Pattern of Pax7 expression during myogenesis in the posthatch chicken establishes a model for satellite cell differentiation and renewal

2004 ◽  
Vol 231 (3) ◽  
pp. 489-502 ◽  
Author(s):  
Orna Halevy ◽  
Yogev Piestun ◽  
Mohammed Z. Allouh ◽  
Benjamin W.C. Rosser ◽  
Yuval Rinkevich ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Satellite Cell

scholarly journals Effects of intramuscular administration of 1α,25(OH)2D3 during skeletal muscle regeneration on regenerative capacity, muscular fibrosis, and angiogenesis

2016 ◽  
Vol 120 (12) ◽  
pp. 1381-1393 ◽  
Author(s):  
Ratchakrit Srikuea ◽  
Muthita Hirunsai
Keyword(s):  
Skeletal Muscle ◽  
Vitamin D ◽  
Protein Synthesis ◽  
Cell Differentiation ◽  
Satellite Cell ◽  
Muscle Regeneration ◽  
Fiber Type ◽  
Fiber Formation ◽  
Skeletal Muscle Regeneration ◽  
Type Composition

The recent discovery of the vitamin D receptor (VDR) in regenerating muscle raises the question regarding the action of vitamin D3 on skeletal muscle regeneration. To investigate the action of vitamin D3 on this process, the tibialis anterior muscle of male C57BL/6 mice (10 wk of age) was injected with 1.2% BaCl2 to induce extensive muscle injury. The bioactive form of vitamin D3 [1α,25(OH)2D3] was administered daily via intramuscular injections during the regenerative phase (days 4-7 postinjury). Physiological and supraphysiological doses of 1α,25(OH)2D3 relative to 1 μg/kg muscle wet weight and mouse body weight were investigated. Muscle samples were collected on day 8 postinjury to examine proteins related to vitamin D3 metabolism (VDR, CYP24A1, and CYP27B1), satellite cell differentiation and regenerative muscle fiber formation [myogenin and embryonic myosin heavy chain (EbMHC)], protein synthesis signaling (Akt, p70 S6K1, 4E-BP1, and myostatin), fiber-type composition (fast and slow MHCs), fibrous formation (vimentin), and angiogenesis (CD31). Administration of 1α,25(OH)2D3 at physiological and supraphysiological doses enhanced VDR expression in regenerative muscle. Moreover, CYP24A1 and vimentin expression was increased, accompanying decreased myogenin and EbMHC expression at the supraphysiological dose. However, there was no change in CYP27B1, Akt, p70 S6K1, 4E-BP1, myostatin, fast and slow MHCs, or CD31 expression at any dose investigated. Taken together, administration of 1α,25(OH)2D3 at a supraphysiological dose decreased satellite cell differentiation, delayed regenerative muscle fiber formation, and increased muscular fibrosis. However, protein synthesis signaling, fiber-type composition, and angiogenesis were not affected by either 1α,25(OH)2D3 administration at a physiological or supraphysiological dose.

Effect ofECM2expression on bovine skeletal muscle-derived satellite cell differentiation

2018 ◽  
Vol 42 (5) ◽  
pp. 525-532 ◽  
Author(s):  
Chang Liu ◽  
Huili Tong ◽  
Shufeng Li ◽  
Yunqin Yan
Keyword(s):  
Skeletal Muscle ◽  
Cell Differentiation ◽  
Satellite Cell ◽  
Bovine Skeletal Muscle

scholarly journals Barx2 and Pax7 Have Antagonistic Functions in Regulation of Wnt Signaling and Satellite Cell Differentiation

Stem Cells ◽  
2014 ◽  
Vol 32 (6) ◽  
pp. 1661-1673 ◽  
Author(s):  
Lizhe Zhuang ◽  
Julie-Ann Hulin ◽  
Anastasia Gromova ◽  
Thi Diem Tran Nguyen ◽  
Ruth T. Yu ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Wnt Signaling ◽  
Satellite Cell

scholarly journals Atypical Protein Kinase Cs Are the Ras Effectors That Mediate Repression of Myogenic Satellite Cell Differentiation

2002 ◽  
Vol 22 (4) ◽  
pp. 1140-1149 ◽  
Author(s):  
Yuri V. Fedorov ◽  
Nathan C. Jones ◽  
Bradley B. Olwin
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Cell Differentiation ◽  
Cell Line ◽  
Satellite Cell ◽  
Myogenic Differentiation ◽  
Ectopic Expression ◽  
Dominant Negative ◽  
Dependent Inhibition ◽  
Ras Effectors

ABSTRACT Oncogenic Ha-Ras is a potent inhibitor of skeletal muscle cell differentiation, yet the Ras effector mediating this process remains unidentified. Here we demonstrate that the atypical protein kinases (aPKCs; λ and/or ζ) are downstream Ras effectors responsible for Ras-dependent inhibition of myogenic differentiation in a satellite cell line. First, ectopic expression of Ha-RasG12V induces translocation of PKCλ from the cytosol to the nucleus, suggesting that aPKCs are activated by Ras in myoblasts. The aPKCs function as downstream Ras effectors since inhibition of aPKCs by expression of a dominant negative PKCζ mutant or by treatment of cells with an inhibitor, GO6983, promotes myogenesis in skeletal muscle satellite cells expressing oncogenic Ha-Ras. Arresting cell proliferation synergistically enhances myogenic differentiation only when aPKCs are also inhibited. Thus, the repression of myogenic differentiation in a satellite cell line appears to be directly mediated by aPKCs acting as Ras effectors and indirectly mediated via stimulation of cell proliferation.

scholarly journals β3‐Integrin mediates satellite cell differentiation in regenerating mouse muscle

2011 ◽  
Vol 25 (6) ◽  
pp. 1914-1921 ◽  
Author(s):  
Huijie Liu ◽  
Airu Niu ◽  
Shuen‐Ei Chen ◽  
Yi‐Ping Li
Keyword(s):  
Cell Differentiation ◽  
Satellite Cell ◽  
Mouse Muscle ◽  
Β3 Integrin

Aging-related satellite cell differentiation defect occurs prematurely after Ski-induced muscle hypertrophy

2002 ◽  
Vol 283 (4) ◽  
pp. C1228-C1241 ◽  
Author(s):  
Sophie B. P. Chargé ◽  
Andrew S. Brack ◽  
Simon M. Hughes
Keyword(s):  
Cell Differentiation ◽  
Satellite Cell ◽  
Satellite Cells ◽  
Genetic Background ◽  
Late Onset ◽  
Early Event ◽  
First Year ◽  
Muscle Aging ◽  
First Year Of Life ◽  
Differentiation Efficiency

To investigate the cause of skeletal muscle weakening during aging we examined the sequence of cellular changes in murine muscles. Satellite cells isolated from single muscle fibers terminally differentiate progressively less well with increasing age of donor. This change is detected before decline in satellite cell numbers and all histological changes examined here. In MSVski transgenic mice, which show type IIb fiber hypertrophy, initial muscle weakness is followed by muscle degeneration in the first year of life. This degeneration is accompanied by a spectrum of changes typical of normal muscle aging and a more marked decline in satellite cell differentiation efficiency. On a myoD-null genetic background, in which satellite cell differentiation is defective, the MSVski muscle phenotype is aggravated. This suggests that, on a wild-type genetic background, satellite cells are capable of repairing MSVski fibers and preserving muscle integrity in early life. We propose that decline in myogenic cell differentiation efficiency is an early event in aging-related loss of muscle function, both in normal aging and in some late-onset muscle degenerative conditions.

Sign in / Sign up

Export Citation Format

Share Document