scholarly journals Migratory patterns and developmental potential of trunk neural crest cells in the axolotl embryo

2007 ◽  
Vol 236 (2) ◽  
pp. 389-403 ◽  
Author(s):  
Hans-Henning Epperlein ◽  
Mark A.J. Selleck ◽  
Daniel Meulemans ◽  
Levan Mchedlishvili ◽  
Robert Cerny ◽  
...  
Keyword(s):  
Neural Crest ◽  
Neural Crest Cells ◽  
Developmental Potential ◽  
Trunk Neural Crest ◽  
Migratory Patterns

scholarly journals Migrating neural crest cells in the trunk of the avian embryo are multipotent

Development ◽  
1991 ◽  
Vol 112 (4) ◽  
pp. 913-920 ◽  
Author(s):  
S.E. Fraser ◽  
M. Bronner-Fraser
Keyword(s):  
Neural Crest ◽  
Neural Tube ◽  
Sympathetic Neurons ◽  
Morphological Characteristics ◽  
Neural Crest Cells ◽  
Cell Types ◽  
Sympathetic Ganglia ◽  
Avian Embryo ◽  
Developmental Potential ◽  
Trunk Neural Crest

Trunk neural crest cells migrate extensively and give rise to diverse cell types, including cells of the sensory and autonomic nervous systems. Previously, we demonstrated that many premigratory trunk neural crest cells give rise to descendants with distinct phenotypes in multiple neural crest derivatives. The results are consistent with the idea that neural crest cells are multipotent prior to their emigration from the neural tube and become restricted in phenotype after leaving the neural tube either during their migration or at their sites of localization. Here, we test the developmental potential of migrating trunk neural crest cells by microinjecting a vital dye, lysinated rhodamine dextran (LRD), into individual cells as they migrate through the somite. By two days after injection, the LRD-labelled clones contained from 2 to 67 cells, which were distributed unilaterally in all embryos. Most clones were confined to a single segment, though a few contributed to sympathetic ganglia over two segments. A majority of the clones gave rise to cells in multiple neural crest derivatives. Individual migrating neural crest cells gave rise to both sensory and sympathetic neurons (neurofilament-positive), as well as cells with the morphological characteristics of Schwann cells, and other non-neuronal cells (both neurofilament-negative). Even those clones contributing to only one neural crest derivative often contained both neurofilament-positive and neurofilament-negative cells. Our data demonstrate that migrating trunk neural crest cells can be multipotent, giving rise to cells in multiple neural crest derivatives, and contributing to both neuronal and non-neuronal elements within a given derivative.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Cell lineage analysis of the avian neural crest

Development ◽  
1991 ◽  
Vol 113 (Supplement_2) ◽  
pp. 17-22 ◽  
Author(s):  
Marianne Bronner-Fraser ◽  
Scott E. Fraser
Keyword(s):  
Neural Crest ◽  
Cell Fate ◽  
Neural Tube ◽  
Sympathetic Neurons ◽  
Cell Lineage ◽  
Morphological Characteristics ◽  
Neural Crest Cell ◽  
Neural Crest Cells ◽  
Developmental Potential ◽  
Trunk Neural Crest

Neural crest cells migrate extensively and give rise to diverse cell types, including cells of the sensory and autonomic nervous systems. A major unanswered question concerning the neural crest is when and how the neural crest cells become determined to adopt a particular fate. We have explored the developmental potential of trunk neural crest cells in avian embryos by microinjecting a vital dye, lysinated rhodamine dextran (LRD), into individual cells within the dorsal neural tube. We find that premigratory and emigrating neural crest cells give rise to descendants with distinct phenotypes in multiple neural crest derivatives. These results are consistent with the idea that neural crest cells are multipotent prior to their emigration from the neural tube and become restricted in phenotype after emigration from the neural tube either during their migration or at their sites of localization. To determine whether neural crest cells become restricted during their migration, we have microinjected individual trunk neural crest cells with dye shortly after they leave the neural tube or as they migrate through the somite. We find that a majority of the clones derived from migrating neural crest cells appear to be multipotent; individual migrating neural crest cells gave rise to both sensory and sympathetic neurons, as well as cells with the morphological characteristics of Schwann cells, and other nonneuronal cells. Even those clones contributing to only one neural crest derivative often contained both neurofilament-positive and neurofilament-negative cells. These data demonstrate that migrating trunk neural crest cells, like their premigratory progenitors, can be multipotent. They give rise to cells in multiple neural crest derivatives and contribute to both neuronal and non-neuronal elements within a given derivative. Thus, restriction of neural crest cell fate must occur relatively late in migration or at the final destinations.

