Effects of sinomenine on the proliferation, cytokine production, and regulatory T‐cell frequency in peripheral blood mononuclear cells of rheumatoid arthritis patients

2020 ◽  
Author(s):  
Wencheng Xu ◽  
Shuhe Chen ◽  
Xiaoqin Wang ◽  
Hongguang Wu ◽  
Koichiro Tahara ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cell ◽  
Peripheral Blood Mononuclear Cells ◽  
Regulatory T Cell ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Cytokine Production ◽  
Cell Frequency ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals Reduction of Oxidative Stress in Peripheral Blood Mononuclear Cells Attenuates the Inflammatory Response of Fibroblast-like Synoviocytes in Rheumatoid Arthritis

2021 ◽  
Vol 22 (22) ◽  
pp. 12411
Author(s):  
Ha-Reum Lee ◽  
Su-Jin Yoo ◽  
Jinhyun Kim ◽  
Chan Keol Park ◽  
Seong Wook Kang
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cell ◽  
Inflammatory Response ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
T Cell Subsets ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Fibroblast Like Synoviocytes

The production and oxidation mechanism of reactive oxygen species (ROS) are out of balance in rheumatoid arthritis (RA). However, the correlation between ROS and T cell subsets in RA remains unclear. Peripheral blood mononuclear cells (PBMCs) from patients with RA (n = 40) and healthy controls (n = 10) were isolated from whole blood samples. Synovial tissues (n = 3) and synovial fluid (n = 10) were obtained from patients with RA. The repartition of T cell subsets and expression of ROS and cytokines were examined according to RA severity. Fibroblast-like synoviocytes (FLSs) from patients with RA were stimulated with PBMCs and the expression of inflammation-related molecules were measured by RT-PCR and cytokine array. Regulatory T cells from patients with moderate (5.1 > DAS28 ≥ 3.2) RA showed the highest expression of mitochondrial ROS among the groups based on disease severity. Although ROS levels steadily increased with RA severity, there was a slight decline in severe RA (DAS28 ≥ 5.1) compared with moderate RA. The expression of inflammatory cytokines in RA FLSs were significantly inhibited when FLSs were co-cultured with PBMCs treated with ROS inhibitor. These findings provide a novel approach to suppress inflammatory response of FLSs through ROS regulation in PBMCs.

Sign in / Sign up

Export Citation Format

Share Document