scholarly journals Intoxication cases associated with the novel designer drug 3′,4′‐methylenedioxy‐α‐pyrrolidinohexanophenone and studies on its human metabolism using high‐resolution mass spectrometry

2020 ◽  
Vol 12 (9) ◽  
pp. 1320-1335
Author(s):  
Marcel Grapp ◽  
Christoph Kaufmann ◽  
Hannes M. Schwelm ◽  
Merja A. Neukamm ◽  
Sabine Blaschke ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
High Resolution Mass Spectrometry ◽  
Designer Drug ◽  
The Novel ◽  
Human Metabolism ◽  
High Resolution Mass ◽  
Resolution Mass

scholarly journals SYNTHESIS OF 2,2’ – DI-1,3-DIAZOPERENES

2019 ◽  
Author(s):  
М.Х. Магомадова ◽  
А.В. Аксенов ◽  
М.Х. Магомадова ◽  
Р.А. Турлуев ◽  
Э.У. Идрисова
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
High Resolution Mass Spectrometry ◽  
The Novel ◽  
Two Dimensional ◽  
High Resolution Mass ◽  
Oxidative Aromatization ◽  
Novel Method ◽  
Peri Annelation ◽  
Resolution Mass

Разработан новый метод получения 1,3диазапиренов посредством пери-аннелирования пери мидинов 1,3дикарбонильными соединениями. Показано, что такой метод обладает рядом пре имуществ по сравнению с описанным ранее методом Пожарского-Боровлева, использующим халконы в качестве электрофильных компонентов и требующим окислительной ароматизации частично гидрированных промежуточных продуктов. Кроме того, показано, что новый метод от лично применим для эффективного двукратного пери-аннелирования бипeримидинов 1,3ди карбонильными соединениями с целью получения 2,2 би-1,3диазапиренов. Строение 2,2 ди-1,3диазапиренов подтверждено с помощью масс-спектрометрии высокого разрешения, 1Н и 13С ЯМР спектроскопии. В том числе, для этого использовались и двумерные методы HSQCE1H-13C и HMBC1H-13C. Элементный состав соединений определяли при помощи масс-спектрометрии вы сокого разрешения. A novel method of producing 1,3diazapirenes by peri-annelating perimidines with 1,3dicarbonyl compounds has been developed. This method is shown to have a number of advantages over the Pozharsky-Borovlev method described above, which uses halcones as electrophilic components and requires oxidative aromatization of partially hydrogenated intermediates. Furthermore, the novel method has been shown to be excellent for the effective double peri-annelation of the 1, 3dicarbonyl compounds to produce 2, 2 -bi-1,3diazapirenes. The structure of 2,2 -di-1,3diazapirenes was confirmed by high resolution mass spectrometry, 1Н and 13С NMR spectroscopy. This included two-dimensional methods of HSQCE1H-13C and HMBC1H-13C. The elemental composition of the compounds was determined by high resolution mass spectrometry.

Exploring the Active Compounds of Traditional Mongolian Medicine Agsirga in Intervention of Novel Coronavirus (2019-nCoV) Based on HPLC-Q-Exactive-MS/MS and Molecular Docking Method

2020 ◽  
Author(s):  
Jie Cheng ◽  
Yuchen Tang ◽  
Baoquan Bao ◽  
Ping Zhang
Keyword(s):  
Mass Spectrometry ◽  
Molecular Docking ◽  
High Resolution ◽  
Mass Spectra ◽  
High Resolution Mass Spectrometry ◽  
Structural Formula ◽  
Active Compounds ◽  
High Resolution Mass ◽  
Q Exactive ◽  
Resolution Mass

<p><a></a><a></a><a></a><a><b>Objective</b></a>: To screen all compounds of Agsirga based on the HPLC-Q-Exactive high-resolution mass spectrometry and find potential inhibitors that can respond to 2019-nCoV from active compounds of Agsirga by molecular docking technology.</p> <p><b>Methods</b>: HPLC-Q-Exactive high-resolution mass spectrometry was adopted to identify the complex components of Mongolian medicine Agsirga, and separated by the high-resolution mass spectrometry Q-Exactive detector. Then the Orbitrap detector was used in tandem high-resolution mass spectrometry, and the related molecular and structural formula were found by using the chemsipider database and related literature, combined with precise molecular formulas (errors ≤ 5 × 10<sup>−6</sup>) , retention time, primary mass spectra, and secondary mass spectra information, The fragmentation regularities of mass spectra of these compounds were deduced. Taking ACE2 as the receptor and deduced compounds as the ligand, all of them were pretreated by discover studio, autodock and Chem3D. The molecular docking between the active ingredients and the target protein was studied by using AutoDock molecular docking software. The interaction between ligand and receptor is applied to provide a choice for screening anti-2019-nCoV drugs.</p> <p><b>Result</b>: Based on the fragmentation patterns of the reference compounds and consulting literature, a total of 96 major alkaloids and stilbenes were screened and identified in Agsirga by the HPLC-Q-Exactive-MS/MS method. Combining with molecular docking, a conclusion was got that there are potential active substances in Mongolian medicine Agsirga which can block the binding of ACE2 and 2019-nCoV at the molecular level.</p>

Determination of quaternary ammonium compounds in food products by the method of ultra-performance liquid chromatography/high resolution mass-spectrometry

2020 ◽  
Vol 86 (8) ◽  
pp. 23-31
Author(s):  
V. G. Amelin ◽  
D. S. Bolshakov
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Quaternary Ammonium ◽  
High Resolution Mass Spectrometry ◽  
Food Products ◽  
Quaternary Ammonium Compounds ◽  
High Resolution Mass ◽  
Ammonium Compounds ◽  
Resolution Mass

The goal of the study is developing a methodology for determination of the residual amounts of quaternary ammonium compounds (QAC) in food products by UHPLC/high-resolution mass spectrometry after water-acetonitrile extraction of the determined components from the analyzed samples. The identification and determination of QAC was carried out on an «UltiMate 3000» ultra-high-performance liquid chromatograph (Thermo Scientific, USA) equipped with a «maXis 4G» high-resolution quadrupole-time-of-flight mass spectrometric detector and an ion spray «ionBooster» source (Bruker Daltonics, Germany). Samples of milk, cheese (upper cortical layer), dumplings, pork, chicken skin and ground beef were used as working samples. Optimal conditions are specified for chromatographic separation of the mixture of five QAC, two of them being a mixture of homologues with a linear structure (including isomeric forms). The identification of QAC is carried out by the retention time, exact mass of the ions, and coincidence of the mSigma isotopic distribution. The limits for QAC detection are 0.1 – 0.5 ng/ml, the determination limits are 1 ng/ml for aqueous standard solutions. The determinable content of QAC in food products ranges within 1 – 100 ng/g. The results of analysis revealed the residual amount of QAC present in all samples, which confirms data of numerous sources of information about active use of QAC-based disinfectants in the meat and dairy industry. The correctness of the obtained results is verified by introduction of the additives in food products at a level of 10 ng/g for each QAC. The relative standard deviation of the analysis results does not exceed 0.18. The duration of the analysis is 30 – 40 min.

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