scholarly journals Zebrafishjam-b2Gal4-enhancer trap line recapitulates endogenousjam-b2expression in extraocular muscles

2015 ◽  
Vol 244 (12) ◽  
pp. 1574-1580 ◽  
Author(s):  
Hideaki Matsui ◽  
Alessandro Dorigo ◽  
Astrid Buchberger ◽  
Jennifer C. Hocking ◽  
Martin Distel ◽  
...  
Keyword(s):  
Extraocular Muscles ◽  
Enhancer Trap ◽  
Enhancer Trap Line ◽  
Trap Line

scholarly journals Zebrafish enhancer trap line showing maternal and neural expression of kctd15a

2012 ◽  
Vol 54 (2) ◽  
pp. 241-252 ◽  
Author(s):  
Naouel Gharbi ◽  
Xiao-Feng Zhao ◽  
Staale Ellingsen ◽  
Anders Fjose
Keyword(s):  
Enhancer Trap ◽  
Enhancer Trap Line ◽  
Trap Line

scholarly journals Molecular identification of a root apical cell-specific and stress-responsive enhancer from an Arabidopsis enhancer trap line

Plant Methods ◽  
2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Lei Zhang ◽  
Li-Na Qin ◽  
Zi-Rui Zeng ◽  
Chang-Zheng Wu ◽  
Yuan-Yong Gong ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Apical Cell ◽  
Enhancer Trap ◽  
Enhancer Trap Line ◽  
Trap Line

scholarly journals An Enhancer Trap Line Associated with a D-Class Cyclin Gene in Arabidopsis

2000 ◽  
Vol 124 (4) ◽  
pp. 1658-1667 ◽  
Author(s):  
Kankshita Swaminathan ◽  
Yingzhen Yang ◽  
Natasha Grotz ◽  
Lauren Campisi ◽  
Thomas Jack
Keyword(s):  
Enhancer Trap ◽  
Enhancer Trap Line ◽  
Cyclin Gene ◽  
Trap Line

scholarly journals The GAL4 enhancer-trap line for analysis of definitive hematopoiesis in zebrafish

2015 ◽  
Vol 19 (2) ◽  
pp. 96-100 ◽  
Author(s):  
Hee Jeong Kong ◽  
Jae-Ho Ryu ◽  
Woo-Jin Kim ◽  
Cheul Min An ◽  
Kyung-Eun Lim ◽  
...  
Keyword(s):  
Enhancer Trap ◽  
Enhancer Trap Line ◽  
Trap Line ◽  
Definitive Hematopoiesis

scholarly journals A zebrafish SKIV2L2-enhancer trap line provides a useful tool for the study of peripheral sensory circuit development

2011 ◽  
Vol 11 (7) ◽  
pp. 409-414 ◽  
Author(s):  
Jane A. Cox ◽  
Anthony R. McAdow ◽  
Amy E. Dinitz ◽  
Andrew S. McCallion ◽  
Stephen L. Johnson ◽  
...  
Keyword(s):  
Enhancer Trap ◽  
Enhancer Trap Line ◽  
Circuit Development ◽  
Peripheral Sensory ◽  
Trap Line

An enhancer trap line identifies the Drosophila homolog of the L37a ribosomal protein

Gene ◽  
1999 ◽  
Vol 239 (1) ◽  
pp. 137-143 ◽  
Author(s):  
Peter Gaines ◽  
Craig T Woodard ◽  
John R Carlson
Keyword(s):  
Ribosomal Protein ◽  
Enhancer Trap ◽  
Enhancer Trap Line ◽  
Drosophila Homolog ◽  
Trap Line

scholarly journals The bereft Gene, a Potential Target of the Neural Selector Gene cut, Contributes to Bristle Morphogenesis

Genetics ◽  
2002 ◽  
Vol 161 (1) ◽  
pp. 231-247 ◽  
Author(s):  
Kirsten E Hardiman ◽  
Rachel Brewster ◽  
Shaema M Khan ◽  
Monika Deo ◽  
Rolf Bodmer
Keyword(s):  
Chromosomal Location ◽  
Insertion Site ◽  
Protein Product ◽  
Enhancer Trap ◽  
P Element ◽  
Deletion Mutants ◽  
Sensory Organs ◽  
Enhancer Trap Line ◽  
Homeobox Transcription Factor ◽  
Trap Line

