Spatial and temporal distribution of nerves, ganglia, and smooth muscle during the early pseudoglandular stage of fetal mouse lung development

2001 ◽  
Vol 221 (1) ◽  
pp. 48-60 ◽  
Author(s):  
Jenny Tollet ◽  
Alan W. Everett ◽  
Malcolm P. Sparrow
Keyword(s):  
Smooth Muscle ◽  
Lung Development ◽  
Temporal Distribution ◽  
Mouse Lung ◽  
Spatial And Temporal Distribution ◽  
Fetal Mouse ◽  
Pseudoglandular Stage

scholarly journals Human lung branching morphogenesis is orchestrated by the spatiotemporal distribution of ACTA2, SOX2, and SOX9

2018 ◽  
Vol 314 (1) ◽  
pp. L144-L149 ◽  
Author(s):  
Soula Danopoulos ◽  
Irving Alonso ◽  
Matthew E. Thornton ◽  
Brendan H. Grubbs ◽  
Saverio Bellusci ◽  
...  
Keyword(s):  
Lung Development ◽  
Progenitor Cell ◽  
Human Lung ◽  
Branching Morphogenesis ◽  
Mouse Lung ◽  
Double Positive ◽  
Progenitor Cell Population ◽  
Positive Population ◽  
Pseudoglandular Stage ◽  
Lung Branching

Lung morphogenesis relies on a number of important processes, including proximal-distal patterning, cell proliferation, migration and differentiation, as well as epithelial-mesenchymal interactions. In mouse lung development, SOX2+ cells are localized in the proximal epithelium, whereas SOX9+ cells are present in the distal epithelium. We show that, in human lung, expression of these transcription factors differs, in that during the pseudoglandular stage distal epithelial progenitors at the tips coexpress SOX2 and SOX9. This double-positive population was no longer present by the canalicular stages of development. As in mouse, the human proximal epithelial progenitors express solely SOX2 and are surrounded by smooth muscle cells (SMCs) both in the proximal airways and at the epithelial clefts. Upon Ras-related C3 botulinum toxin substrate 1 inhibition, we noted decreased branching, as well as increased SMC differentiation, attenuated peristalsis, and a reduction in the distal double-positive SOX2/SOX9 progenitor cell population. Thus, the presence of SOX2/SOX9 double-positive progenitor cells in the distal epithelium during the pseudoglandular stage of human lung development appears to be critical to proximal-distal patterning and lung branching. Moreover, SMCs promote a SOX2 proximal phenotype and seem to suppress the SOX9+ population.

scholarly journals Smooth muscle differentiation shapes domain branches during mouse lung development

Development ◽  
2019 ◽  
Vol 146 (22) ◽  
pp. dev181172 ◽  
Author(s):  
Katharine Goodwin ◽  
Sheng Mao ◽  
Tristan Guyomar ◽  
Erin Miller ◽  
Derek C. Radisky ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Lung Development ◽  
Muscle Differentiation ◽  
Mouse Lung ◽  
Smooth Muscle Differentiation

Laminin 2 attachment selects myofibroblasts from fetal mouse lung

1998 ◽  
Vol 275 (3) ◽  
pp. L622-L630
Author(s):  
Guillermo Flores-Delgado ◽  
Pablo Bringas ◽  
David Warburton
Keyword(s):  
Lung Development ◽  
Cell Biology ◽  
Smooth Muscle Actin ◽  
Primary Cultures ◽  
Fetal Lung ◽  
Mesenchymal Cells ◽  
Mouse Lung ◽  
Pcr Analysis ◽  
Rt Pcr ◽  
Fetal Mouse

Laminins (LNs) are extracellular matrix glycoproteins that are involved in cell adhesion, proliferation, and differentiation. So far, 11 LN variants (LN1 to LN11) have been described. In the lung, at least six LN variants have been identified. However, only the role of LN1 has been characterized to any extent. In this study, we hypothesized that the LN2 variant may play a role during lung development. We identified, by RT-PCR analysis, that the α2-chain mRNA of LN2 is expressed during mouse lung development. LN2 adhesion assays were then performed with cells from fetal mouse lung primary cultures. Our results showed that a specific subpopulation of fetal lung cells that expressed vimentin, α-smooth muscle actin, and desmin attached onto LN2, whereas the cells that did not adhere to LN2 as well as the total cell population were able to adhere readily on fibronectin. Cell attachment onto LN2 was inhibited by EDTA. In addition, we demonstrated, by RT-PCR and Western analysis, that the LN2-adherent cells autoexpressed the α2-chain of LN2. In the late pseudoglandular period, LN2 was localized by immunohistochemistry in the basement membrane of airways and blood vessels and around mesenchymal cells. We conclude that LN2 is expressed during lung development and that a specific subpopulation of fetal lung mesenchymal cells expressing a myofibroblastic phenotype can be selected by attachment to LN2 in primary culture. These findings lead us to speculate that LN2 may play a key role in the cell biology of myofibroblasts during lung development.

The role of WNT/b-catenin signaling in smooth muscle cells during lung development and repair

Pneumologie ◽  
2014 ◽  
Vol 68 (06) ◽  
Author(s):  
A Moiseenko ◽  
E El Agha ◽  
B MacKenzie ◽  
S De Langhe ◽  
S Bellusci
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Lung Development ◽  
Muscle Cells
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