Mutagenesis studies in transgenicXenopus intermediate pituitary cells reveal structural elements necessary for correct prion protein biosynthesis

2007 ◽  
Vol 67 (6) ◽  
pp. 715-727
Author(s):  
Jos W.G. van Rosmalen ◽  
Gerard J.M. Martens
Keyword(s):  
Prion Protein ◽  
Protein Biosynthesis ◽  
Pituitary Cells ◽  
Structural Elements

scholarly journals Backbone NMR assignments of the C-terminal domain of the human prion protein and its disease‐associated T183A variant

2021 ◽  
Vol 15 (1) ◽  
pp. 193-196
Author(s):  
Máximo Sanz-Hernández ◽  
Alfonso De Simone
Keyword(s):  
Prion Protein ◽  
Nmr Assignments ◽  
Chemical Shifts ◽  
Prion Diseases ◽  
Random Coil ◽  
Transmissible Spongiform Encephalopathies ◽  
Globular Domain ◽  
Structural Elements ◽  
Spongiform Encephalopathies ◽  
Human Prion Protein

AbstractTransmissible spongiform encephalopathies (TSEs) are fatal neurodegenerative disorders associated with the misfolding and aggregation of the human prion protein (huPrP). Despite efforts into investigating the process of huPrP aggregation, the mechanisms triggering its misfolding remain elusive. A number of TSE-associated mutations of huPrP have been identified, but their role at the onset and progression of prion diseases is unclear. Here we report the NMR assignments of the C-terminal globular domain of the wild type huPrP and the pathological mutant T183A. The differences in chemical shifts between the two variants reveal conformational alterations in some structural elements of the mutant, whereas the analyses of secondary shifts and random coil index provide indications on the putative mechanisms of misfolding of T183A huPrP.

Transgene expression of prion protein induces crinophagy in intermediate pituitary cells

2007 ◽  
Vol 67 (1) ◽  
pp. 81-96 ◽  
Author(s):  
Jos W.G. van Rosmalen ◽  
Gerard J.M. Martens
Keyword(s):  
Prion Protein ◽  
Transgene Expression ◽  
Pituitary Cells

scholarly journals Interactions of Calix[n]arenes with Nucleic Acids

2012 ◽  
Vol 7 (3) ◽  
pp. 1934578X1200700
Author(s):  
Max Sena Peters ◽  
Miao Li ◽  
Thomas Schrader
Keyword(s):  
Nucleic Acids ◽  
Early Stage ◽  
Protein Biosynthesis ◽  
Biological Effects ◽  
Dna Interaction ◽  
Host Cells ◽  
Supramolecular Interactions ◽  
Structural Elements ◽  
Molecular Scaffolds ◽  
Dna Strands

DNA interaction with artificial binders is of great interest, especially in light of the broad range of possible biomedical applications. The growing understanding of replication, transcription and translation opened the path for new approaches to target pathological effects at a very early stage. Meanwhile, the competitive binding to nucleic acids by designed molecules, which, for example, block certain sequences for natural binders, such as transcription factors, has become a promising concept in the context of gene therapy. On the other extreme, the transport of nucleic acids over the cell membrane into the nucleus by transfection agents opens the possibility to reprogram protein biosynthesis within host cells. In the past decades several substance classes have been developed for a noncovalent specific DNA binding with predictable biological effects, such as peptide nucleic acids or polyamide ligands. Calixarenes have not received so much attention, although they consist of a compact aromatic core tuneable in size, and allow the introduction of cationic functionalities at their upper and lower rims. Formerly being utilized as receptor moieties due to the possibility of complexating guests in their cavities, calixarenes are now also used as molecular scaffolds for multivalent ligands and are, therefore, suitable tools for cooperative DNA complexation. This review surveys specific supramolecular interactions between calixarene derivatives and nucleic acids, with an emphasis on structural elements in the calixarenes and the biological consequences of their complex formation with DNA strands.

scholarly journals Prion protein biosynthesis and its emerging role in neurodegeneration

2009 ◽  
Vol 34 (6) ◽  
pp. 287-295 ◽  
Author(s):  
Oishee Chakrabarti ◽  
Aarthi Ashok ◽  
Ramanujan S. Hegde
Keyword(s):  
Prion Protein ◽  
Protein Biosynthesis

scholarly journals Prion protein biosynthesis in scrapie-infected and uninfected neuroblastoma cells.

1989 ◽  
Vol 63 (1) ◽  
pp. 175-181 ◽  
Author(s):  
B Caughey ◽  
R E Race ◽  
D Ernst ◽  
M J Buchmeier ◽  
B Chesebro
Keyword(s):  
Prion Protein ◽  
Protein Biosynthesis ◽  
Neuroblastoma Cells

scholarly journals Most of the structural elements of the globular domain of murine prion protein form fibrils with predominant β-sheet structure

FEBS Letters ◽  
2002 ◽  
Vol 529 (2-3) ◽  
pp. 256-260 ◽  
Author(s):  
Nadège Jamin ◽  
Yves-Marie Coı̈c ◽  
Céline Landon ◽  
Ludmila Ovtracht ◽  
Françoise Baleux ◽  
...  
Keyword(s):  
Prion Protein ◽  
Globular Domain ◽  
Structural Elements ◽  
Sheet Structure ◽  
Β Sheet

Transgene expression of prion protein induces crinophagy in intermediate pituitary cells

2006 ◽  
Vol 67 (1) ◽  
pp. 81-96 ◽  
Author(s):  
Jos W.G. van Rosmalen ◽  
Gerard J.M. Martens
Keyword(s):  
Prion Protein ◽  
Transgene Expression ◽  
Pituitary Cells
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