Abstract The neural selector gene cut, a homeobox transcription factor, is required for the specification of the correct identity of external (bristle-type) sensory organs in Drosophila. Targets of cut function, however, have not been described. Here, we study bereft (bft) mutants, which exhibit loss or malformation of a majority of the interommatidial bristles of the eye and cause defects in other external sensory organs. These mutants were generated by excising a P element located at chromosomal location 33AB, the enhancer trap line E8-2-46, indicating that a gene near the insertion site is responsible for this phenotype. Similar to the transcripts of the gene nearest to the insertion, reporter gene expression of E8-2-46 coincides with Cut in the support cells of external sensory organs, which secrete the bristle shaft and socket. Although bft transcripts do not obviously code for a protein product, its expression is abolished in bft deletion mutants, and the integrity of the bft locus is required for (interommatidial) bristle morphogenesis. This suggests that disruption of the bft gene is the cause of the observed bristle phenotype. We also sought to determine what factors regulate the expression of bft and the enhancer trap line. The correct specification of individual external sensory organ cells involves not only cut, but also the lineage genes numb and tramtrack. We demonstrate that mutations of these three genes affect the expression levels at the bft locus. Furthermore, cut overexpression is sufficient to induce ectopic bft expression in the PNS and in nonneuronal epidermis. On the basis of these results, we propose that bft acts downstream of cut and tramtrack to implement correct bristle morphogenesis.

Fluorescent protein-based imaging and tissue-specific RNA-seq analysis of Arabidopsis hydathodes

2020 ◽  
Author(s):  
Hiroki Yagi ◽  
Atsushi J Nagano ◽  
Jaewook Kim ◽  
Kentaro Tamura ◽  
Nobuyoshi Mochizuki ◽  
...  
Keyword(s):  
Fluorescent Protein ◽  
Small Cells ◽  
Rna Seq ◽  
Water Release ◽  
Tissue Specific ◽  
Enhancer Trap Line ◽  
Physiological Analysis ◽  
Green Fluorescent ◽  
Trap Line ◽  
Lower Expression

Abstract Hydathodes are typically found at leaf teeth in vascular plants and are involved in water release to the outside. Although morphological and physiological analysis of hydathodes has been performed in various plants, little is known about the genes involved in hydathode function. In this study, we performed fluorescent protein-based imaging and tissue-specific RNA-seq analysis in Arabidopsis hydathodes. We used the enhancer trap line E325, which has been reported to express green fluorescent protein (GFP) at its hydathodes. We found that E325-GFP was expressed in small cells found inside the hydathodes (named E cells) that were distributed between the water pores and xylem ends. No fluorescence of the phloem markers pSUC2:GFP and pSEOR1:SEOR1-YFP was observed in the hydathodes. These observations indicate that Arabidopsis hydathodes are composed of three major components: water pores, xylem ends, and E cells. In addition, we performed transcriptome analysis of the hydathode using the E325-GFP line. Microsamples were collected from GFP-positive or -negative regions of E325 leaf margins with a needle-based device (~130 µm in diameter). RNA-seq was performed with each single microsample using a high-throughput library preparation method called Lasy-Seq. We identified 72 differentially expressed genes. Among them, 68 genes showed significantly higher and four genes showed significantly lower expression in the hydathode. Our results provide new insights into the molecular basis for hydathode physiology and development.

Genetic organization of the ci-M-pan region on chromosome IV in Drosophila melanogaster

Genome ◽  
1999 ◽  
Vol 42 (6) ◽  
pp. 1144-1149
Author(s):  
Jesper Kronhamn ◽  
Åsa Rasmuson-Lestander
Keyword(s):  
Drosophila Melanogaster ◽  
Ribosomal Protein ◽  
P Element ◽  
Gene Encoding ◽  
Regulatory Regions ◽  
Cubitus Interruptus ◽  
First Intron ◽  
Enhancer Trap Line ◽  
Mutant Lines ◽  
Trap Line

The genes cubitus interruptus (ci), ribosomal protein S3A (RpS3A), and pangolin (pan) are localized within 73 kb in the cytological region 101F-102A on chromosome IV in Drosophila melanogaster. A region of 13 kb harbours the regulatory regions of both ci and pan, transcribed in opposite directions, and a 1.1-kb gene encoding RpS3A. This dense clustering gives rise to very complicated complementation patterns between different alleles in these loci. We investigated this region genetically and molecularly by use of an enhancer trap line (IA5), where the P-element was found to be inserted into the first intron of pan. Screens for imprecise excisions of the P-element were performed, and complementations between new and old established mutant lines were investigated. We found that when mutated or deleted the RpS3A gene gives rise to a Minute phenotype, and we conclude that M(4)101 encodes the ribosomal protein S3A.Key words: Drosophila melanogaster, Minute(4)101, ribosomal protein, RpS3A gene, P-element mutagenesis.

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