scholarly journals Constant and intermittent high glucose enhances endothelial cell apoptosis through mitochondrial superoxide overproduction

2006 ◽  
Vol 22 (3) ◽  
pp. 198-203 ◽  
Author(s):  
Ludovica Piconi ◽  
Lisa Quagliaro ◽  
Roberta Assaloni ◽  
Roberto Da Ros ◽  
Amabile Maier ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
High Glucose ◽  
Cell Apoptosis ◽  
Endothelial Cell Apoptosis ◽  
Mitochondrial Superoxide ◽  
Intermittent High Glucose

scholarly journals Taurine prevents high-glucose-induced human vascular endothelial cell apoptosis

1999 ◽  
Vol 277 (6) ◽  
pp. C1229-C1238 ◽  
Author(s):  
Qiong di Wu ◽  
Jiang Huai Wang ◽  
Fiona Fennessy ◽  
H. Paul Redmond ◽  
David Bouchier-Hayes
Keyword(s):  
Endothelial Cell ◽  
High Glucose ◽  
Cell Apoptosis ◽  
Umbilical Vein ◽  
Vascular Endothelial Cell ◽  
Microvascular Disease ◽  
Diabetic Microangiopathy ◽  
Critical Event ◽  
Endothelial Cell Apoptosis ◽  
Human Vascular Endothelial Cell

Elevated blood glucose in uncontrolled diabetes is causally correlated with diabetic microangiopathy. Hyperglycemia-triggered accelerated endothelial cell apoptosis is a critical event in the process of diabetes-associated microvascular disease. The conditionally semiessential amino acid taurine has been previously shown to protect against human endothelial cell apoptosis. Therefore, this study was designed to investigate the role of taurine in the prevention of high-glucose-mediated cell apoptosis in human umbilical vein endothelial cells (HUVEC) and the mechanisms involved. Exposure of HUVEC to 30 mM glucose for 48 h (short-term) and 14 days (long-term) resulted in a significant increase in apoptosis, compared with normal glucose (5.5 mM; P < 0.05). High-glucose-induced DNA fragmentation preferentially occurred in the S phase cells. Mannitol (as osmotic control) at 30 mM failed to induce HUVEC apoptosis. Taurine prevented high-glucose-induced HUVEC apoptosis, which correlates with taurine attenuation of high-glucose-mediated increased intracellular reactive oxygen species (ROS) formation and elevated intracellular Ca2+ concentration ([Ca2+]i) level. Antioxidants, DMSO, N-acetyl cysteine, and glutathione, only partly attenuated high-glucose-induced HUVEC apoptosis. Glucose at 30 mM did not cause HUVEC necrosis. However, both glucose and mannitol at 60 mM caused HUVEC necrosis as represented by increased lactate dehydrogenase release and cell lysis. Taurine failed to prevent hyperosmolarity-induced cell necrosis. These results demonstrate that taurine attenuates hyperglycemia-induced HUVEC apoptosis through ROS inhibition and [Ca2+]istabilization and suggest that taurine may exert a beneficial effect in preventing diabetes-associated microangiopathy.

Critical effect of VEGF in the process of endothelial cell apoptosis induced by high glucose

APOPTOSIS ◽  
2008 ◽  
Vol 13 (11) ◽  
pp. 1331-1343 ◽  
Author(s):  
Zhonghan Yang ◽  
Xuehua Mo ◽  
Qing Gong ◽  
Qiuhui Pan ◽  
Xia Yang ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
High Glucose ◽  
Cell Apoptosis ◽  
Endothelial Cell Apoptosis ◽  
Critical Effect

Effect of erythropoietin on endothelial cell apoptosis induced by high glucose

2004 ◽  
Vol 66 ◽  
pp. S103-S107 ◽  
Author(s):  
Naotaka Sekiguchi ◽  
Toyoshi Inoguchi ◽  
Kunihisa Kobayashi ◽  
Hajime Nawata
Keyword(s):  
Endothelial Cell ◽  
High Glucose ◽  
Cell Apoptosis ◽  
Endothelial Cell Apoptosis
Sign in / Sign up

Export Citation Format

Share Document