scholarly journals Identification of germ plasm-associated transcripts by microarray analysis of Xenopus vegetal cortex RNA

2010 ◽  
Vol 239 (6) ◽  
pp. 1838-1848 ◽  
Author(s):  
Tawny N. Cuykendall ◽  
Douglas W. Houston
Development ◽  
1995 ◽  
Vol 121 (1) ◽  
pp. 201-208 ◽  
Author(s):  
C. Forristall ◽  
M. Pondel ◽  
L. Chen ◽  
M.L. King

In Xenopus, localization of a rare class of mRNAs during oogenesis is believed to initiate pattern formation in the early embryo. We have determined the pattern of RNA localization for one of these RNAs, Xcat-2, which encodes a putative RNA-binding protein related to Drosophila nanos (Mosquera, L., Forristall, C., Zhou, Y. and King, M. L. (1993) Development 117, 377–386). Xcat-2 is exclusively localized to the mitochondrial cloud in stage I oocytes, moves with this body into the vegetal cortex during stage II and, later, partitions into islands consistent with it being a component of the germ plasm. As previously shown, Vg1 is not localized to the vegetal cortex until stage IV and distributes to all vegetal blastomeres during development. We found a direct correlation between the localized condition of these RNAs and their recovery in a detergent-insoluble fraction. We present evidence suggesting that differential RNA binding to a cytoskeletal component(s) in the vegetal cortex determines the pattern of inheritance for that RNA in the embryo.


2001 ◽  
Vol 120 (5) ◽  
pp. A455-A455
Author(s):  
E MANNICK ◽  
M SERRANO ◽  
J BONOMOLO ◽  
M LAU ◽  
J UDALLJR ◽  
...  

2005 ◽  
Vol 173 (4S) ◽  
pp. 211-211
Author(s):  
Loleta D. Harris ◽  
Tomasz Tuziak ◽  
Jorge De Lo Cerda ◽  
Anita L. Sabichi ◽  
Ying Yang ◽  
...  

2007 ◽  
Author(s):  
Joshua Hunsberger ◽  
Samuel Newton

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