scholarly journals Depigmentation of skin and hair color in the somatic cell cloned pig

2009 ◽  
Vol 238 (7) ◽  
pp. 1701-1708 ◽  
Author(s):  
Kyu-Chan Hwang ◽  
Seong-Keun Cho ◽  
Seong-Hoon Lee ◽  
Jong-Yi Park ◽  
Deug-Nam Kwon ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Hair Color ◽  
Cloned Pig

scholarly journals Biotechnological bases of the development of cloned pig embryos

2019 ◽  
Vol 23 (5) ◽  
pp. 527-533
Author(s):  
A. V. Lopukhov ◽  
G. N. Singina ◽  
N. A. Zinovieva
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Donor Cell ◽  
Oocyte Nucleus ◽  
First Polar Body ◽  
Wide Range ◽  
Micro Tools ◽  
Cloned Pig

The term ‘clone’ in animal biotechnology refers to an organism derived from non-sexual reproduction, which is both a direct offspring and a genetic copy of the parent organism. To date, the pig appears to be the most interesting object in cloning research. Somatic cell nuclear transfer in pigs has a wide range of potential applications in various fields of human scientific and economic activities. However, the efficiency of producing cloned embryos in swine is still lower than that of other livestock species, in particular horses and cattle. Somatic cell nuclear transfer is a technically complex multi-stage technology, at each stage of which the pig oocytes, which are more susceptible to changes of surrounding conditions, are affected by various factors (mechanical, physical, chemical). At the stage of oocyte maturation, changes in the cell ultrastructures of the ooplasm occur, which play an important role in the subsequent nuclear reprogramming of the transferred donor cell. Before transfer to the oocyte donor somatic cells are synchronized in the G0/G1 stage of the cell cycle to ensure the normal ploidy of the cloned embryo. When removing the nucleus of pig oocytes maturated in vitro, it is necessary to pay attention to the problem of preserving the viability of cells, which were devoid of their own nuclear material. To perform the reconstruction, a somatic cell is placed, using micro-tools, in the perivitelline space, where the first polar body was previously located, or in the cytoplasm of an enucleated oocyte. The method of manual cloning involves the removal of the oocyte nucleus with subsequent fusion with the donor cell without the use of micromanipulation techniques. The increased sensitivity of oocytes to the environmental conditions causes special requirements for the choice of the system for in vitro culture of cloned pig embryos. In this work, we have reviewed the modern methods used for the production of cloned embryos and identified the technological issues that prevent improving the efficiency of somatic cloning of pigs.

Does a woman's hair color and length affect perceptions and attentional focus?

2014 ◽  
Author(s):  
Susanna S. P. Petaisto ◽  
David C. Matz ◽  
Hillary B. Manning
Keyword(s):  
Attentional Focus ◽  
Hair Color

Looking Out for Something Better to Come: Interview with Director Hanna Polak

2016 ◽  
Vol 69 (4) ◽  
pp. 65-71
Author(s):  
Masha Shpolberg
Keyword(s):  
United States ◽  
The United States ◽  
Hair Color ◽  
Yale University ◽  
Garbage Dump ◽  
To Come

Hanna Polak was in the United States in December 2015 for a screening of Something Better to Come (2014) and The Children of Leningradsky (2004) at Yale University, where the interview was conducted. Polak's devastating documentary Something Better to Come swept through the festival circuit with force, winning a Special Jury Award at IDFA along with awards at over twenty other festivals. Shot illegally on a garbage dump just outside Moscow over the course of fourteen years, the film follows a girl named Yula from age 10 to 24, as she grows up doing the things that teenagers everywhere do—experimenting with her hair color and makeup, with cigarettes and alcohol—all while living in the most difficult of conditions.

Characterization of mesenchymal stem cells and their application in experimental embryology

2013 ◽  
Vol 16 (3) ◽  
pp. 593-599 ◽  
Author(s):  
J. Opiela ◽  
M. Samiec
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Somatic Cell ◽  
Fertilization In Vitro ◽  
Cell Cloning ◽  
Mammalian Embryo ◽  
Vitro Fertilization ◽  
Experimental Embryology ◽  
Genomic Engineering

Abstract The efficiency of somatic cell cloning (somatic cell nuclear transfer; SCNT) as well as in vitro fertilization/in vitro embryo production (IVF/IVP) in mammals stay at relatively same level for over a decade. Despite plenty of different approaches none satisfactory break-through took place. In this article, we briefly summarize the implementation of mesenchymal stem cells (MSCs) for experimental embryology. The advantages of using MSCs as nuclear donors in somatic cell cloning and in vitro embryo culture are described. The description of results obtained with these cells in mammalian embryo genomic engineering is presented.

Effect of somatic cell counts in milk on its quality depending on cow breed and season

2010 ◽  
Vol 28 (3) ◽  
Author(s):  
Jolanta Król ◽  
Aneta Brodziak ◽  
Mariusz Florek ◽  
Zygmunt Litwińczuk
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Counts ◽  
Cell Counts

TOKSISITAS AKUT INFUSA KULIT ARI KACANG TANAH (Arachis hypogea L.) PADA MENCIT BALB/ C

2018 ◽  
Vol 15 (2) ◽  
pp. 62
Author(s):  
Risha Fillah Fithria ◽  
Ririn Lispita Wulandari ◽  
Devi Nisa Hidayati ◽  
Lilis Rejeki
Keyword(s):  
Single Dose ◽  
Color Change ◽  
Negative Control ◽  
Control Group ◽  
Hair Color ◽  
Peanut Shell ◽  
Control Group Design ◽  
Safety Standards ◽  
Arachis Hypogea ◽  
Passive Behavior

ABSTRACTPeanut shell (PS) infusion has been shown to be antithrombocytopenia, but there has been no research on safety standards. This study aims to identify the symptoms of toxic effects, the potency of toxicity and histopathology of liver male Balb/C mice after a single dose of PS infusion. This research uses randomized matched posttest only control group design. Twenty five mice were divided into 5 orally dosage groups, ie, PS infusion with a dose of 0,026; 0.052; 0.104; 0.208 g/20gBW; and negative control of CMC Na 0.5%. The observation period is for 14 days. The results showed that single dose of PS infusion had a pseudo LD50 value ie > 0.208g/20gBW which was practically non toxic. Symptoms to watch out for the BW infusion were passive behavior, bradycnea, hair color change, hair loss, and weight loss at doses of 3 and 4. It is unclear whether liver damage ie inflammation, necrosis, and albuminous degeneration caused by PS infusion or other causes.keywords: acute toxicity, infusion, peanut shell

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