Comparative study on intestinal metabolism and absorptionin vivoof ginsenosides in sulphur-fumigated and non-fumigated ginseng by ultra performance liquid chromatography quadruple time-of-flight mass spectrometry based chemical profiling approach

2014 ◽  
Vol 7 (4) ◽  
pp. 320-330 ◽  
Author(s):  
He Zhu ◽  
Hong Shen ◽  
Jun Xu ◽  
Jin-Di Xu ◽  
Ling-Ying Zhu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Comparative Study ◽  
Time Of Flight ◽  
Ultra Performance Liquid Chromatography ◽  
Intestinal Metabolism ◽  
Chemical Profiling ◽  
Flight Mass Spectrometry

scholarly journals Rapid Evaluation of Chemical Consistency of Artificially Induced and Natural Resina Draconis Using Ultra-Performance Liquid Chromatography Quadrupole-Time-of-Flight Mass Spectrometry-Based Chemical Profiling

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 1850 ◽  
Author(s):  
Qianping Chen ◽  
Lili He ◽  
Changming Mo ◽  
Zhifeng Zhang ◽  
Hairong Long ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Chemical Constituents ◽  
Time Of Flight ◽  
Ultra Performance Liquid Chromatography ◽  
Market Demand ◽  
Chemical Profiling ◽  
Flight Mass Spectrometry ◽  
Chromatographic Peaks ◽  
Resina Draconis

Resina Draconis is a highly valued traditional medicine widely used in Arabia since ancient times, and it has been commonly used as an antidiarrheic, antimicrobial, antiulcer, blood circulation promoter as well as an anti-inflammatory agent. The tree source from which this medicine orignates grows extremely slowly, producing a very low yield of Resina Draconis. To meet the increasing market demand, artificial methods for stimulating Resina Draconis formation have been developed and applied. However, the chemical differences between artificially induced Resina Draconis (AIRD) and natural Resina Draconis (NRD) have been rarely studied. The aim of this research was to explore and identify the chemical constituents of AIRD and NRD using ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UHPLC-QTOF-MS/MS) based chemical profiling. A total of 56 chromatographic peaks were detected in AIRD, of these, 44 peaks have had their structures tentatively characterized based on high-resolution mass spectra (HRMS) data, fragmentation ions information, reference standards data and literature review. In total, 40 peaks were found both in AIRD and NRD. The potential chemical transformation mechanisms active in Resina Draconis during formation were explored. To the best of our knowledge, this is the first evaluation of the chemical profiles of both AIRD and NRD. Furthermore, these findings are expected to provide a rational basis for the quality assessment of AIRD and the use of AIRD as a substitute for NRD.

Chemical profiling of Di-Wu-Yang-Gan Granules by ultra performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry with MSE technology

2018 ◽  
Vol 73 (3-4) ◽  
pp. 107-116
Author(s):  
Dong Tian ◽  
Chao Zhou ◽  
Hong-Mei Jia ◽  
Meng Yu ◽  
Xing Chang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Chemical Constituents ◽  
Time Of Flight ◽  
Herbal Medicines ◽  
Ultra Performance Liquid Chromatography ◽  
Chinese Herbs ◽  
Chemical Information ◽  
Chemical Profiling ◽  
Flight Mass Spectrometry

AbstractDi-Wu-Yang-Gan Granules is a Traditional Chinese Medicine prescription used for the treatment of HBeAg-negative chronic hepatitis B patients in China. It consists of five commonly used Chinese herbs. However, the chemical constituents of the whole prescription had not been clarified yet. Hence, in this study, the chemical profiling of Di-Wu-Yang-Gan Granules was explored by ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry, which can provide accurate molecular weight within 5-ppm error and sufficient MS/MS fragment ions without the need for precursor ion selection. As a result, 116 compounds were identified, including lignans, triterpenesaponins, flavonoids, coumarins, iridoids, nortriterpenoids, phenolic acids, and sesquiterpenes. All compounds were further assigned to the individual herbs. In conclusion, this established method was reliable and effective for the separation and identification of the constituents in Di-Wu-Yang-Gan Granules. The findings are beneficial for quality control of the prescription during production and provide helpful chemical information for exploring its efficacy and the mechanism of action. The fragmentation regularity summarized in this study also provided important information for the rapid identification of the chemical composition in herbal medicines or their prescription.

Comparative Analysis of the Chemical Consistency Between the Traditional and Mixed Decoction of Maimendong Decoction by Ultra-Performance Liquid Chromatography Coupled to Quadrupole with Time-of-Flight Mass Spectrometry (UPLC–QTOF-MS)-Based Chemical Profiling Approach

2020 ◽  
Vol 58 (6) ◽  
pp. 549-561 ◽  
Author(s):  
Lan Yang ◽  
Zhenhua Zhu ◽  
Zhonghua Qi ◽  
Xinsheng Fan ◽  
Dawei Qian ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Time Of Flight ◽  
Ultra Performance Liquid Chromatography ◽  
Negative Ion ◽  
Chemical Profiling ◽  
Flight Mass Spectrometry ◽  
Traditional Preparation ◽  
Ginsenoside Rg5 ◽  
Negative Ion Mode

Abstract Take Maimendong Decoction (MMDD), one of the Chinese classic herbal formulas, as an object to evaluate the chemical consistency between traditional decoction and mixed decoction. The ultra-performance liquid chromatography coupled to quadrupole with time-of-flight mass spectrometry-based chemical profiling approach has been utilized. A total of 48 major peaks are detected from these two decoctions under the present chromatographic and mass spectrometry conditions. The results of negative ion mode show nine significant inconsistencies. Liquiritin, ginsenoside Ro and ginsenoside Rg5/Rk1 are detected with higher intensity in traditional preparation sample than the mixed decoction, while licoisoflavone A is higher in mixed decoction samples than the traditional one. The mechanisms involved in the chemical changes were assumed to be anti-inflammation, anti-oxidative stress and so on, suggesting these two different preparation approaches of MMDD may lead to a possibility of discrepant clinical outcomes.

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