that mimics drought stress. Besides, the proline significantly accumulated in NtCIPK11-overexpressing plants compared with WT under NaCl treatment, but that was not observed in the transgenic plants under drought stress caused by mannitol application. Conclusions We conclude that NtCIPK11 promotes plant growth and mitigates damage associated with salt stress by regulating the expression of genes controlling proline accumulation. These results extend our understanding on the function of halophyte-derived CIPK genes and suggest that NtCIPK11 can serve as a candidate gene for improving the salt and drought tolerance of glycophytes through genetic engineering.

scholarly journals The Adjustment of Membrane Lipid Metabolism Pathways in Maize Roots Under Saline–Alkaline Stress

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiaoxuan Xu ◽  
Jinjie Zhang ◽  
Bowei Yan ◽  
Yulei Wei ◽  
Shengnan Ge ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Stress Responses ◽  
Membrane Lipids ◽  
Membrane Lipid ◽  
Alkaline Stress ◽  
Expression Of Genes ◽  
Phosphatidic Acid Phosphatase ◽  
Maize Roots ◽  
Regulated Expression ◽  
Genes Encoding

Plants are frequently confronted by diverse environmental stress, and the membrane lipids remodeling and signaling are essential for modulating the stress responses. Saline–alkaline stress is a major osmotic stress affecting the growth and development of crops. In this study, an integrated transcriptomic and lipidomic analysis was performed, and the metabolic changes of membrane lipid metabolism in maize (Zea mays) roots under saline–alkaline stress were investigated. The results revealed that phospholipids were major membrane lipids in maize roots, and phosphatidylcholine (PC) accounts for approximately 40% of the total lipids. Under 100 mmol NaHCO3 treatment, the level of PC decreased significantly (11–16%) and the parallel transcriptomic analysis showed an increased expression of genes encoding phospholipase A and phospholipase D/non-specific phospholipase C, which suggested an activated PC turnover under saline–alkaline stress. The plastidic galactolipid synthesis was also activated, and an abnormal generation of C34:6 galactolipids in 18:3 plants maize implied a plausible contribution from the prokaryotic pathway, which could be partially supported by the up-regulated expression of three putative plastid-localized phosphatidic acid phosphatase/lipid phosphate phosphatase. A comprehensive gene–metabolite network was constructed, and the regulation of membrane lipid metabolism under saline–alkaline stress in maize was discussed.

scholarly journals Deciphering the Regulatory Circuitry That Controls Reversible Lysine Acetylation in Salmonella enterica

mBio ◽  
2015 ◽  
Vol 6 (4) ◽  
Author(s):  
Kristy L. Hentchel ◽  
Sandy Thao ◽  
Peter J. Intile ◽  
Jorge C. Escalante-Semerena
Keyword(s):  
Mutant Strain ◽  
Salmonella Enterica ◽  
Growth Defect ◽  
Metabolic Enzyme ◽  
Transcriptional Level ◽  
Lysine Acetylation ◽  
Content Type ◽  
Expression Of Genes ◽  
Regulated Expression ◽  
Genes Encoding

ABSTRACTInSalmonella enterica, the reversible lysine acetylation (RLA) system is comprised of the protein acetyltransferase (Pat) and sirtuin deacetylase (CobB). RLA controls the activities of many proteins, including the acetyl coenzyme A (acetyl-CoA) synthetase (Acs), by modulating the degree of Acs acetylation. We report that IolR, amyo-inositol catabolism repressor, activates the expression of genes encoding components of the RLA system.In vitroevidence shows that the IolR protein directly regulatespatexpression. AniolRmutant strain displayed a growth defect in minimal medium containing 10 mM acetate, a condition under which RLA function is critical to control Acs activity. Increased levels of Pat, CobB, or Acs activity reversed the growth defect, suggesting the Pat/CobB ratio in aniolRstrain is altered and that such a change affects the level of acetylated, inactive Acs. Results of quantitative reverse transcription-PCR (qRT-PCR) analyses ofpat,cobB, andacsexpression indicated that expression of the genes alluded to in the IolR-deficient strain was reduced 5-, 3-, and 2.6-fold, respectively, relative to the levels present in the strain carrying theiolR+allele. Acs activity in cell-free extracts from aniolRmutant strain was reduced ~25% relative to that of theiolR+strain. Glucose differentially regulated expression ofpat,cobB, andacs. The catabolite repressor protein (Crp) positively regulated expression ofpatwhile having no effect oncobB.IMPORTANCEReversible lysine acylation is used by cells of all domains of life to modulate the function of proteins involved in diverse cellular processes. Work reported herein begins to outline the regulatory circuitry that integrates the expression of genes encoding enzymes that control the activity of a central metabolic enzyme in C2 metabolism. Genetic analyses revealed effects on reversible lysine acylation that greatly impacted the growth behavior of the cell. This work provides the first insights into the complexities of the system responsible for controlling reversible lysine acylation at the transcriptional level in the enteropathogenic bacteriumSalmonella enterica.

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