Microglia of medicinal leech (Hirudo medicinalis) express a specific activation marker homologous to vertebrate ionized calcium-binding adapter molecule 1 (Iba1/alias aif-1)

2014 ◽  
Vol 74 (10) ◽  
pp. 987-1001 ◽  
Author(s):  
Francesco Drago ◽  
Pierre-Eric Sautière ◽  
Françoise Le Marrec-Croq ◽  
Alice Accorsi ◽  
Christelle Van Camp ◽  
...  
Keyword(s):  
Calcium Binding ◽  
Medicinal Leech ◽  
Hirudo Medicinalis ◽  
Ionized Calcium ◽  
Activation Marker ◽  
Adapter Molecule

scholarly journals A Stereological Analysis of NPY, POMC, Orexin, GFAP Astrocyte, and Iba1 Microglia Cell Number and Volume in Diet-Induced Obese Male Mice

Endocrinology ◽  
2015 ◽  
Vol 156 (5) ◽  
pp. 1701-1713 ◽  
Author(s):  
Moyra B. Lemus ◽  
Jacqueline A. Bayliss ◽  
Sarah H. Lockie ◽  
Vanessa V. Santos ◽  
Alex Reichenbach ◽  
...  
Keyword(s):  
Glial Fibrillary Acidic Protein ◽  
Cell Volume ◽  
Lateral Hypothalamus ◽  
Calcium Binding ◽  
Cell Number ◽  
Ionized Calcium ◽  
Volume Distribution ◽  
Stereological Analysis ◽  
Adapter Molecule ◽  
Neuronal Number

The hypothalamic arcuate nucleus (ARC) contains 2 key neural populations, neuropeptide Y (NPY) and proopiomelanocortin (POMC), and, together with orexin neurons in the lateral hypothalamus, plays an integral role in energy homeostasis. However, no studies have examined total neuronal number and volume after high-fat diet (HFD) exposure using sophisticated stereology. We used design-based stereology to estimate NPY and POMC neuronal number and volume, as well as glial fibrillary acidic protein (astrocyte marker) and ionized calcium-binding adapter molecule 1 (microglia marker) cell number in the ARC; as well as orexin neurons in the lateral hypothalamus. Stereological analysis indicated approximately 8000 NPY and approximately 9000 POMC neurons in the ARC, and approximately 7500 orexin neurons in the lateral hypothalamus. HFD exposure did not affect total neuronal number in any population. However, HFD significantly increased average NPY cell volume and affected NPY and POMC cell volume distribution. HFD reduced orexin cell volume but had a bimodal effect on volume distribution with increased cells at relatively small volumes and decreased cells with relatively large volumes. ARC glial fibrillary acidic protein cells increased after 2 months on a HFD, although no significant difference after 6 months on chow diet or HFD was observed. No differences in ARC ionized calcium-binding adapter molecule 1 cell number were observed in any group. Thus, HFD affects ARC NPY or POMC neuronal cell volume number not cell number. Our results demonstrate the importance of stereology to perform robust unbiased analysis of cell number and volume. These data should be an empirical baseline reference to which future studies are compared.

scholarly journals Spermatid-Specific Expression of Iba1, an Ionized Calcium Binding Adapter Molecule-1, in Rat Testis1

2001 ◽  
Vol 64 (4) ◽  
pp. 1138-1146 ◽  
Author(s):  
Hiroshi Iida ◽  
Masamichi Doiguchi ◽  
Haruhiro Yamashita ◽  
Shinsaku Sugimachi ◽  
Junko Ichinose ◽  
...  
Keyword(s):  
Calcium Binding ◽  
Ionized Calcium ◽  
Specific Expression ◽  
Adapter Molecule

Comparison of Ionized Calcium-binding Adapter Molecule 1 Immunoreactivity of the Hippocampal Dentate Gyrus and CA1 Region in Adult and Aged Dogs

2008 ◽  
Vol 33 (7) ◽  
pp. 1309-1315 ◽  
Author(s):  
In Koo Hwang ◽  
Choong Hyun Lee ◽  
Hua Li ◽  
Ki-Yeon Yoo ◽  
Jung Hoon Choi ◽  
...  
Keyword(s):  
Dentate Gyrus ◽  
Calcium Binding ◽  
Ionized Calcium ◽  
Hippocampal Dentate Gyrus ◽  
Ca1 Region ◽  
Adapter Molecule

Age-related changes in ionized calcium-binding adapter molecule 1 in the gerbil hippocampal CA1 region

2007 ◽  
Vol 58 ◽  
pp. S74
Author(s):  
Jung Hoon Choi ◽  
Choong Hyun Lee ◽  
Dae Won Chung ◽  
In Ku Hwang ◽  
Moo Ho Won ◽  
...  
Keyword(s):  
Calcium Binding ◽  
Ionized Calcium ◽  
Ca1 Region ◽  
Hippocampal Ca1 Region ◽  
Age Related ◽  
Hippocampal Ca1 ◽  
Adapter Molecule ◽  
Age Related Changes

scholarly journals Glial Fibrillary Acidic Protein and Ionized Calcium-Binding Adapter Molecule 1 Immunostaining Score for the Central Nervous System of Horses With Non-suppurative Encephalitis and Encephalopathies

2021 ◽  
Vol 8 ◽  
Author(s):  
Gisele Silva Boos ◽  
Klaus Failing ◽  
Edson Moleta Colodel ◽  
David Driemeier ◽  
Márcio Botelho de Castro ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Glial Fibrillary Acidic Protein ◽  
Calcium Binding ◽  
Acidic Protein ◽  
Ionized Calcium ◽  
Morphological Pattern ◽  
The Central Nervous System ◽  
Adapter Molecule ◽  
Cell Alterations

Like humans, horses are susceptible to neurotropic and neuroinvasive pathogens that are not always readily identified in histological sections. Instead, alterations in astrocytes and microglia cells can be used as pathological hallmarks of injured nervous tissue in a variety of infectious and degenerative diseases. On the other hand, equine glial cell alterations are poorly characterized in diseases. Therefore, in this study, we provide a statistically proved score system to classify astrogliosis and microgliosis in the central nervous system (CNS) of horses, based on morphological and quantitative analyses of 35 equine cases of encephalitis and/or encephalopathies and four non-altered CNS as controls. For this system, we used glial fibrillary acidic protein (GFAP) and ionized calcium-binding adapter molecule 1 (Iba1) immunohistochemistry, allied to statistical analysis to confirm that the scores were correctly designated. The scores of alterations ranged from 0 (non-altered) to 3 (severely altered) and provided a helpful method for describing astrocytic and microglial alterations in horses suffering from inflammatory and degenerative lesions. This system could be a template for comparative studies in other animal species and could aid algorithms designed for artificial intelligence methods lacking a defined morphological pattern.

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