Indirect fibre-optic colorimetric determination of ascorbic acid using 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol and cloud point extraction

2011 ◽  
Vol 5 (4) ◽  
pp. 228-233 ◽  
Author(s):  
Hayati Filik ◽  
Derya Giray
Keyword(s):  
Ascorbic Acid ◽  
Cloud Point ◽  
Cloud Point Extraction ◽  
Colorimetric Determination ◽  
Fibre Optic

Utilization of cloud-point extraction for colorimetric determination of trace amounts of thorium(iv) in real samples

RSC Advances ◽  
2015 ◽  
Vol 5 (64) ◽  
pp. 52095-52100 ◽  
Author(s):  
Alaa S. Amin ◽  
Mohammed A. Kassem ◽  
Talaat Y. Mohammed
Keyword(s):  
Cloud Point ◽  
Absorption Spectra ◽  
Cloud Point Extraction ◽  
Colorimetric Determination ◽  
Real Samples ◽  
P Absorption

Absorption spectra for 0.5 μg mL−1 without CPE and 5.0 ng mL−1 complexed with DMPAHPD using CPE at pH 7.8.

Visible Light Detection of Dopamine Enhanced by Cloud Point Extraction

2019 ◽  
Vol 15 (5) ◽  
pp. 528-534
Author(s):  
Asiye Aslıhan Avan ◽  
Hayati Filik
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Cloud Point ◽  
Cloud Point Extraction ◽  
Extraction Procedure ◽  
Extraction Process ◽  
Forensic Analysis ◽  
Sodium Sulphite ◽  
Pharmaceutical Samples

Background: Monitoring of DA, in the presence of other chemical analogues such as epinephrine, norepinephrine, serotonin, ascorbic acid, uric acid, catechol, phenethylamine, tyramine, and tyrosine, is crucial in the diagnosis and mechanistic understanding of human neuropathology. Therefore, the determination of DA at trace levels has become a very important analytical task, as part of health safety and forensic analysis. Introduction: A cloud point extraction (CPE) process was developed for the isolation and detection of dopamine in food, urine, and pharmaceutical samples. Methods: In this procedure, dopamine was derivatized with o-phthalaldehyde (OPA) and sodium sulphite in aqueous solution. The isoindole derivative was synthesized by the reaction of OPA and sodium sulphite with the amino group of dopamine and the resulted isoindole derivatives were extracted by cloud point extraction. After extraction process, the concentration of enriched analyte was measured by UV-VIS spectrophotometry. The parameters affecting the CPE such as concentration of surfactant and electrolyte, equilibration temperature and time and pH of sample solution were investigated. Results: After optimization of the CPE conditions, the linear range of 8-80 µM (without extraction 100- 1000 µM) was established for dopamine with detection limit at 2.6 µM. Conclusion: The developed extraction procedure was applied to the quantification of dopamine in chocolate, urine, and pharmaceutical samples. The study ensures a promising strategy for the detection of dopamine in the presence of biological constituents, e.g. ascorbic acid, uric acid, and serotonin.

scholarly journals Facile Cloud Point Extraction for the Separation and Determination of Phenolic Acids from Dandelion

ACS Omega ◽  
2021 ◽  
Author(s):  
Yongsheng Ji ◽  
Le Wu ◽  
Ruihong Lv ◽  
Haohao Wang ◽  
Shuhui Song ◽  
...  
Keyword(s):  
Cloud Point ◽  
Phenolic Acids ◽  
Cloud Point Extraction

Determination of the Isoflavone Genistein in Soybeans by High-Performance Liquid Chromatography Following Cloud Point Extraction

2012 ◽  
Vol 95 (3) ◽  
pp. 845-849 ◽  
Author(s):  
Mohammad Mirzaei ◽  
Atieh Karimi Naeini ◽  
Mansoureh Behzadi
Keyword(s):  
Cloud Point ◽  
High Performance ◽  
Cloud Point Extraction ◽  
Uv Detector ◽  
Experimental Conditions ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Replicate Measurements

Abstract A simple and sensitive method has been developed for preconcentration and determination of genistein in soybeans. This method is based on cloud point extraction (CPE) of genistein from soybeans using ethylene glycol monoalkyl ether (Genapol X-080) as a nonionic surfactant. The concentration of extracted genistein was determined by HPLC with a UV detector. Optimum experimental conditions were established. With 5% Genapol X-080 (v/v), a liquid/solid ratio of 25:1 mL/g, and ultrasonic-assisted extraction at 40°C for 45 min, the extraction percentage of genistein reached its highest value. The preconcentration factor for genistein was about 16.5. The RSD for seven replicate measurements and the LOD were ±4.45% and 15.0 ng/mL, respectively. CPE is simple, inexpensive, and suitable for extraction of genistein from soybean. It uses environmentally friendly surfactants and offers a convenient alternative to more conventional extraction systems.

Sign in / Sign up

Export Citation Format

Share Document