The effects of proliferation and DNA damage on hematopoietic stem cell function determine aging

2016 ◽  
Vol 245 (7) ◽  
pp. 739-750 ◽  
Author(s):  
Satish Khurana
Keyword(s):  
Dna Damage ◽  
Stem Cell ◽  
Hematopoietic Stem Cell ◽  
Cell Function ◽  
Hematopoietic Stem

scholarly journals Precise Gene Editing Preserves Hematopoietic Stem Cell Function following Transient p53-Mediated DNA Damage Response

2019 ◽  
Vol 24 (4) ◽  
pp. 551-565.e8 ◽  
Author(s):  
Giulia Schiroli ◽  
Anastasia Conti ◽  
Samuele Ferrari ◽  
Lucrezia della Volpe ◽  
Aurelien Jacob ◽  
...  
Keyword(s):  
Dna Damage ◽  
Stem Cell ◽  
Hematopoietic Stem Cell ◽  
Dna Damage Response ◽  
Gene Editing ◽  
Cell Function ◽  
Hematopoietic Stem ◽  
Damage Response

scholarly journals Persistent DNA damage–induced NLRP12 improves hematopoietic stem cell function

JCI Insight ◽  
2020 ◽  
Vol 5 (10) ◽  
Author(s):  
Qiqi Lin ◽  
Limei Wu ◽  
Zhilin Ma ◽  
Fabliha Ahmed Chowdhury,1 ◽  
Habibul Hasan Mazumder ◽  
...  
Keyword(s):  
Dna Damage ◽  
Stem Cell ◽  
Hematopoietic Stem Cell ◽  
Cell Function ◽  
Hematopoietic Stem

JAK2 impairs stem cell function?

Blood ◽  
2010 ◽  
Vol 116 (9) ◽  
pp. 1392-1393 ◽  
Author(s):  
Radek C. Skoda
Keyword(s):  
Dna Damage ◽  
Stem Cell ◽  
Mouse Model ◽  
Hematopoietic Stem Cell ◽  
Essential Thrombocythemia ◽  
Cell Function ◽  
Jak2 V617f ◽  
Hematopoietic Stem

In this issue of Blood, Li et al report that JAK2-V617F increases DNA damage and impairs hematopoietic stem cell function in a conditional knock-in mouse model of JAK2-V617F–positive essential thrombocythemia.1

scholarly journals Prostaglandin E2 Enhances Aged Hematopoietic Stem Cell Function

2021 ◽  
Author(s):  
Andrea M. Patterson ◽  
P. Artur Plett ◽  
Carol H. Sampson ◽  
Edward Simpson ◽  
Yunlong Liu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Prostaglandin E2 ◽  
Hematopoietic Stem Cell ◽  
Cell Function ◽  
Hematopoietic Stem

scholarly journals Hematopoietic Stem Cell Function in a Murine Model of Sickle Cell Disease

Anemia ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Elisabeth H. Javazon ◽  
Mohamed Radhi ◽  
Bagirath Gangadharan ◽  
Jennifer Perry ◽  
David R. Archer
Keyword(s):  
Cell Cycle ◽  
Lipid Peroxidation ◽  
Bone Marrow ◽  
Stem Cell ◽  
Sickle Cell Disease ◽  
Hematopoietic Stem Cell ◽  
Cell Function ◽  
Cell Disease ◽  
Hematopoietic Progenitor ◽  
Hematopoietic Stem

Previous studies have shown that the sickle environment is highly enriched for reactive oxygen species (ROS). We examined the oxidative effects of sickle cell disease on hematopoietic stem cell function in a sickle mouse model.In vitrocolony-forming assays showed a significant decrease in progenitor colony formation derived from sickle compared to control bone marrow (BM). Sickle BM possessed a significant decrease in the KSL (c-kit+, Sca-1+, Lineage−) progenitor population, and cell cycle analysis showed that there were fewer KSL cells in the G0phase of the cell cycle compared to controls. We found a significant increase in both lipid peroxidation and ROS in sickle-derived KSL cells.In vivoanalysis demonstrated that normal bone marrow cells engraft with increased frequency into sickle mice compared to control mice. Hematopoietic progenitor cells derived from sickle mice, however, demonstrated significant impairment in engraftment potential. We observed partial restoration of engraftment by n-acetyl cysteine (NAC) treatment of KSL cells prior to transplantation. Increased intracellular ROS and lipid peroxidation combined with improvement in engraftment following NAC treatment suggests that an altered redox environment in sickle mice affects hematopoietic progenitor and stem cell function.

Loss of FancC Function Results in Decreased Hematopoietic Stem Cell Repopulating Ability

Blood ◽  
1999 ◽  
Vol 94 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Laura S. Haneline ◽  
Troy A. Gobbett ◽  
Rema Ramani ◽  
Madeleine Carreau ◽  
Manuel Buchwald ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hematopoietic Stem Cell ◽  
Cell Function ◽  
Genetic Disorder ◽  
Test Cell ◽  
Hematopoietic Stem ◽  
Murine Homologue ◽  
Complex Genetic Disorder

Fanconi anemia (FA) is a complex genetic disorder characterized by progressive bone marrow (BM) aplasia, chromosomal instability, and acquisition of malignancies, particularly myeloid leukemia. We used a murine model containing a disruption of the murine homologue ofFANCC (FancC) to evaluate short- and long-term multilineage repopulating ability of FancC −/− cells in vivo. Competitive repopulation assays were conducted where “test”FancC −/− or FancC +/+ BM cells (expressing CD45.2) were cotransplanted with congenic competitor cells (expressing CD45.1) into irradiated mice. In two independent experiments, we determined that FancC −/− BM cells have a profound decrease in short-term, as well as long-term, multilineage repopulating ability. To determine quantitatively the relative production of progeny cells by each test cell population, we calculated test cell contribution to chimerism as compared with 1 × 105 competitor cells. We determined that FancC −/− cells have a 7-fold to 12-fold decrease in repopulating ability compared with FancC +/+cells. These data indicate that loss of FancC function results in reduced in vivo repopulating ability of pluripotential hematopoietic stem cells, which may play a role in the development of the BM failure in FA patients. This model system provides a powerful tool for evaluation of experimental therapeutics on hematopoietic stem cell function.

scholarly journals 193Normal hematopoietic stem cell function in mice following treatment with bortezomib

2003 ◽  
Vol 9 (2) ◽  
pp. 121 ◽  
Author(s):  
M. Pitzgerald ◽  
C. Fraser ◽  
I. Webb ◽  
D. Schenkein ◽  
D. Esseltine ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hematopoietic Stem Cell ◽  
Cell Function ◽  
Hematopoietic Stem
Sign in / Sign up

Export Citation Format

Share Document