Research on spiking rat EPO as internal standard in doping control samples for detection of EPO using SAR‐PAGE analysis with biotinylated primary antibody

2020 ◽  
Vol 12 (8) ◽  
pp. 1054-1064 ◽  
Author(s):  
Xinmiao Zhou ◽  
Sen He ◽  
Lisi Zhang ◽  
Li Shen ◽  
Chunji He
Keyword(s):  
Internal Standard ◽  
Doping Control ◽  
Primary Antibody ◽  
In Doping ◽  
Control Samples

scholarly journals Targeting the administration of ecdysterone in doping control samples

2019 ◽  
Vol 38 (1) ◽  
pp. 172-184 ◽  
Author(s):  
Maria Kristina Parr ◽  
Gabriella Ambrosio ◽  
Bernhard Wuest ◽  
Monica Mazzarino ◽  
Xavier de la Torre ◽  
...  
Keyword(s):  
Doping Control ◽  
In Doping ◽  
Control Samples

scholarly journals Urine Caffeine Concentration in Doping Control Samples from 2004 to 2015

Nutrients ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 286 ◽  
Author(s):  
Millán Aguilar-Navarro ◽  
Gloria Muñoz ◽  
Juan Salinero ◽  
Jesús Muñoz-Guerra ◽  
María Fernández-Álvarez ◽  
...  
Keyword(s):  
Monitoring Program ◽  
Doping Control ◽  
Urine Samples ◽  
Ergogenic Effect ◽  
Caffeine Concentration ◽  
In Doping ◽  
The World ◽  
World Anti Doping Agency ◽  
Individual Sports ◽  
Control Samples

The ergogenic effect of caffeine is well-established, but the extent of its consumption in sport is unknown at the present. The use of caffeine was considered “prohibited” until 2004, but this stimulant was moved from the List of Prohibited Substances to the Monitoring Program of the World Anti-Doping Agency to control its use by monitoring urinary caffeine concentration after competition. However, there is no updated information about the change in the use of caffeine as the result of its inclusion in the Monitoring Program. The aim of this study was to describe the changes in urine caffeine concentration from 2004 to 2015. A total of 7488 urine samples obtained in official competitions held in Spain and corresponding to athletes competing in Olympic sports (2788 in 2004, 2543 in 2008, and 2157 in 2015) were analyzed for urine caffeine concentration. The percentage of samples with detectable caffeine (i.e., >0.1 μg/mL) increased from ~70.1%, in 2004–2008 to 75.7% in 2015. The median urine caffeine concentration in 2015 (0.85 μg/mL) was higher when compared to the median value obtained in 2004 (0.70 μg/mL; p < 0.05) and in 2008 (0.70 μg/mL; p < 0.05). The urine caffeine concentration significantly increased from 2004 to 2015 in aquatics, athletics, boxing, judo, football, weightlifting, and rowing (p < 0.05). However, the sports with the highest urine caffeine concentration in 2015 were cycling, athletics, and rowing. In summary, the concentration of caffeine in the urine samples obtained after competition in Olympic sports in Spain increased from 2004 to 2015, particularly in some disciplines. These data indicate that the use of caffeine has slightly increased since its removal from the list of banned substances, but urine caffeine concentrations suggest that the use of caffeine is moderate in most sport specialties. Athletes of individual sports or athletes of sports with an aerobic-like nature are more prone to using caffeine in competition.

A Review of Analytical Methods for the Determination of Tibolone: Pharmacokinetics and Pharmaceutical Formulations Analysis and Application in Doping Control

2020 ◽  
Vol 17 (1) ◽  
pp. 31-39
Author(s):  
Marilene Lopes Ângelo ◽  
Fernanda de Lima Moreira ◽  
Ana Laura Araújo Santos ◽  
Hérida Regina Nunes Salgado ◽  
Magali Benjamim de Araújo
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Analytical Methods ◽  
Estrogenic Activity ◽  
Biological Fluids ◽  
Pharmaceutical Formulations ◽  
Doping Control ◽  
Specific Effects ◽  
In Doping

Background:: Tibolone is a synthetic steroid commercialized by Organon under the brand name Livial (Org OD14), which is used in hormone therapy for menopause management and treatment of postmenopausal osteoporosis. Tibolone is defined as a selective tissue estrogenic activity regulator (STEAR) demonstrating tissue-specific effects on several organs such as brain, breast, urogenital tract, endometrium, bone and cardiovascular system. Aims:: This work aims to (1) present an overview of important published literature on existing methods for the analysis of tibolone and/or its metabolites in pharmaceutical formulations and biological fluids and (2) to conduct a critical comparison of the analytical methods used in doping control, pharmacokinetics and pharmaceutical formulations analysis of tibolone and its metabolites. Results and conclusions: : The major analytical method described for the analysis of tibolone in pharmaceutical formulations is High Pressure Liquid Chromatography (HPLC) coupled with ultraviolet (UV) detection, while Liquid Chromatography (LC) or Gas Chromatography (GC) used in combination with Mass Spectrometry (MS) or tandem mass spectrometry (MS/MS) is employed for the analysis of tibolone and/or its metabolites in biological fluids.

Qualitative screening in doping control by MALDI-TOF/TOF mass spectrometry: A proof-of-evidence

2012 ◽  
Vol 71 ◽  
pp. 193-197 ◽  
Author(s):  
E. Galano ◽  
M. Fidani ◽  
F. Baia ◽  
L. Palomba ◽  
G. Marino ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Doping Control ◽  
Maldi Tof ◽  
In Doping

Application of the new ion trap detector in doping control

1988 ◽  
Vol 6 (6-8) ◽  
pp. 987-993
Author(s):  
E.G. de Jong ◽  
R.A.A. Maes ◽  
J.M. van Rossum
Keyword(s):  
Ion Trap ◽  
Doping Control ◽  
Trap Detector ◽  
In Doping

Evaluation of AMGEN clone 9G8A anti-Epo antibody for application in doping control

2016 ◽  
Vol 8 (11-12) ◽  
pp. 1131-1137 ◽  
Author(s):  
Christian Reichel ◽  
Wolfgang Benetka ◽  
Barbara Lorenc ◽  
Mario Thevis
Keyword(s):  
Doping Control ◽  
In Doping
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