Biphasic effects of chronic ethanol exposure on insulin-stimulated glucose uptake in primary cultured rat skeletal muscle cells: role of the Akt pathway and GLUT4

2010 ◽  
Vol 27 (1) ◽  
pp. 47-53 ◽  
Author(s):  
Wei Qu ◽  
Lina Zhao ◽  
Xiaolin Peng ◽  
Xuefeng Yang ◽  
Chenjiang Ying ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Glucose Uptake ◽  
Muscle Cells ◽  
Ethanol Exposure ◽  
Chronic Ethanol ◽  
Skeletal Muscle Cells ◽  
Akt Pathway ◽  
Rat Skeletal Muscle ◽  
Chronic Ethanol Exposure

scholarly journals In vitro anti-diabetic assessment of guavanoic acid functionalized gold nanoparticles in regulating glucose transport using L6 rat skeletal muscle cells

2020 ◽  
Vol 11 (7) ◽  
pp. 814-822
Author(s):  
K. Govindaraju ◽  
K. S. Uma Suganya
Keyword(s):  
Skeletal Muscle ◽  
Gold Nanoparticles ◽  
Glucose Uptake ◽  
Glucose Transport ◽  
Muscle Cells ◽  
Pi3k Inhibitor ◽  
Skeletal Muscle Cells ◽  
Rat Skeletal Muscle ◽  
Functionalized Gold Nanoparticles

Glucose uptake patterns of guavanoic acid and guavanoic acid functionalized gold nanoparticles in the presence of genistein (IRTK inhibitor) and wortmannin (PI3K inhibitor).

scholarly journals The exocyst complex regulates insulin-stimulated glucose uptake of skeletal muscle cells

2019 ◽  
Vol 317 (6) ◽  
pp. E957-E972
Author(s):  
Brent A. Fujimoto ◽  
Madison Young ◽  
Lamar Carter ◽  
Alina P. S. Pang ◽  
Michael J. Corley ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Plasma Membrane ◽  
Cell Surface ◽  
Glucose Uptake ◽  
Glucose Transporter ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Cell Surface Receptor ◽  
Exocyst Complex

Skeletal muscle handles ~80–90% of the insulin-induced glucose uptake. In skeletal muscle, insulin binding to its cell surface receptor triggers redistribution of intracellular glucose transporter GLUT4 protein to the cell surface, enabling facilitated glucose uptake. In adipocytes, the eight-protein exocyst complex is an indispensable constituent in insulin-induced glucose uptake, as it is responsible for the targeted trafficking and plasma membrane-delivery of GLUT4. However, the role of the exocyst in skeletal muscle glucose uptake has never been investigated. Here we demonstrate that the exocyst is a necessary factor in insulin-induced glucose uptake in skeletal muscle cells as well. The exocyst complex colocalizes with GLUT4 storage vesicles in L6-GLUT4myc myoblasts at a basal state and associates with these vesicles during their translocation to the plasma membrane after insulin signaling. Moreover, we show that the exocyst inhibitor endosidin-2 and a heterozygous knockout of Exoc5 in skeletal myoblast cells both lead to impaired GLUT4 trafficking to the plasma membrane and hinder glucose uptake in response to an insulin stimulus. Our research is the first to establish that the exocyst complex regulates insulin-induced GLUT4 exocytosis and glucose metabolism in muscle cells. A deeper knowledge of the role of the exocyst complex in skeletal muscle tissue may help our understanding of insulin resistance in type 2 diabetes.

scholarly journals Bioactives of Artemisia dracunculus L. Mitigate the Role of Ceramides in Attenuating Insulin Signaling in Rat Skeletal Muscle Cells

Diabetes ◽  
2012 ◽  
Vol 61 (3) ◽  
pp. 597-605 ◽  
Author(s):  
D. N. Obanda ◽  
A. Hernandez ◽  
D. Ribnicky ◽  
Y. Yu ◽  
X. H. Zhang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Insulin Signaling ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Rat Skeletal Muscle ◽  
Artemisia Dracunculus

scholarly journals AN IN VITRO STUDY OF SYZYGIUM CUMINI SEED EXTRACT ON GLUCOSE UPTAKE ACTIVITY IN L-6 CELL LINES

2019 ◽  
Vol 9 (4-A) ◽  
pp. 256-259
Author(s):  
Maneemegalai Sivaprakasam ◽  
Narmatha M
Keyword(s):  
Diabetes Mellitus ◽  
Skeletal Muscle ◽  
Glucose Uptake ◽  
Ethanol Extract ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Syzygium Cumini ◽  
Rat Skeletal Muscle ◽  
Uptake Activity

Diabetes mellitus is the most common endocrine disorder. The plant Syzygium cumini has been used in traditional medicine for the treatment of diabetes. The present study investigated the effect of ethanol extract of S. cumini seeds on uptake of glucose by L-6 rat skeletal muscle cells. S. cumini seeds were extracted with varying solvents and quantitative phytochemical analysis was carried out, ethanol extract of seeds exhibited higher content of tested phytochemicals. The effect of different concentrations (300µg/ml – 1000µg/ml) of ethanol extract of seeds were studied on glucose uptake activity of L-6 rat skeletal muscle cells. It was observed that with the increase in concentration, the glucose uptake activity was also increased. The results of the study supports and demonstrates the antidiabetic potential of ethanol seed extracts of Syzygium cumini utilizing in vitro model. KEY WORDS: Diabetes mellitus, Syzygium cumini, phytochemicals, glucose uptake, L-6 cells

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