scholarly journals Spontaneous mutations conferring antibiotic resistance to antitubercular drugs at a range of concentrations inMycobacterium smegmatis

2018 ◽  
Vol 80 (1) ◽  
pp. 147-154 ◽  
Author(s):  
Iveren Winifred Nyinoh
Keyword(s):  
Antibiotic Resistance ◽  
Spontaneous Mutations ◽  
Antitubercular Drugs

scholarly journals Spontaneous mutations conferring antibiotic resistance to antitubercular drugs at a range of concentrations in Mycobacterium smegmatis

2018 ◽  
Author(s):  
Iveren W Nyinoh ◽  
Johnjoe McFadden
Keyword(s):  
Antibiotic Resistance ◽  
Mutation Rate ◽  
Mycobacterium Smegmatis ◽  
Future Generations ◽  
Mutation Rates ◽  
Fluctuation Analysis ◽  
Spontaneous Mutations ◽  
Likelihood Estimator ◽  
Antitubercular Drugs

AbstractMycobacteria population can undergo mutations in their DNA sequence during replication, which if not repaired, would be transferred to future generations. In this study, in vitro spontaneous mutations in Mycobacterium smegmatis mc2155 (Msm) conferring resistance to isoniazid (INHr), rifampicin (RIFr), kanamycin (KANr) and streptomycin (STRr) were determined at several concentrations in a fluctuation assay. Mutation rate was estimated using the P₀ method, and estimates were then compared with the Lea-Coulson method of the median and Ma-Sandri-Sarkar Maximum Likelihood Estimator (MSS-MLE) method available on the Fluctuation analysis calculator (FALCOR). The mutation rates of RIFr ranged from 9.24 × 10-8 - 2.18 × 10-10, INHr 1.2 × 10-7 - 1.20×10-9, STRr 2.77 × 10-8 - 5.31 × 10-8 and KANr 1.7 × 10-8 mutations per cell division. This study provides mutation rate estimates to key antitubercular drugs at a range of concentrations.

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