Developmental potential of avian trunk neural crest cells in situ

Neuron ◽  
1989 ◽  
Vol 3 (6) ◽  
pp. 755-766 ◽  
Author(s):  
Marianne Bronner-Fraser ◽  
Scott Fraser
Keyword(s):  
Neural Crest ◽  
Neural Crest Cells ◽  
Developmental Potential ◽  
Trunk Neural Crest

scholarly journals Molecular and Cellular Features of Murine Craniofacial and Trunk Neural Crest Cells as Stem Cell-Like Cells

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e84072 ◽  
Author(s):  
Kunie Hagiwara ◽  
Takeshi Obayashi ◽  
Nobuyuki Sakayori ◽  
Emiko Yamanishi ◽  
Ryuhei Hayashi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Neural Crest ◽  
Neural Crest Cells ◽  
Trunk Neural Crest

scholarly journals Characterization of late emerging trunk neural crest cells in the turtle Trachemys scripta

2010 ◽  
Vol 344 (1) ◽  
pp. 531
Author(s):  
Judith A. Cebra-Thomas ◽  
James Robinson ◽  
Melinda Yin ◽  
James McCarthy ◽  
Sonal Shah ◽  
...  
Keyword(s):  
Neural Crest ◽  
Neural Crest Cells ◽  
Trachemys Scripta ◽  
Trunk Neural Crest

scholarly journals Matrigel supports neural, melanocytic and chondrogenic differentiation of trunk neural crest cells

2013 ◽  
Vol 57 (11-12) ◽  
pp. 885-890 ◽  
Author(s):  
Ana B. Ramos-Hryb ◽  
Meline C. Da-Costa ◽  
Andréa G. Trentin ◽  
Giordano W. Calloni
Keyword(s):  
Neural Crest ◽  
Chondrogenic Differentiation ◽  
Neural Crest Cells ◽  
Trunk Neural Crest

The differentiation in vitro of the neural crest cells of the mouse embryo

Development ◽  
1984 ◽  
Vol 84 (1) ◽  
pp. 49-62
Author(s):  
Kazuo Ito ◽  
Takuji Takeuchi
Keyword(s):  
Neural Crest ◽  
Mouse Embryo ◽  
Neural Crest Cells ◽  
Culture Method ◽  
Gene Control ◽  
Developmental Potential ◽  
Neural Tubes ◽  
Epithelial Sheet ◽  
Culture Phase

A culture method for neural crest cells of mouse embryo is described. Trunk neural tubes were dissected from 9-day mouse embryos and explanted in culture dishes. The developmental potential of mouse neural crest in vitro was shown to be essentially similar to that of avian neural crest. In the mouse, however, melanocytes always appeared in association with the epithelial sheet close to the explant. Neural crest cells surrounding the epithelial sheet, which probably migrated from the neural tubes in the early culture phase, never differentiated into melanocytes. The bimodal behaviour of mouse crest cells seems to be due to the heterogenous potency of the crest cells and the interaction of these cells with the surrounding microenvironment. This culture system is well suited for various experiments including the analysis of gene control on the differentiation of neural crest cells.

An SEM analysis of neural crest migration in the mouse

Development ◽  
1983 ◽  
Vol 74 (1) ◽  
pp. 97-118
Author(s):  
C. A. Erickson ◽  
J. A. Weston
Keyword(s):  
Neural Crest ◽  
Basal Lamina ◽  
Neural Tube ◽  
Neural Crest Cells ◽  
Sem Analysis ◽  
Basement Membranes ◽  
Dorsal Neural Tube ◽  
Trunk Neural Crest ◽  
Unique Cell ◽  
Neural Crest Migration

The cellular morphology and migratory pathways of the trunk neural crest are described in normal mouse embryos, and in embryos homozygous for Patch in which neural crest derivatives develop abnormally. Trunk neural crest cells initially appear in 8½-day embryos as a unique cell population on the dorsal neural tube surface and are relatively rounded. Once they begin to migrate the cells flatten and orient somewhat tangentially to the neural tube, and advance ventrad between the somites and neural tube. At the onset of migration neural crest cells extend lamellipodia onto the surface of the tube while detaching their trailing processes from the lumenal surface. The basal lamina on the dorsal neural tube is discontinuous when cell migration begins in this region. As development proceeds, the basal lamina gradually becomes continuous from a lateral to dorsal direction and neural crest emigration is progressively confined to the narrowing region of discontinuous basal lamina. Cell separation from the neural tube ceases concomitant with completion of a continuous basement membrane. Preliminary observations of the mutant embryos reveal that abnormal extracellular spaces appear and patterns of crest migration are subsequently altered. We conclude that the extracellular matrix, extracellular spaces and basement membranes may delimit crest migration in the mouse.

scholarly journals Transcriptome dataset of trunk neural crest cells migrating along the ventral pathway of chick embryos

Data in Brief ◽  
2018 ◽  
Vol 21 ◽  
pp. 2547-2553 ◽  
Author(s):  
Christina Murko ◽  
Felipe Monteleone Vieceli ◽  
Marianne Bronner
Keyword(s):  
Neural Crest ◽  
Neural Crest Cells ◽  
Chick Embryos ◽  
Ventral Pathway ◽  
Trunk Neural Crest
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